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Nanoparticle Toxicity Panel

 

Nanotoxicity Testing Panel performed by HemoGenix

 

 

 

NCL Recommended In Vitro Nanoparticle Characterization Assays and Alternatives
Recommended Assay NCL Protocol No. Alternative Assay
Mouse granulocyte-macrophage (GM) colony-forming cell (CFU) assay ITA-3 HALO®-Tox HT and/or HALO®-Tox Real Time (RT) for multiple cell populations and species
Leukocyte proliferation assay ITA-6 ImmunoGlo-Tox HT from different tissues and species
Nitric oxide (NO) production by RAW264.7 macrphage cell line ITA-7 NO production from macrophage cell lines and primary macrophages

Chemotaxis assay using the human

HL-60 cell line

ITA-8 Calcein-AM real-time fluorescence chemotaxis using HL-60 and primary human peripheral blood or bone marrow cells
Zymosan phagocytosis assay using luminol on the HL-60 cell line ITA-9 Same, but including primary human peripheral blood or bone marrow cells
Analysis of IL-8, IL-1b, TNF alpha, IGN gamma by normal human peripheral blood mononuclear cells by ELISA ITA-10, 22, 23, 24, 25 Same, but including bone marrow

Effects on peripheral blood monocyte-derived dendritic cell maturation using flow cytometry

ITA-14 Same

Oxidative stress on Hep-G2 hepatocyte using the glutathione assay

GTA-3 Same, but using also primary hepatocytes and/or other cells

Lipid peroxidation assay on Hep-G2 cells

GTA-4

Same, but using also primary hepatocytes and/or other cells
Reactive oxygen species (ROS) assay on Hep-G2 hepatocytes GTA-7

Similar, but using also primary hepatocytes and/or other cells

Kidney cytotoxicty assay using MTT and LDH assays on LLC-PK1 kidney cells

GTA-1 STEMGlo-Tox HT and LDH fluorescence assays using kidney cell lines or primary kidney cells

Hep-G2 hepatocyte cytotoxicity assay using MTT and LDH assays

GTA-2 HepatoGlo-Tox HT and LDH fluorescence assays using Hep-G2 and primary hepatocytes
Kidney apoptosis assay for caspase 3 using LLC-PK1 cells  GTA-5 Similar, but also using primary kidney cells

Caspase 3  and 3/7 apoptosis assays on Hep-G2 cells

GTA-6, GTA-14 Simular, but also using primary hepatocytes. Luminescence caspase 3/7 assay

 

For more information and HemoGenix capabilities, please contact HemoGenix.