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In Vitro Hepatotoxicity Testing

 

HepatoGlo-Tox HT: An in vitro hepatotoxicity assay  

 Benefits
  • Hepatocytes produce high concentrations of intracellular ATP (iATP) due to their high metabolic activity. Changes in iATP concentration directly correlate with cellular and mitochondrial integrity and cytotoxicity.
  • Both primary and iPS-derived hepatocytes can be used with HepatoGlo™-Tox HT.
  • HepatoGlo™-Tox HT incorporates a validated ATP bioluminescence readout to measure hepatotoxicity.
  • HepatoGlo™-Tox HT is a calibrated and standardized assay platform that allows results to be directly compared between different drugs, hepatocyte sources and species over time.
  • High-throughput capability using 96- or 384-well plate formats allows ADME-Tox drug or compound screening, thereby significantly reducing unexpected results during pre-clinical testing.
  • Incorporates specialized HepatoGro™ medium and culture protocols.
  • 2D and 3D hepatotoxicity models available.
  • Hepatotoxicity contract studies can be performed using different oxygen tensions that simulate different conditions and areas in the liver.
  • Available for adherent or non-adherent cell populations.
  • HepatoGlo™-Tox HT can be used to investigate cellular drug-drug interactions or multiplexed with CYP450 studies at the sub-cellular level.
  • Incorporates the most sensitive ATP bioluminescence readout available.
  • Incubation times: Usually 4-24 hours.
  • Turnaround time: Usually within 1-3 days.
  • Validated assay readout according to FDA Bioanalytical Method Guidelines.
  • Designed for multiplexing with other assays using the same sample thereby allowing multiple and liver-specific readouts.
  • A 3Rs Assay Platform-Helps Reduction, Refinement, Replacement of animal testing.
 Hepatocyte Sources for HepatoGlo™-Tox HT

  • Fresh
  • Cryopreserved
  • Male
  • Female
  • Individual or pooled
  • ES- and/or iPS-derived
  • Hepatocyte cell lines e.g. HepG2
 Species Available for Use with HepatoGlo™-Tox HT

  • Human
  • Non-human primate
  • Dog
  • Rat
  • Mouse
 Assays that can be Multiplexed with HepatoGlo™-Tox HT
  • Cytochrome P450 enzyme assays.
  • Cellular drug-drug interaction assays.
  • Membrane integrity: LDH or PI dye exclusion.
  • Apoptosis: Biochemical caspase detection.
  • Mitochondrial dysfunction: Mitochondrial ToxGlo™
  • Glutathione Assay (GSH): Oxidative stress.
  • OxyFLOW™: Oxidative DNA damage.