There are now three HALO® Platforms:
The following table shows the important characteristics between the three HALO® Platforms and how they contrast with the traditional colony-forming assay. This table also provides information that will be important for you to choose a HALO® Platform suited to your specific application. This is also discussed in "Which HALO® Format is Right for You?"
| Property | Colony-Forming Assay (CAMEO™-4) | HALO®-96 MeC | HALO®-96 SEC | HALO®-384 HT |
| Type of assay: | Differentiation | Proliferation / Cytotoxicity - | Proliferation / Cytotoxicity - Apoptosis | Proliferation / Cytotoxicity - Apoptosis |
| Validated | No | Yes | Yes | Yes |
| Type of culture: | Methyl cellulose | Methyl cellulose | Suspension | Suspension |
| Cell growth: | Clonal | Clonal | Expansion | Expansion |
| Parameter measured: | Cell colonies | Intracellular ATP | Intracellular ATP | Intracellular ATP |
| Readout: | Manual / Microscopy | Luminescence / Instrument-based | Luminescence / Instrument-based | Luminescence / Instrument-based |
| Subjectivity: | Subjective | Non-subjective | Non-subjective | Non-subjective |
| Calibration: | Not standardized | External ATP | External ATP | External ATP |
| Format | 35mm Petri dishes | 96-well plate | 96-well plate | 384-well plate |
| Assay description: | Macro | Mini | Mini | Micro |
| Volume of assay: | 1ml | 100µl | 100µl | 25µl |
| No. of replicates: | 2-3 | Min. 4 - unlimited | Min. 4 - unlimited | Min. 4 - unlimited |
| Cell incubation time: | 7-14 days | 5-7 days | 4-6 days | 4-6 days |
| Turnaround time: | 7-14 days | 5-7 days | 4-6 days | 4-6 days |
| Processing time / Readout time: | None / ~10 min/dish | 20 min/plate / <5min/plate | 20 min/plate / <5min/plate | 20 min/plate / <5min/plate |
| Throughput capability: | Low | Medium | Medium - High | High |
| Training: | 6-12 months | 2 days | 2 days | 2 days |