CLGlo™ and CLGlo™-HT

An ATP Bioluminomics™ Assay Platform for Cell Lines and Tumor Cells

   Buy CLGlo™ assay kits    

 

Buy CLGlo™-HT assay kits Available as assay kits and
contract research services.

 

CLGlo™ Assay Applications

• Viability, cell functionality, proliferation / cytotoxicity and cell number assays for virtually any cell line.
• Determine growth kinetics.
• Determine proliferation ability and/potential of engineered cell lines.
• Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other reagents on cell lines.
• High throughput in vitro toxicity testing and screening.

 

Special CLGlo™-HT Applications

• High throughput in vitro toxicity screening and testing.
• Can be incorporated into drug development ADME/Tox.
• Drug/agent toxicity ranking and comparison with primary stem cells, stem cell lines and ex vivo primary explanted cells.

 

Advantages of using CLGlo™

• The most sensitive of any assay readouts incorporating proven Bioluminomics™ Technology.
• Instrument-based, quantitative and fully standardized readout procedure.
• Available for adherent or non-adherent cell lines.
• Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
• High throughput capability.
• Reliably compare results over time.
• Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
• Easy to learn, fast to use.

 

Special Advantages of using CLGlo™-HT

In addition to the advantages of using STEMGlo™, the high throughput in vitro toxicity platform has the following special advantages:

• 96- and 384-well plate availability.
• Rapid turnaround.
• Complete assay calibration, standardization and validation according to regulatory requirements.

 

Species

• Multiple

 

Cell Lines Sources

Since cells lines are usually derived from spontaneously or genetically modified normal cells that continuously proliferate, CLGlo™ can be used for virtually any mammalian cell line source. CLGlo™ might also be used for Cell lines derived from non-mammalian species. 

 

Availability

CLGlo™ assays are available for:

• Research with 96-well plates
• In vitro toxicity screening and testing with 96- or 384-well plates

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents

• CLGlo™ Base Medium
• ATP standard
• ATP controls
• ATP Enumeration Reagent
• Sterile, 96- or 384-well plates, depending on assay use
• Non-sterile, 96- or 384-well plates, depending on assay use
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• STEMGlo™-HT
• XVPrime-Glo™

CLFluor™

A Fluorescence Assay Platform for Cell Lines and Tumor Cells

 

   Buy CLFluor™ assay kits    

All assays are available as kits
and contract research services.

 

CLFluor™ Assay Applications

• Viability, cell functionality, proliferation / cytotoxicity and cell number assays for virtually any cell line.
• Determine growth kinetics.
• Determine proliferation ability and/potential of engineered cell lines.
• Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other reagents on cell lines.

 

Advantages of using CLFluor™

• Uses a fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.
• Non-subjective, instrument-based and quantitative readout procedure.
  Available for adherent or non-adherent cells.
• Multiplexes with flow cytometry allowing cells to be detected using other fluorescent labels/markers.
• Multiplexes with luminescence assays.
• Multiplexes with gene expression assays etc for more information from a single sample.
• Flexibility to use virtually any in vitro cell protocol, reagents and growth factors/cytokines.
• High throughput capability.
• Easy to learn, fast to use.

 

Species

• Multiple

 

Cell Lines Sources

Since cells lines are usually derived from spontaneously or genetically modified normal cells that continuously proliferate, CLFluor™ can be used for virtually any mammalian cell line source. CLFluor™ might also be used for Cell lines derived from non-mammalian species. 

 

CLFluor™-96 Principle

• Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
• After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time: 2 hours.
• Uses a plate fluorometer or multiparameter plate reader to measure fluorescence with an excitation filter of 380-400nm and an emission filter of 505nm.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Fluorescence plate reader or multiparameter plate reader.

 

Kit Contents

• CLFluor™ Base Medium
• GF-AFC Reagent
• Sterile, 96-well plates, depending on assay use
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• STEMFluor™
• XVPrime-Fluor™

CLLight™

An Absorbance Assay Platform for Cell Lines and Tumor Cells

 

   Buy CLLight™ assay kits    

All assays are available as kits
and contract research services.

 

CLLight™ Assay Applications

• Viability, cell functionality, proliferation / cytotoxicity and cell number assays for virtually any cell line.
• Determine growth kinetics.
• Determine proliferation ability and/potential of engineered cell lines.
• Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other reagents on cell lines.

 

Advantages of using CLLight™

• Uses a absorbance plate reader or multiparameter plate reader, available in most laboratories.
• Non-subjective, instrument-based and quantitative readout procedure.
  Available for adherent or non-adherent stem cells.
• Multiplexes with gene expression assays etc for more information from a single sample.
• Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
• High throughput capability.
• Easy to learn, fast to use.

 

Species

• Multiple

 

Cell Line Sources

Since cells lines are usually derived from spontaneously or genetically modified normal cells that continuously proliferate, CLLight™ can be used for virtually any mammalian cell line source. CLLight™ might also be used for Cell lines derived from non-mammalian species. 

 

CLLight™-96 Principle

• Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction, colorimetric reaction.
• After culture, just add 20μl of the MTS reagent, mix and read absorbance at 490nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
• No solubilization step as in a MTT reaction. Replaces MTT assays.
• Read results between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Absorbance plate reader or multiparameter plate reader with a 490nm filter.

 

Kit Contents

• CLLight™ Base Medium
• MTS Reagent
• Sterile, 96-well plates
• Non-sterile, 96-well plate for background determination
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• STEMLight™ HT
• XVPrime-Light™