Mechanism of Action - Apoptosis and Oxidative DNA Damage

LumiSTEM

When investigating the toxicity of agents to cells of the MSC system, it is often a prerequisite to also study the mechanism of action of these agents.

HemoGenix® has made it extremely easy to study whether agents induce apoptosis or oxidative DNA damage, which may also be a part of the apoptosis mechanism by instigating the stress pathways. The LumiSTEM™-96 adherent and non-adherent assays can incorporate apoptosis detection. By increasing the number of replicate wells performed for a single sample, a portion of the cells can be used to investigate whether oxidative DNA damage has occurred as a result of agent administration. Oxidative DNA damage is measured using the HemoGenix® OxyFLOW™ Flow Cytometry Platform.

 

For more information on these assays click on the links below.

LINKS

LumiSTEM™ - Apoptosis/Necrosis

OxyFLOW™