Catalogs of Products
Assay Kit and Reagent Catalog
For up to date pricing, please see our online catalog. Click here.
Tissue Culture and Specialized Microscope Accessory Products
Background and Technical Resources
Step by Step Protocols
The following protocols show the individual steps for performing an assay. They are available as a Quicktime move or as a PDF file. Please note that the latter are large files and may take some time to download.
HALO®-96 SPC-QC Step by Step Protocol
For the Quicktime movie format, please click here.
For the PDF file, please click here.
HALO®-96 PQR Step by Step Protocol
For the Quicktime movie format, please click here.
For the PDF format, please click here.
HemoGenix™ White Papers
50 Years of Colony-Forming Assays for Hematopoietic Stem and Progenitor Cells: A Comparison and the Future
December 2016. Download the Application Note.
"Stem Cells and Predictive In Vitro Toxicity Testing".
November, 2011. Download the White Paper here.
Stem Cell Potency White Papers
CFU and HALO Cost-Analysis White Paper
April 2010: A Cost-Analysis of Colony-Forming Assays and Equivalent Alternatives used to Determine Growth of Hematopoietic Cells Derived from Umbilical Cord Blood, Normal and Mobilized Peripheral Blood and Bone Marrow.
Presentations and Webinars
"Does Cord Blood Potency Correlate with Clinical Outcome?" Webinar on October 23, 2012 By Ivan N. Rich, PhD, CEO of HemoGenix. To view this recorded webinar, you must have been a registered participant. If you registered for this webinar, but were unable to attend, please send an email to email@example.com.
Mesenchymal Stem / Stromal Cell Webinar presented on July 13, 2011 by Ivan N. Rich, PhD, CEO of HemoGenix and James Musick, PhD, CEO of Vitro Biopharma. This webinar was not recorded. Only the slides are provided. This presentation is 2.7MB large and may take a few minutes to download.
Product & Service Information
A Comparison Between Assays from HemoGenix and Other Suppliers
Tools for Life Sciences
Culture and Growth Media
Methylcellulose-Based Colony-Forming Unit (CFU) Assays for
Real Time Bioluminescence Proliferation and Viability Assays
ImmunoGlo™-Real Time for immune cells
ATP Bioluminescence Viability, Proliferation, Cytotoxicity and Functionality Assays
LIVEGlo™ - Metabolic viability assay for any mammalian cell type
HALO®-96 Research - Human and animal stem and progenitor lympho-hematopoietic cell assays
HALO®-96 PREP - Human and animal stem cell self-renewal and expansion assays
HALO®-96 PCAEQ - Human hematopoietic stem and progenitor cell CFU-equivalent assays
STEMpredict™ - Human predictive stem cell assay fo cord blood banking and mobilized peripheral blood
HALO®-96 SPC-QC - Human quality control assays for stem cell manufacturing and processing
HALO®-Potency - Human potency, quality and release assay for stem cell products
HALO®-96 TE - Time to hematopoietic engraftment after transplantation assays
HALO®-96 PMT - "Global" patient monitoring after stem cell transplantation assay
HALO®-Tox HT - Stem and progenitor cell hemotoxicity assays in 96- or 384-well plate format
HALO®-96 PRT - Stem cell predictive residual toxicity assays
ImmunoGlo™-96 - Immune cell proliferation and function assay
ImmunoGlo™-MLC - Mixed lymphocyte culture/reaction assay
MSCGlo™-96 Research - Mesenchymal stem/progenitor cell (MSC) assays
MSCGlo™-96 HuQC - Human quality control assays for MSC manufacturing and processing
MSCGlo™-Potency - Human potency, quality and release assay for MSC products
MSCGlo™-Tox HT - MSC toxicity assays in 96- and 384-wll plate format
STEMGlo™-96 Research - Assay for ES, iPS and other cell lines and primary explanted cells
STEMGlo™-96 PREP - Human and animal stem cell self-rewal and expansion assay.
STEMGlo™-Potency - Human potency, quality and release assay for ES, iPS and primary explanted cells.
STEMGlo™-Tox HT - Toxicity assays for ES, iPS, cell lines and primated explanted cells in 96- or 384-well plate format
Fluorescence Viability, Proliferation and Functionality Assays
HemoFLUOR™-96 Research - Human and animal stem and progenitor lympho-hematopoietic cell assays
HemoFLUOR-96 TE - Time to hematopoietic engraftment after transplantation assays
HemoFLUOR™-96 PMT - "Global" patient monitoring after stem cell transplantation assay
ImmunoFluor™-96 - Immune cell proliferation and function assay
ImmunoFluor™-MLC - Mixed lymphocyte culture/reaction assay
MSCFluor™-96 Research - Mesenchymal stem/progenitor cell (MSC) assays
STEMFluor™-96 Research - Assay for ES, iPS and other cell lines and primary explanted cells
Absorbance Lympho-Hematopoietic Proliferation Assays
HemoLIGHT™-96 Research - Human and animal stem and progenitor lympho-hematopoietic cell assays
HemoLIGHT™-96 PCAEQ - Human hematopoietic stem and progenitor cell CFU-equivalent assays
HemoLIGHT™-96 TE - Time to hematopoietic engraftment after transplantation assays
HemoLIGHT™-96 PMT - "Global" patient monitoring after stem cell transplantation assay
ImmunoLIGHT™-96 - Immune cell proliferation and function assay
ImmunoLIGHT™-MLC - Mixed lymphocyte culture/reaction assay
MSCLight™-96 - Mesenchymal stem/progenitor cell (MSC) assays
STEMLight™-96 Research - Assay for ES, iPS and other cell lines and primary explanted cells
All assay kit instruction manuals are in bookfold PDF files. It will be necessary to configure Adobe Acrobat to print in bookfold and double-sided.
HALO®-96 PQR Manual
"A Stem Cell Potency and Release Criteria Assay Specifically Designed for Umbilical Cord BloodCord Transplantation that is Compliant With Regulatory Guidelines" presented at the International Society of Cellular Therapy (ISCT), San Diego, CA 2009
"A Novel Stem, Cell Potency and Release Assay for Umbilical Cord Blood: Correlation of Stem Cell Proliferation with Engraftment" presented at the International Society of Cellular Therapy (ISCT), May 2008 in Miami, FL
"Proliferation Assays Provide Non-Subjective and Standardized Alternatives for Assessing the Potency of Stem Cell Products for Storage and Transplantation" presented at the International Society of Cellular Therapy (ISCT), Sydney, Australia, June 2007
"A Novel Assay to Evaluate the Functional Potential of Umbilical Cord Blood Progenitor" presented at the International Society of Cellular Therapy (ISCT), Sydney, Australia,June 2007
"Erythropoietin Protects Cells from Oxidative Stress by Reducing Oxidative DNA Damage" presented at the Society of Toxicology Meeting in Charlotte, North Carolina in March 2007
Articles and Links
A Standardized Protocol to Assess Stem Cell Potency. Steffen Porwolik. SamplingScience Blog. January 29, 2015. Link: http://www.biocision.com/blog/11485/assessing-stem-cell-potency. Download article.
Predicting Organ Toxicity In Vitro: Bone Marrow. Ivan N. Rich and Adrew J. Olaharski. In: Drug Discovery Toxicology: From Target Assessment to Translational Biomarkers. 1st Edition. Eds. Yvonne Will, J Eric McDuffie, Andrew J. Olaharski and Branson D. Jeffy. Jon Wiley & Sons. (2016).
Bioluminescence Potency Measurement opf Cellular Therapy Products. Hilli Harper and Ivan N. Rich. In: Cellular Therapy: Principles, Methods, and Regulations. 2nd Edition. Eds. Ellen Areman and Kathy Loper. AABB (2016).
Measuring Stem Cell Circadian Rhythm. William Hrushesky and Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).
Measuring the Potency of a Stem Cell Therapeutic. Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).
Measurement of Hematopoietic Stem Cell Proliferation, Self-Renewal and Expansion Potential. Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).
A Short Primer in Stem Cell Biology. Ivan N. Rich. In: Stem Cell Protocols, Ed: Ivan N. Rich. Human Press (2015).
Improving Quality and Potency Testing for Umbilical Cord Blood: A New Perspective, by Ivan N. Rich. Stem Cells Translational Medicine. First published on July 9, 2015; DOI 10.5966/sctm.2015-0036. Available at: http://stemcellstm.alphamedpress.org/content/early/2015/07/09/sctm.2015-0036.full.pdf+html
Detecting Primitive Hematopoietic Stem Cells in Total Nucleated and Mononuclear Cell Fractions from Umbilical Cord Blood Segments and Units, by John Patterson, Calli H. Moore, Emily Palser, Jason C. Hearn, Daniela Dumitru, Holli A. Harper and Ivan N. Rich. Journal of Translational Medicine (2015) 13:94. DOI 10.1186/s12967-015-0434-z. Available at: http://www.translational-medicine.com/content/13/1/94.
Present Cord Blood Testing Fails to Determine if the Stem Cells Used for Transplantation are of High Quality and Potency, by Ivan N. Rich. On The Parents Guide to Cord Blood Foundation wbsite. Available at: http://parentsguidecordblood.org/en/news/present-cord-blood-testing-fails-determine-if-stem-cells-used-transplantation-are-high-quality
Potency, Proliferation and Engraftment Potential of Stem Cell Therapeutics: The Relationship between Potency and Clinical Outcome for Hematopoietic Stem Cell Products. Published as an Open Access review article in J. Cell Science & Therapy (2013, S13).
The Difference Between Stem Cell Viability and Potency: A Short Guide for Parent and Patients, by Ivan N. Rich. On The Parents Guide to Cord Blood Foundation website. Available at: http://parentsguidecordblood.org/en/news/difference-between-stem-cell-viability-and-potency-short-guide-parents-and-patients.
Stem Cell Predictive Hemotoxicology, by Holli Harper and Ivan Rich, (2013). Stem Cell Predictive Hemotoxicology, Stem Cell Biology in Normal Life and Diseases, Prof. Kamran Alimoghaddam (Ed.), ISBN: 978-953-51-1107-8, InTech, Available from: http://www.intechopen.com/books/stem-cell-biology-in-normal-life-and-diseases/stem-cell-predictive-hemotoxicology.
Hematopoietic Stem Cell Potency for Cellular Therapeutic Transplantation, by Hall KM, Harper H, & Rich IN. In: Hematopoietic Stem Cell Research,
Full reference: Karen M. Hall, Holli Harper and Ivan N. Rich (2012). Hematopoietic Stem Cell Potency for Cellular Therapeutic Transplantation, Advances in Hematopoietic Stem Cell Research, Rosana Pelayo (Ed.), ISBN: 978-953-307-930-1, InTech, Available from: http://www.intechopen.com/articles/show/title/hematopoietic-stem-cell-potency-for-cellular-therapeutic-transplantation
This is the first definitive article on how hematopoietic stem cell potency is measured.
Cellular Therapy: Principles, Methods and Regulations. Eds. Areman EM, Loper K. 2009 pp. 581-591
"Bioluminescence Assays for Assessing Potency of Cellular Therapy Products".
A article describing how cord blood potency is measured using HALO®-96 PQR.
"In vitro to in vivo concordance of a high throughput assay of bone marrow toxicity across a diverse set of drug candidates".
Demonstrates that HALO® provides greater than 80% concordance with in vivo results.
"In vitro hematotoxicity testing in drug development: A review of past, present and future applications".
Demonstrates how HALO® correlates with the colony-forming cell (CFC) assay and why HALO can be performed more rapidly than the CFC assay.
"High-throughput in vitro hemotoxicity testing and in vitro cross-platform comparative toxicity".
This article describes the drawbacks of the colony-forming assay technique in hemotoxicity testing, development and descriptions of the HALO® Platforms, validation of the HALO® platforms against the colony-forming assay, predicting hemotoxicity and the ComparaTOX™ Platform for in vitro cross-platform comparative toxicity testing. Please contact HemoGenix®.
"Validation and Development of a Predictive Paradigm for Hemotoxicology using a Multifunctional Bioluminescence Colony-Forming Proliferation Assay".
"Standardization of the CFU-GM Assay using Hematopoietic Growth Factors".
"Diesel exhaust influences carcinogenic PAH-induced genotoxicity and gene expression in human breast epithelial cells in culture" by L.A.Couter, C. Pereira and W.M. Baird.
This articles uses the OxyFLOW™ Platform to measure oxidative DNA damage.