DNA Oxidative Damage

OxyFLOW

 

Oxidative DNA Damage

detected by

OxyFLOW™
in cell suspensions by flow cytometry

 

Oxidative DNA Damage
Free radical production and oxidative DNA damage have been associated with multiple organs and disease conditions including:

  • Aging
  • Arthritis
  • Teratogenesis
  • Stroke
  • Neurodegenerative disorders (Alzheimer’s disease)
  • Carcinogenesis
  • Anti-cancer drug therapy
  • Crohn’s disease
  • Multiple sclerosis.

 

Applications
Oxidative injury to macromolecules is implicated in a wide range of pathological conditions. Damage is mediated via free radicals that can be caused by a number of agents. The OxyFLOW™ Kit can therefore be used in a number of applications.

  • Genotoxicity
  • Xenobiotics
  • Oxidative stress / Nutrient deficiency
  • Radiation biology
  • Inflammation
  • Ischemia-reperfusion injury
  • Effectiveness of cosmetics and sunscreen protection
  • Normal metabolic activity
  • Survival and protection by growth factors


Free radicals can react with DNA leading to mutation, carcinogenesis and cell death. All proliferating cells are susceptible to this type of damage and stem cells lead the list because they have the greatest proliferative potential. Damage to the stem cells results in dramatic perturbation of normal steady-state conditions and can lead to malignancy.

 

The OxyFLOW™ Platform
OxyFLOW™ is an assay that detects oxidative DNA damage in suspensions of mammalian cells as a result of stress, xenobiotics and the administration of drugs. Reactive oxygen species (ROS) produced under these conditions are particularly damaging to DNA. The result of oxidative DNA damage is the formation of 8-oxoguanine adducts.

OxyFLOW™ utilizes a proprietary fluorochrome (FITC)-conjugated 8-oxoguanine binding protein in a simple flow cytometric tube assay. Since OxyFLOW™ uses only 1 channel of the flow cytometer, it is possible to incubate the cells with additional antibodies or other membrane or intracellular markers conjugated to different fluorochromes in order to define the cell population exhibiting oxidative DNA damage.