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Nanoparticle Toxicity Panel

 

Nanotoxicity Testing Panel performed by HemoGenix

 

 

Nanparticles are now used in a multitude of different products. There potential toxicity became apparent some years ago with the establishment of a special nanotoxicity laboratory within the National Cancer Institute (NCI). Nanotoxicity can be assessed at different levels, including the in vitro level. Many of the recommended in vitro tests for nanoparticles no have far superior alternatives, many of which only HemoGenix® can provide. 

 

For more information on nanotoxicity services, please contact HemoGenix® at info@hemogenix.com or call (719) 264-6250. These services are also available through Science Exchange.

 

NCL Recommended In Vitro Nanoparticle Characterization Assays and Alternatives
Recommended Assay NCL Protocol No. Alternative Assay
Mouse granulocyte-macrophage (GM) colony-forming cell (CFU) assay ITA-3 HALO®-Tox HT and/or HALO®-Tox Real Time (RT) for multiple cell populations and species
Leukocyte proliferation assay ITA-6 ImmunoGlo-Tox HT from different tissues and species
Nitric oxide (NO) production by RAW264.7 macrphage cell line ITA-7 NO production from macrophage cell lines and primary macrophages

Chemotaxis assay using the human

HL-60 cell line

ITA-8 Calcein-AM real-time fluorescence chemotaxis using HL-60 and primary human peripheral blood or bone marrow cells
Zymosan phagocytosis assay using luminol on the HL-60 cell line ITA-9 Same, but including primary human peripheral blood or bone marrow cells
Analysis of IL-8, IL-1b, TNF alpha, IGN gamma by normal human peripheral blood mononuclear cells by ELISA ITA-10, 22, 23, 24, 25 Same, but including bone marrow

Effects on peripheral blood monocyte-derived dendritic cell maturation using flow cytometry

ITA-14 Same

Oxidative stress on Hep-G2 hepatocyte using the glutathione assay

GTA-3 Same, but using also primary hepatocytes and/or other cells

Lipid peroxidation assay on Hep-G2 cells

GTA-4

Same, but using also primary hepatocytes and/or other cells
Reactive oxygen species (ROS) assay on Hep-G2 hepatocytes GTA-7

Similar, but using also primary hepatocytes and/or other cells

Kidney cytotoxicty assay using MTT and LDH assays on LLC-PK1 kidney cells

GTA-1 STEMGlo-Tox HT and LDH fluorescence assays using kidney cell lines or primary kidney cells

Hep-G2 hepatocyte cytotoxicity assay using MTT and LDH assays

GTA-2 HepatoGlo-Tox HT and LDH fluorescence assays using Hep-G2 and primary hepatocytes
Kidney apoptosis assay for caspase 3 using LLC-PK1 cells  GTA-5 Similar, but also using primary kidney cells

Caspase 3  and 3/7 apoptosis assays on Hep-G2 cells

GTA-6, GTA-14 Simular, but also using primary hepatocytes. Luminescence caspase 3/7 assay

 

For more information and HemoGenix capabilities, please contact HemoGenix.