Mesenchymal Stem Cell System Research

LUMENESC

LUMENESC - A Colony-Forming Unit - Fibroblast (CFU-F) Alternative

Mesenchymal stem cells (MSC) or Mesenchymal Progenitor Cells (MPC) have the potential for producing multiple types of tissue including:

  • Hematopoietic stroma or supporting cells
  • Osteocytes
  • Chrondrocytes
  • Adipocytes
  • Skeletal, smooth and cardiac muscle
  • Astrocytes
  • Oligodendrocytes
  • Neurons

Mesenchymal stem cells constitute a proliferating cell system that can be detected and measured using the LUMENESC (Luminescence Measurement of Mesenchymal Stem Cells) Platform. LUMENESC uses the same instrument-based ATP bioluminescence signal detection system as HALO.

Hematopoietic supporting cells or stromal cells are recognized as a distinct heterogenous population during long-term culture of hematopoietic cells. However, the “hematopoietic inductive microenvironment”, postulated by Trentin (1971), is considered to consist of the hematopoietic stroma. In addition, the “stem cell niche”, first suggested by Schofield (1978) contains non-hematopoietic derived fibroblasts, endothelial cells, reticulum cells and fat cells, as well as macrophages. In accordance with the nomenclature used for hematopoietic stem, progenitor and precursor cells, a clonal population of fibroblastoid-like MSC or their immediate progeny was designated the colony-forming unit or cell - fibroblast (CFU-F or CFC-F) by Friedenstein and coworkers (1971). There are few, if any distinctive characteristics of MSC. Rather, several markers have been used that together, exclude other cell types and, in turn, define the MSC population.

There are several important MSC system research applications.
  • Analysis of individual cell types that make up the hematopoietic and non-hematopoietic microenvironment or stroma.
  • CFU-F assays.
  • Toxicity to the MSC system.
  • Cellular and genetic regulation of factors that result in the proliferation and differentiation of MSC into different cell types.
  • Transplantation ability and quality control of MSC.
  • Embryonic stem cell research.
  • Gene transfection studies
These and many more research applications can incorporate the LUMENESC Platform for fast, easy and standardized analysis of the MSC / CFC-F population and differentiating populations. If you have been growing CFC-F in Petri dishes and staining them in order to count under a microscope, the LUMENESC is the alternative. Using our protocols, MSC can be measured in 10 days.
 
LUMENESC is available in 1, 2, 3 and 4 plate kits. In addition, a kit is also available to detect apoptosis in the MSC System.