LumiSTEM™ like the HALO® and LUMENESC™ Platforms, utilize a highly sensitive ATP-based bioluminescence signal detection system. Detection of intracellular ATP actually allows 4 different parameters to be detected or implied. These are:
In addition to these ATP-dependent parameters, a great deal more information can be obtained from the LumiSTEM™ Platform, by simply increasing the number of replicate wells/sample. By increasing the number of wells initially prepared, multiple parameters can be obtained from the same sample. For instance, if instead of the normal number of wells prepared to detect proliferation or cytotoxicity, double the number of wells is set up, then half can be used for the proliferation/cytotoxicity assay, while the other half can be removed and used for phenotypic analysis, if the necessary antibodies are available. The supernatant from the individual or pooled cultures can be analyzed for growth factors, cytokines or other soluble factors using ELISAs etc. If more replicate wells are prepared, then the remainder can be used for gene expression analysis. In this way, an extremely large amount of information can be obtained from a single sample.
LumiSTEM™ is also part of the ComparaTOX™ Platform, a Predictive In Vitro, Cross-Platform Comparative Toxicity Testing system that combines LumiSTEM™ with LUMENESC™ to detect toxicity to the mesenchymal stem cell system, and HALO® to detect toxicity to the lympho-hematopoietic system.
LINKS
Application of the ComparaTOX™ Platform