In Vitro Hepatotoxicity Testing
Benefits of Using HeptoGlo™-Tox HT for In Vitro Hepatotoxicity Testing
- Hepatocytes produce high concentrations of intracellular ATP (iATP) due to their high metabolic activity. Changes in iATP concentration directly correlate with cellular and mitochondrial integrity and cytotoxicity.
- Both primary and iPS-derived hepatocytes can be used with HepatoGlo™-Tox HT.
- HepatoGlo™-Tox HT incorporates a validated ATP bioluminescence readout to measure hepatotoxicity.
- HepatoGlo™-Tox HT is a calibrated and standardized assay platform that allows results to be directly compared between different drugs, hepatocyte sources and species over time.
- High-throughput capability using 96- or 384-well plate formats allows ADME-Tox drug or compound screening, thereby significantly reducing unexpected results during pre-clinical testing.
- Incorporates specialized HepatoGro™ medium and culture protocols.
- 2D and 3D hepatotoxicity models available.
- Hepatotoxicity contract studies can be performed using different oxygen tensions that simulate different conditions and areas in the liver.
- Available for adherent or non-adherent cell populations.
- HepatoGlo™-Tox HT can be used to investigate cellular drug-drug interactions or multiplexed with CYP450 studies at the sub-cellular level.
- Incorporates the most sensitive ATP bioluminescence readout available.
- Incubation times: Usually 4-24 hours.
- Turnaround time: Usually within 1-3 days.
- Validated assay readout according to FDA Bioanalytical Method Guidelines.
- Designed for multiplexing with other assays using the same sample thereby allowing multiple and liver-specific readouts.
- A 3Rs Assay Platform-Helps Reduction, Refinement, Replacement of animal testing.
Hepatocyte Sources for HepatoGlo™-Tox HT
- Individual or pooled
- ES- and/or iPS-derived
- Hepatocyte cell lines e.g. HepG2
Species Available for Use with HepatoGlo™-Tox HT
- Non-human primate
Assays that can be Multiplexed with HepatoGlo™-Tox HT
- Cytochrome P450 enzyme assays.
- Cellular drug-drug interaction assays.
- Membrane integrity: LDH or PI dye exclusion.
- Apoptosis: Biochemical caspase detection.
- Mitochondrial dysfunction: Mitochondrial ToxGlo™
- Glutathione Assay (GSH): Oxidative stress.
- OxyFLOW™: Oxidative DNA damage.