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Step by Step Protocols

Step by Step Protocols

 

The following protocols show the individual steps for performing an assay. They are available as a Quicktime move or as a PDF file. Please note that the latter are large files and may take some time to download.

 

HALO®-96 SPC-QC Step by Step Protocol

For the Quicktime movie format, please click here.

For the PDF file, please click here.

 

HALO®-96 PQR Step by Step Protocol

For the Quicktime movie format, please click here.

For the PDF format, please click here.

Presentations and Webinars

Presentations and Webinars

 

"Does Cord Blood Potency Correlate with Clinical Outcome?" Webinar on October 23, 2012 By Ivan N. Rich, PhD, CEO of HemoGenix. To view this recorded webinar, you must have been a registered participant. If you registered for this webinar, but were unable to attend, please send an email to info@hemogenix.com.

 

Mesenchymal Stem / Stromal Cell Webinar presented on July 13, 2011 by Ivan N. Rich, PhD, CEO of HemoGenix and James Musick, PhD, CEO of Vitro Biopharma. This webinar was not recorded. Only the slides are provided. This presentation is 2.7MB large and may take a few minutes to download.

Product Information Flyers

Product & Service Information

 

A Comparison Between Assays from HemoGenix and Other Suppliers

Availability of Assays for Hematopoietic Cells from HemoGenix® and Other Suppliers 

 

Tools for Life Sciences

Tools for Studying Mesenchymal Stem Cells (MSC) 

 

Contract Services

Summary of Contract Services

Stem Cell Characterization

Stem Cell Therapy Services

Stem Cell Potency Services

Stem Cell Micronucleus Assay

Hemotoxicity & Blood System Services

Immunotoxicity & Immune System Services

MSC Toxicity and MSC System Services

Hepatotoxicity Contract Services

Neurotoxicity Services

Nanoparticle Toxicity Services

Oxidative DNA Damage and Toxicity Services

ComparaTOX™ Contract Services

 

General Information

Proliferation and Differentiation

CFU and HALO: The Facts

Benfits of using HALO Assays in Cord Blood Blood Stem Cell Therapy

Summary of Hematopoietic Stem Cell Therapy Assays

 

Culture and Growth Media

HemoGro™ - Serum-free medium for hematopoietic cells

CellExpand™ - Hematopoietic Cell Expansion Medium

CRUXRUFA™ Human Platelet Lysate

MSCGro™ - Growth and differentiation media for mesenchymal stem/progenitor cells

  

Methylcellulose-Based Colony-Forming Unit (CFU) Assays for

Lympho-Hematopoietic Cells 

ColonyGro™ - 35mm Petri dish methylcellulose-based CFU Assay

CAMEO-4™ - Miniaturized methylcellulose-based CFU Assay

CAMEO-4™  - Miniaturized methylcellulose-based CFU assays for cell processing laboratories

CAMEO-96™ - Stanadrdized methylcellulose-based CFU proliferation and differentiation assays

 

Real Time Bioluminescence Proliferation and Viability Assays

HALO®-Real Time for lympho-hematopoietic stem and progenitor cells

MSCGlo™-Real Time for mesenchymal stem/progenitor cells (MSC/MPC)

ImmunoGlo™-Real Time for immune cells

 

ATP Bioluminescence Viability, Proliferation, Cytotoxicity and Functionality Assays

LIVEGlo™ - Metabolic viability assay for any mammalian cell type

HALO®-96 Research - Human and animal stem and progenitor lympho-hematopoietic cell assays

HALO®-96 PREP - Human and animal stem cell self-renewal and expansion assays

HALO®-96 PCAEQ - Human hematopoietic stem and progenitor cell CFU-equivalent assays

STEMpredict™ - Human predictive stem cell assay fo cord blood banking and mobilized peripheral blood

HALO®-96 SPC-QC - Human quality control assays for stem cell manufacturing and processing

HALO®-Potency - Human potency, quality and release assay for stem cell products

HALO®-96 TE - Time to hematopoietic engraftment after transplantation assays

HALO®-96 PMT - "Global" patient monitoring after stem cell transplantation assay

HALO®-Tox HT - Stem and progenitor cell hemotoxicity assays in 96- or 384-well plate format

HALO®-96 PRT - Stem cell predictive residual toxicity assays

ImmunoGlo™-96 - Immune cell proliferation and function assay

ImmunoGlo™-96 TCP - T-lymphocyte proliferation assays

ImmunoGlo™-MLC - Mixed lymphocyte culture/reaction assay

MSCGlo™-96 Research - Mesenchymal stem/progenitor cell (MSC) assays

MSCGlo™-96 HuQC - Human quality control assays for MSC manufacturing and processing

MSCGlo™-Potency - Human potency, quality and release assay for MSC products

MSCGlo™-Tox HT - MSC toxicity assays in 96- and 384-wll plate format

STEMGlo™-96 Research - Assay for ES, iPS and other cell lines and primary explanted cells

STEMGlo™-96 PREP - Human and animal stem cell self-rewal and expansion assay.

STEMGlo™-Potency - Human potency, quality and release assay for ES, iPS and primary explanted cells.

STEMGlo™-Tox HT - Toxicity assays for ES, iPS, cell lines and primated explanted cells in 96- or 384-well plate format

 

Fluorescence Viability, Proliferation and Functionality Assays

HemoFLUOR™-96 Research - Human and animal stem and progenitor lympho-hematopoietic cell assays

HemoFLUOR™-96 PCAEQ - Human hematopoietic stem and progenitor cell CFU-equivalent assays

HemoFLUOR-96 TE - Time to hematopoietic engraftment after transplantation assays

HemoFLUOR™-96 PMT - "Global" patient monitoring after stem cell transplantation assay

ImmunoFluor™-96 - Immune cell proliferation and function assay

ImmunoFluor™-MLC - Mixed lymphocyte culture/reaction assay

MSCFluor™-96 Research - Mesenchymal stem/progenitor cell (MSC) assays

STEMFluor™-96 Research - Assay for ES, iPS and other cell lines and primary explanted cells

 

Absorbance Lympho-Hematopoietic Proliferation Assays

HemoLIGHT™-96 Research - Human and animal stem and progenitor lympho-hematopoietic cell assays

HemoLIGHT™-96 PCAEQ - Human hematopoietic stem and progenitor cell CFU-equivalent assays

HemoLIGHT™-96 TE - Time to hematopoietic engraftment after transplantation assays

HemoLIGHT™-96 PMT - "Global" patient monitoring after stem cell transplantation assay

ImmunoLIGHT™-96 - Immune cell proliferation and function assay

ImmunoLIGHT™-MLC - Mixed lymphocyte culture/reaction assay

MSCLight™-96 - Mesenchymal stem/progenitor cell (MSC) assays

STEMLight™-96 Research - Assay for ES, iPS and other cell lines and primary explanted cells

 

Posters

Posters

"A Cord Blood Potency Assay that Accurately Predicts Engraftment Potenial", presented at the 2014 Cord Blood Symposium, San Francisco, CA, June 5-7.

 

"Assay Validation: How a CFU Replacement Assay is Validated for Cord Blood, Mobilized Peripheral Blood and Bone Marrow" AABB, Boston, MA. 2012. PDF File 8MB.

 

"A Potency Assay for Mobilized Peripheral Blood, Umbilical Cord Blood and Bone Marrow Stem Cell Products"

AABB, San Diego, CA 2011. PDF File >22MB

"A Potency Assay for Mobilized Peripheral Blood, Umbilical Cord Blood and Bone Marrow Stem Cell Products"
AABB, San Diego, CA 2011. JPEG File 4.6MB

 

"A Stem Cell Potency and Release Criteria Assay Specifically Designed for Umbilical Cord BloodCord Transplantation that is Compliant With Regulatory Guidelines"  presented at the International Society of Cellular Therapy (ISCT), San Diego, CA 2009

 

"A Novel Stem, Cell Potency and Release Assay for Umbilical Cord Blood: Correlation of Stem Cell Proliferation with Engraftment" presented at the International Society of Cellular Therapy (ISCT), May 2008 in Miami, FL 

 

"Proliferation Assays Provide Non-Subjective and Standardized Alternatives for Assessing the Potency of Stem Cell Products for Storage and Transplantation" presented at the International Society of Cellular Therapy (ISCT), Sydney, Australia, June 2007

 

"A Novel Assay to Evaluate the Functional Potential of Umbilical Cord Blood Progenitor" presented at the International Society of Cellular Therapy (ISCT), Sydney, Australia,June 2007

 

"Erythropoietin Protects Cells from Oxidative Stress by Reducing Oxidative DNA Damage" presented at the Society of Toxicology Meeting in Charlotte, North Carolina in March 2007

Articles

Articles and Links

A Standardized Protocol to Assess Stem Cell Potency. Steffen Porwolik. SamplingScience Blog. January 29, 2015. Link: http://www.biocision.com/blog/11485/assessing-stem-cell-potency. Download article

 

Predicting Organ Toxicity In Vitro: Bone Marrow. Ivan N. Rich and Adrew J. Olaharski. In: Drug Discovery Toxicology: From Target Assessment to Translational Biomarkers. 1st Edition. Eds. Yvonne Will, J Eric McDuffie, Andrew J. Olaharski and Branson D. Jeffy. Jon Wiley & Sons. (2016).

 

Bioluminescence Potency Measurement opf Cellular Therapy Products. Hilli Harper and Ivan N. Rich. In: Cellular Therapy: Principles, Methods, and Regulations. 2nd Edition. Eds. Ellen Areman and Kathy Loper. AABB (2016).

 

Measuring Stem Cell Circadian Rhythm. William Hrushesky and Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015). 

 

Measuring the Potency of a Stem Cell Therapeutic. Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).

 

Measurement of Hematopoietic Stem Cell Proliferation, Self-Renewal and Expansion Potential. Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).

 

A Short Primer in Stem Cell Biology. Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).

 

Improving Quality and Potency Testing for Umbilical Cord Blood: A New Perspective, by Ivan N. Rich. Stem Cells Translational Medicine. First published on July 9, 2015; DOI 10.5966/sctm.2015-0036. Available at: http://stemcellstm.alphamedpress.org/content/early/2015/07/09/sctm.2015-0036.full.pdf+html

 

Detecting Primitive Hematopoietic Stem Cells in Total Nucleated and Mononuclear Cell Fractions from Umbilical Cord Blood Segments and Units, by John Patterson, Calli H. Moore, Emily Palser, Jason C. Hearn, Daniela Dumitru, Holli A. Harper and Ivan N. Rich. Journal of Translational Medicine (2015) 13:94. DOI 10.1186/s12967-015-0434-z. Available at: http://www.translational-medicine.com/content/13/1/94.

 

Present Cord Blood Testing Fails to Determine if the Stem Cells Used for Transplantation are of High Quality and Potency, by Ivan N. Rich. On The Parents Guide to Cord Blood Foundation wbsite. Available at: http://parentsguidecordblood.org/en/news/present-cord-blood-testing-fails-determine-if-stem-cells-used-transplantation-are-high-quality

 

Potency, Proliferation and Engraftment Potential of Stem Cell Therapeutics: The Relationship between Potency and Clinical Outcome for Hematopoietic Stem Cell Products. Published as an Open Access review article in J. Cell Science & Therapy (2013, S13).

The Difference Between Stem Cell Viability and Potency: A Short Guide for Parent and Patients, by Ivan N. Rich. On The Parents Guide to Cord Blood Foundation website. Available at: http://parentsguidecordblood.org/en/news/difference-between-stem-cell-viability-and-potency-short-guide-parents-and-patients.

 

Stem Cell Predictive Hemotoxicology, by Holli Harper and Ivan Rich, (2013). Stem Cell Predictive Hemotoxicology, Stem Cell Biology in Normal Life and Diseases, Prof. Kamran Alimoghaddam (Ed.), ISBN: 978-953-51-1107-8, InTech, Available from: http://www.intechopen.com/books/stem-cell-biology-in-normal-life-and-diseases/stem-cell-predictive-hemotoxicology.

 

Hematopoietic Stem Cell Potency for Cellular Therapeutic Transplantation, by Hall KM, Harper H, & Rich IN. In: Hematopoietic Stem Cell Research,

Full reference: Karen M. Hall, Holli Harper and Ivan N. Rich (2012). Hematopoietic Stem Cell Potency for Cellular Therapeutic Transplantation, Advances in Hematopoietic Stem Cell Research, Rosana Pelayo (Ed.), ISBN: 978-953-307-930-1, InTech, Available from: http://www.intechopen.com/articles/show/title/hematopoietic-stem-cell-potency-for-cellular-therapeutic-transplantation

This is the first definitive article on how hematopoietic stem cell potency is measured.

 

Cellular Therapy: Principles, Methods and Regulations. Eds. Areman EM, Loper K. 2009 pp. 581-591

"Bioluminescence Assays for Assessing Potency of Cellular Therapy Products".

A article describing how cord blood potency is measured using HALO®-96 PQR. 

 

Toxicology Letters, 2009 188/2: 98-103

"In vitro to in vivo concordance of a high throughput assay of bone marrow toxicity across a diverse set of drug candidates".

Demonstrates that HALO® provides greater than 80% concordance with in vivo results. 

 

Current Opinion in Drug Discovery and Development, 2003 Vol. 6/1:100-109

"In vitro hematotoxicity testing in drug development: A review of past, present and future applications".

Demonstrates how HALO® correlates with the colony-forming cell (CFC) assay and why HALO can be performed more rapidly than the CFC assay.

 

Expert Opinion in Drug Metabolism and Toxicology, 2007 Vol.3, pp 295-307.

"High-throughput in vitro hemotoxicity testing and in vitro cross-platform comparative toxicity".

This article describes the drawbacks of the colony-forming assay technique in hemotoxicity testing, development and descriptions of the HALO® Platforms, validation of the HALO® platforms against the colony-forming assay, predicting hemotoxicity and the ComparaTOX™ Platform for in vitro cross-platform comparative toxicity testing. Please contact HemoGenix®.

 

Toxicological Sciences 2005 Vol. 87/2, pp 427-441.

"Validation and Development of a Predictive Paradigm for Hemotoxicology using a Multifunctional Bioluminescence Colony-Forming Proliferation Assay".

 

Journal of Hematotherapy 1997 Vol. 6, pp 191-192

"Standardization of the CFU-GM Assay using Hematopoietic Growth Factors".

 

Mutation Research, 2007 Vol. 625, pp 72-82.

"Diesel exhaust influences carcinogenic PAH-induced genotoxicity and gene expression in human breast epithelial cells in culture" by L.A.Couter, C. Pereira and W.M. Baird.

This articles uses the OxyFLOW™ Platform to measure oxidative DNA damage.