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White Papers

HemoGenix™ White Papers

Please navigate to the Preferred Cell Systems Website

 

50 Years of Colony-Forming Assays for Hematopoietic Stem and Progenitor Cells: A Comparison and the Future

December 2016. Download the Application Note.

 

"Stem Cells and Predictive In Vitro Toxicity Testing".

November, 2011. Download the White Paper here.

 

Stem Cell Potency White Papers 

October 2007: Cell Potency Assays for the 21st Century Stem Cell Transplantation and Cord Blood Storage Processing Laboratories.

 

February 2009: Measurement of Hematopoietic Stem Cell Potency Prior to Transplantation

 

October 2010: The Science and Biology of Hematopoietic Stem Cell Potency, Quality and Release Criteria for Transplantation

 

CFU and HALO Cost-Analysis White Paper

April 2010: A Cost-Analysis of Colony-Forming Assays and Equivalent Alternatives used to Determine Growth of Hematopoietic Cells Derived from Umbilical Cord Blood, Normal and Mobilized Peripheral Blood and Bone Marrow.

Posters

Posters

"A Cord Blood Potency Assay that Accurately Predicts Engraftment Potenial", presented at the 2014 Cord Blood Symposium, San Francisco, CA, June 5-7.

 

"Assay Validation: How a CFU Replacement Assay is Validated for Cord Blood, Mobilized Peripheral Blood and Bone Marrow" AABB, Boston, MA. 2012. PDF File 8MB.

 

"A Potency Assay for Mobilized Peripheral Blood, Umbilical Cord Blood and Bone Marrow Stem Cell Products"

AABB, San Diego, CA 2011. PDF File >22MB

"A Potency Assay for Mobilized Peripheral Blood, Umbilical Cord Blood and Bone Marrow Stem Cell Products"
AABB, San Diego, CA 2011. JPEG File 4.6MB

 

"A Stem Cell Potency and Release Criteria Assay Specifically Designed for Umbilical Cord BloodCord Transplantation that is Compliant With Regulatory Guidelines"  presented at the International Society of Cellular Therapy (ISCT), San Diego, CA 2009

 

"A Novel Stem, Cell Potency and Release Assay for Umbilical Cord Blood: Correlation of Stem Cell Proliferation with Engraftment" presented at the International Society of Cellular Therapy (ISCT), May 2008 in Miami, FL 

 

"Proliferation Assays Provide Non-Subjective and Standardized Alternatives for Assessing the Potency of Stem Cell Products for Storage and Transplantation" presented at the International Society of Cellular Therapy (ISCT), Sydney, Australia, June 2007

 

"A Novel Assay to Evaluate the Functional Potential of Umbilical Cord Blood Progenitor" presented at the International Society of Cellular Therapy (ISCT), Sydney, Australia,June 2007

 

"Erythropoietin Protects Cells from Oxidative Stress by Reducing Oxidative DNA Damage" presented at the Society of Toxicology Meeting in Charlotte, North Carolina in March 2007

Articles

Articles and Links

Please navigate to the Preferred Cell Systems Website

A Standardized Protocol to Assess Stem Cell Potency. Steffen Porwolik. SamplingScience Blog. January 29, 2015. Link: http://www.biocision.com/blog/11485/assessing-stem-cell-potency. Download article. 

 

Predicting Organ Toxicity In Vitro: Bone Marrow. Ivan N. Rich and Adrew J. Olaharski. In: Drug Discovery Toxicology: From Target Assessment to Translational Biomarkers. 1st Edition. Eds. Yvonne Will, J Eric McDuffie, Andrew J. Olaharski and Branson D. Jeffy. Jon Wiley & Sons. (2016).

 

Bioluminescence Potency Measurement opf Cellular Therapy Products. Hilli Harper and Ivan N. Rich. In: Cellular Therapy: Principles, Methods, and Regulations. 2nd Edition. Eds. Ellen Areman and Kathy Loper. AABB (2016).

 

Measuring Stem Cell Circadian Rhythm. William Hrushesky and Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015). 

 

Measuring the Potency of a Stem Cell Therapeutic. Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).

 

Measurement of Hematopoietic Stem Cell Proliferation, Self-Renewal and Expansion Potential. Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).

 

A Short Primer in Stem Cell Biology. Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).

 

Improving Quality and Potency Testing for Umbilical Cord Blood: A New Perspective, by Ivan N. Rich. Stem Cells Translational Medicine. First published on July 9, 2015; DOI 10.5966/sctm.2015-0036. Available at: http://stemcellstm.alphamedpress.org/content/early/2015/07/09/sctm.2015-0036.full.pdf+html

 

Detecting Primitive Hematopoietic Stem Cells in Total Nucleated and Mononuclear Cell Fractions from Umbilical Cord Blood Segments and Units, by John Patterson, Calli H. Moore, Emily Palser, Jason C. Hearn, Daniela Dumitru, Holli A. Harper and Ivan N. Rich. Journal of Translational Medicine (2015) 13:94. DOI 10.1186/s12967-015-0434-z. Available at: http://www.translational-medicine.com/content/13/1/94.

 

Present Cord Blood Testing Fails to Determine if the Stem Cells Used for Transplantation are of High Quality and Potency, by Ivan N. Rich. On The Parents Guide to Cord Blood Foundation wbsite. Available at: http://parentsguidecordblood.org/en/news/present-cord-blood-testing-fails-determine-if-stem-cells-used-transplantation-are-high-quality

 

Potency, Proliferation and Engraftment Potential of Stem Cell Therapeutics: The Relationship between Potency and Clinical Outcome for Hematopoietic Stem Cell Products. Published as an Open Access review article in J. Cell Science & Therapy (2013, S13).

The Difference Between Stem Cell Viability and Potency: A Short Guide for Parent and Patients, by Ivan N. Rich. On The Parents Guide to Cord Blood Foundation website. Available at: http://parentsguidecordblood.org/en/news/difference-between-stem-cell-viability-and-potency-short-guide-parents-and-patients.

 

Stem Cell Predictive Hemotoxicology, by Holli Harper and Ivan Rich, (2013). Stem Cell Predictive Hemotoxicology, Stem Cell Biology in Normal Life and Diseases, Prof. Kamran Alimoghaddam (Ed.), ISBN: 978-953-51-1107-8, InTech, Available from: http://www.intechopen.com/books/stem-cell-biology-in-normal-life-and-diseases/stem-cell-predictive-hemotoxicology.

 

Hematopoietic Stem Cell Potency for Cellular Therapeutic Transplantation, by Hall KM, Harper H, & Rich IN. In: Hematopoietic Stem Cell Research,

Full reference: Karen M. Hall, Holli Harper and Ivan N. Rich (2012). Hematopoietic Stem Cell Potency for Cellular Therapeutic Transplantation, Advances in Hematopoietic Stem Cell Research, Rosana Pelayo (Ed.), ISBN: 978-953-307-930-1, InTech, Available from: http://www.intechopen.com/articles/show/title/hematopoietic-stem-cell-potency-for-cellular-therapeutic-transplantation

This is the first definitive article on how hematopoietic stem cell potency is measured.

 

Cellular Therapy: Principles, Methods and Regulations. Eds. Areman EM, Loper K. 2009 pp. 581-591

"Bioluminescence Assays for Assessing Potency of Cellular Therapy Products".

A article describing how cord blood potency is measured using HALO®-96 PQR. 

 

Toxicology Letters, 2009 188/2: 98-103

"In vitro to in vivo concordance of a high throughput assay of bone marrow toxicity across a diverse set of drug candidates".

Demonstrates that HALO® provides greater than 80% concordance with in vivo results. 

 

Current Opinion in Drug Discovery and Development, 2003 Vol. 6/1:100-109

"In vitro hematotoxicity testing in drug development: A review of past, present and future applications".

Demonstrates how HALO® correlates with the colony-forming cell (CFC) assay and why HALO can be performed more rapidly than the CFC assay.

 

Expert Opinion in Drug Metabolism and Toxicology, 2007 Vol.3, pp 295-307.

"High-throughput in vitro hemotoxicity testing and in vitro cross-platform comparative toxicity".

This article describes the drawbacks of the colony-forming assay technique in hemotoxicity testing, development and descriptions of the HALO® Platforms, validation of the HALO® platforms against the colony-forming assay, predicting hemotoxicity and the ComparaTOX™ Platform for in vitro cross-platform comparative toxicity testing. Please contact HemoGenix®.

 

Toxicological Sciences 2005 Vol. 87/2, pp 427-441.

"Validation and Development of a Predictive Paradigm for Hemotoxicology using a Multifunctional Bioluminescence Colony-Forming Proliferation Assay".

 

Journal of Hematotherapy 1997 Vol. 6, pp 191-192

"Standardization of the CFU-GM Assay using Hematopoietic Growth Factors".

 

Mutation Research, 2007 Vol. 625, pp 72-82.

"Diesel exhaust influences carcinogenic PAH-induced genotoxicity and gene expression in human breast epithelial cells in culture" by L.A.Couter, C. Pereira and W.M. Baird.

This articles uses the OxyFLOW™ Platform to measure oxidative DNA damage.