HALO® Platforms for Hemotoxicity Testing

 

HemoTox

HemoGenix® offers 3 different HALO® Hemotoxicity Platforms.

HALO®-96 MeC (Methyl Cellulose) is similar to the traditional colony-forming assay in that it allows cells to grow under clonal conditions, but instead of manually counting colonies, an instrument-based, ATP bioluminescence readout is performed. The assay uses a 96-well plate format, but should really only be used for a small number of samples because the viscous methyl cellulose has to be dispensed manually. The HALO®-96 MeC Hemotoxicity Platform can be used for pre-clinical animal studies and patient monitoring during clinical trials.

HALO®-96 SEC (Suspension Expansion Culture) does not use methyl cellulose and therefore the whole assay, from beginning to end can be performed under fully automated conditions. The HALO®-96 SEC Hemotoxicity Platform is easier to use at any stage of drug discovery and development, from screening to patient monitoring during clinical trials. It is also 2-3 times more sensitive than the HALO®-96 MeC Hemotoxicity Platform.

HALO®-384 HT (High-Throughput) is similar to the HALO®-96 SEC Hemotoxicity Platform, but uses a 384-well plate format. It was specifically designed for drug discovery screening at the ADME/Tox level and is even more sensitive than the HALO®-96 SEC Hemotoxicity Platform.

 

For in-house hemotoxicity testing, HemoGenix® offers a large number of assay kit options.

  • Assay kits are available as 1 plate sampler kits
  • Assay kits are also available to determine apoptosis
  • All assay kit platforms are available in 2 and 4 plate kits sizes as well as bulk quantities.
  • All assay kit platforms can be obtained for detecting:
    • HPP-SP (primitive stem cells)
    • CFC-GEMM (mature stem cells)
    • BFU-E (erythropoietic progenitor cells)
    • GM-CFC (granulocyte-macrophage progenitor cells)
    • G-CFC (granulocyte precursor cells)
    • M-CFC (macrophage precursor cells)
    • Mk-CFC (megakaryocyte progenitor cells)
    • T-CFC (T-lymphocyte progenitor cells)
    • B-CFC (B-lymphocyte progenitor cells) or any combination of these populations.
  • These populations can be obtained as combination kits allowing 2 or 4 populations to be measured side-by-side.
  • All assay kit platforms are available for:
    • Human
    • Non-Human Primate (Cyno and Rhesus)
    • Dog
    • Rat
    • Mouse and any combination of these.
  • 7-Population HALO® Predictive Hemotoxicity Testing is available for all three HALO® Platforms
  • The HALO® Predictive Residual Response (PRR) Hemotoxicity Kits are available for all species using HALO®-96 MeC and HALO®-96 SEC.
  • HemoGenix® can also provide custom kits, usually at no extra charge.


Hands-On Training

HemoGenix® offers a comprehensive 2-3 day training course. During that time, you will learn the background of performing hemotoxicity testing using the HALO® Platform, you will get hands-on experience at setting up a complete experiment as well as how to process the plates and evaluate and interpret the results. You will have all the necessary experience to begin using the HALO® and our other ATP-based bioluminescence platforms.

We invite you to either train at our facility in Colorado Springs, Colorado, or we can train your personnel at your own facility. Call us for more information.

 

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Compare different HALO® Platform formats

Which HALO® format is the right one for your application