There are now three HALO® Platforms:
The following table shows the important characteristics between the three HALO® Platforms and how they contrast with the traditional colony-forming assay. This table also provides information that will be important for you to choose a HALO® Platform suited to your specific application. This is also discussed in "Which HALO® Format is Right for You?"
| Property |
Colony-Forming Assay (CAMEO™-4) |
HALO®-96 MeC |
HALO®-96 SEC |
HALO®-384 HT |
| Type of assay: | Differentiation |
Proliferation / Cytotoxicity - |
Proliferation / Cytotoxicity - Apoptosis |
Proliferation / Cytotoxicity - Apoptosis |
| Validated |
No |
Yes |
Yes |
Yes |
| Type of culture: |
Methyl cellulose |
Methyl cellulose |
Suspension |
Suspension |
| Cell growth: |
Clonal |
Clonal |
Expansion |
Expansion |
| Parameter measured: |
Cell colonies |
Intracellular ATP |
Intracellular ATP | Intracellular ATP |
| Readout: |
Manual / Microscopy |
Luminescence / Instrument-based |
Luminescence / Instrument-based | Luminescence / Instrument-based |
| Subjectivity: |
Subjective |
Non-subjective |
Non-subjective |
Non-subjective |
| Calibration: |
Not standardized |
External ATP |
External ATP |
External ATP |
| Format |
35mm Petri dishes |
96-well plate |
96-well plate |
384-well plate |
| Assay description: |
Macro |
Mini |
Mini |
Micro |
| Volume of assay: |
1ml |
100µl |
100µl |
25µl |
| No. of replicates: |
2-3 |
Min. 4 - unlimited |
Min. 4 - unlimited | Min. 4 - unlimited |
| Cell incubation time: |
7-14 days |
5-7 days |
3-5 days |
3-5 days |
| Turnaround time: |
7-14 days |
5-7 days |
3-5 days |
3-5 days |
| Processing time / Readout time: |
None / ~10 min/dish | 20 min/plate / <5min/plate |
20 min/plate / <5min/plate | 20 min/plate / <5min/plate |
| Throughput capability: |
Low |
Medium |
Medium - High |
High |
| Training: |
6-12 months |
2 days |
2 days |
2 days |