The CAMEO™ Platform
The CAMEO™ Platform from HemoGenix® consists of 2 different in vitro assays.
CAMEO™-4
CAMEO™-4 is a traditional colony-forming cell (CFC) assay that has been miniaturized so that 4 replicates of 0.1ml culture reagents are dispensed into a 4-well, 35mm Petri dish.
The total volume of the culture required for all 4 replicates is only 0.6ml. Unlike the traditional CFC assay for human cells, CAMEO™-4 using human target cells can be completed in just 7 days if required, rather than 14 days. This is because HemoGenix® determined in determined and showed in 2003 (Curr. Opin. Drug Disc. Develop. 6/1:100-109) that undifferentiated clusters or colonies of cells, designated "proliferation units" (PUs) counted at 7 days of incubation correlated not only with the proliferation of the cells, but also colonies of functionally mature cells counted at 10 or 14 days.
Click here to go to the CAMEO™-4 page.
CAMEO™-96
CAMEO™-96 is the only in vitro assay that allows the user to detect and measure both proliferation and differentiation of lympho-hematopoietic stem and progenitor cells in the same assay.
For this reason, the assay has been given the name of CAMEO™-96, although proliferation occurs prior to differentiation. CAMEO™-96 is performed in a 96-well plate and uses exactly the same reagents as CAMEO™-4. The assay is performed in 2 stages. The first stage involves counting colonies in each well in a similar manner to the traditional CFC assay. After counting the colonies manually to assess differentiation, the proliferation status of the cells is determined using an ATP-based, luciferin/luciferase bioluminescence technology that allows the intracellular ATP (iATP) concentration to be measured in a plate luminometer. The iATP concentration is directly proportional to the proliferation status of the cells. Thus, both differentiation and proliferation are determined using the same cell culture, but different readouts.
Click here to go to the CAMEO™-96 page.
To download a copy of the Summary of Assays & Kits for Lympho-Hematopoietic Cells shown below, click here.
