LUMENESC™ vs CFC-F


LUMENESC

 

The following table shows a comparison between the manual CFU-F assay and the instrument-based LUMENESC™ Platform.

Property CFU-F
 		LUMENESC™
Type of assay:
Differentiation
Proliferation
Type of Culture:
Aderent / Suspension
Adherent / Suspension
Cell growth:
Expansion
Expansion
Parameter measured:
Stained cells
Intracellular ATP
Readout:
ManualBioluminescence
Readout type:
Microscope
Luminometer
Subjectivity:
Subjective
Non-subjective
Standardization:
None
External ATP standard
Format:
60-100mm Petri dishes
96- or 384-well plate
No. of replicates:
Very limited
Unlimited
Volume of assay:
Several mls100µl or 25µl
Incubation time:
14 days
10 days
Processing time:
<5 min/plate
30 min/96- or 384-well plate
Multiparameter capability:
None
Can be combined with phenotypic analysis and apoptosis detection
Throughput capability:
Low
Medium to high

HemoGenix® has developed 3 types of LUMENESC™ Assay Kits from its Contract Research Services

LUMENESC™-96 Research: Basic research involving MSC, CFC-F and studies on MSC differentiation. This kit provides the investigator with the flexibility to grow and treat the cells using their own protocols.


LUMENESC™-96 QC: For cell processing quality control for transplantation and cord blood storage. Designed to be used in conjunction with the HALO® SPC-QC Platform. Like the HALO® SPC-QC, the Master Mix is already prepared for the user in separate tubes for each sample. The cells are added to the tube, mixed and dispensed into the 96-well plates provided with the kit. The proliferative status of the cells is determined by processing the wells used and measuring the luminescence in a plate luminometer.


LUMENESC™-96 or 384-Tox: To determine the cytotoxic effect of agents on the MSC system. Cells are provided by the user, and aliquoted into the wells of a 96- or 384-well plate provided. After allowing the cells to adhere for 24 hours, the cells are allowed to enter the exponential growth phase. Prior to replacing the growth medium with the test compound, the proliferative status of growing cells in 4-8 replicate wells is determined. The growth medium in the rest of the wells is again replaced and the agent added (usually in a dose response manner). Cytotoxicity is determined after a period of culture by measuring the proliferative status of the cells using the reagents provided to measure luminescence.

 

 
Characteristics of the new, 3rd Generation Luminescence Reagent for LUMENESC™


Simple
  • "Add-Mix-and-Measure" format uses a single reagent incorporating both cell lysis and luminescence-generating reagents.
Increased Sensitivity
  • 2-3 times brighter signal means signficantly increased sensitivity and reduced cell concentration requirement.
Fast
  • Only 15 minutes to develop and measure results in a 96-well plate.
  • Cuts time in half compared to previous reagents and saves on supplies.
Reagent Stability
  • Significantly reduced decay times.
  • No need to measure luminescence immediately.
  • Improved shelf life.
  • Improved handling and shipping conditions.

 

 

LUMINOMETERS
A plate luminometer is not the same as that for measuring absorbence or fluorescence. Multi-parameter instruments are available that can measure, absorbence, fluorescence and luminescence. Alternatively, a dedicated luminometer that only detects luminescence (both chemi- and bioluminescence) can also be obtained.
At HemoGenix® we have tested a number of luminometers from different manufacturers. These include:
  • TECAN
  • Molecular Devices
  • BioTech
  • Bertold
Please call HemoGenix® at 719-264-6250 or e-mail HemoGenix® at info@hemogenix.com for advice and any other questions you might have regarding luminometers. HemoGenix® will also be marketing a luminometer in early 2007.

 

 

LINKS

Download the LUMENESC™-96 QC Protocol

Download the LUMENESC™-96 / 384 Tox Protocol