The OxyFLOW™ Platform
for detecting oxidative DNA damage in cell
suspensions by flow cytometry
The OxyFLOW™ Platform
OxyFLOW™ is an assay that detects oxidative DNA damage in suspensions of mammalian cells as a result of stress, xenobiotics and the administration of drugs. Reactive oxygen species (ROS) produced under these conditions are particularly damaging to DNA. The result of oxidative DNA damage is the formation of 8-oxoguanine adducts.
OxyFLOW™ utilizes a proprietary fluorochrome (FITC)-conjugated 8-oxoguanine binding protein in a simple flow cytometric tube assay. Since OxyFLOW™ uses only 1 channel of the flow cytometer, it is possible to incubate the cells with additional antibodies or other membrane or intracellular markers using different fluorochromes in order to define the cell population exhibiting oxidative DNA damage.