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Products - ColonyGro™ / CAMEO™  
 
  • ColonyGro™ CFU/CFC Assays

    for Research and Cell Therapy

  • CAMEO™-4 CFU/CFC Assays

    for Research & Cell Therapy

  • CAMEO™-96

    Standardized CFU Assays

Methylcellulose colony-forming unit CFU assay

ColonyGro™

Hematopoietic Stem and Progenitor Cell Assays. Quality controlled, methylcellulose, colony-forming cell reagents that allow a higher number of samples to be tested, same or lower price and greater performance than MethoCult™.

 

Buy ColonyGro™ Reagents

 

 

 

Application Note: 50 Years of Colony-Forming Assays. Comparison and Future

Comparison of Availability of CFC Assays from Different Suppliers

 

Uses of ColonyGro™ for Research
  • All applications involving lympho-hematopoietic cells with a manual or image analysis readout.
  • Detect clonal ability of cells.
  • Evaluate the differentiation status and/or potential of different lympho-hematopoietic cell populations.
  • Correlate in vivo with in vitro differentiation.
  • Differentiation effects of growth factors and or cytokines.
  • Evaluate ex vivo differential expansion.
  • Evaluate the effects of drugs, xeonbiotics or other agents on the differentiation/maturation process.  

 

Benefits to using ColonyGro™ Reagents
  • Same reagents used in CAMEO™-4 and CAMEO™-96 Assay Kits.
  • Same growth factor/cytokine cocktails used in CAMEO™-4, CAMEO™-96, HALO®, HemoFLUOR™ and HemoLIGHT™ Assay Kits.
  • Available to detect 8 different stem cell populations, and and 10 progenitor and precursor cell populations.
  • Includes equivalent competitor formulations.
  • Colonies can be counted after just 9-10 days.
  • Unlike competitor formulations, ColonyGro™ CFC-GEMM formulations actually contain TPO, allowing the production of erythroid, granulocyte, macrophage and megakaryocytes to be produced as defined for the CFC-GEMM populations.
  • Setup 44 samples/100mL with ColonyGro™, instead of only 33 with reagents from other suppliers.
  • Same or lower price than MethoCult™ CFU reagents.
  • Superior performance compared to MethoCult™ and similar reagents. 

 

 Species

  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus)
  • Dog
  • Rat
  • Mouse

 Cell Populations Detected using ColonyGro™ Reagents

The following cell populations are available in ColonyGro™. Those populations that have multiple growth factors will produce different colony types in the same culture. Please note that the number of colonies counted in culture containing multiple growth factors will be different to those with single growth factors, which only detect cells in a specific cell compartment. To see the stage of developemnt for each cell population please click here.

 

Cell Type Cell Population Detected (1) MethoCult™ Equivalent Growth Factor Cocktail Number of Samples(2)
Any None NE None 44
Stem cell HPP-SP 1, primitive stem cells NE 3,6,SCF,Flt3-L 44
Stem cell HPP-SP 2, and all lineages NE EPO,GM,2,3,6,7,SCF,TPO, Flt3-L 44
Stem cell CFC-GEMM 1, and all hematopoietic lineages NE EPO,GM,3,6,SCF,TPO, Flt3-L 44
Stem cell CFC-GEMM 2, and all hematopoietic lineages NE EPO,GM,3,6,SCF,TPO 44
Stem cell CFC-GEMM 3, and all hematopoietic lineages

H4435 "Enriched"

MethoCult Express

EPO,GM,G,3,6,SCF TPO(3) 44
Stem cell CFC-GEM 1, and E and GM lineages only(4) NE EPO,GM3,6,SCF 44
Stem cell CFC-GEM 2, and E and GM lineages only H4034 "Optimum" EPO,GM,G,3,SCF 44
Stem cell CFC-GEM 3, and E and GM lineages only H4434 "Classic" EPO,GM,3,SCF 44
Progenitor BFU-E 1, primitive E cells NE EPO,3,SCF 44
Progenitor BFU-E 2, E cells only H4430 EPO (high dose) 44
Precursor CFU-E, mature E cells NE EPO (low dose) 44
Progenitor GM-CFC 1, primitive GM cells H4534 "Classic" GM,3,SCF 44
Progenitor GM-CFC 2, primitive GM and neutrophils H4035 "Optimum" GM,G,3,SCF 44
Progenitor GM-CFC 3, GM cells only NE GM alone 44
Precursor G-CFC, neutrophils only NE G alone 44
Precursor M-CFC, macrophages only NE M alone 44
Progenitor Mk-CFC 1, primitive megakryocytes NE TPO,3,SCF 44
Progenitor Mk-CFC 2, megakaryocytes only NE TPO alone 44
Progenitor B-CFC, B-lymphocytes NE 7 alone 44

NE = No equivalent product available.

(1) = Accepted nomenclature of cell populations detected by the colony-forming cell assay.

(2) = Number of samples that can be performed from 100mL by setting up a total volume of 2.5mL, including 10% cell volume), sufficient for duplicate 1mL cultures in 35mm Petri dishes.

(3) = Contains TPO to stimulate megakaryopoiesis. The equivalent MethoCult™ product does not contain TPO and therefore cannot be designated as a CFC-GEMM stem cell population, but rather a CFC-GEM population.

(4) = E for erythropoietic; GM for granulocyte-macrophage.

 ColonyGro™ and MethoCult™: A Comparison

 

How ColonyGro for CFC-GEMM compares to MethoCult H4435  

Click image for larger view

The graph to the left shows a cell dose response comparison between ColonyGro™ for the primitive hematopoietic stem cell population, CFC-GEMM 1, and the nearest MethoCult™ equivalent population detected with H4435 media. The difference between the the growth factors is given in the table above, but whereas the CFC-GEMM 1 growth factor cocktail contains Flt3-Ligand and thrombopoietin (TPO), to induce the production of megakaryocytes, MethoCult™ H4435, contains neither of these components. Consequently, MethoCult™ H4435 cannot produce megakaryocytes and therefore does not stimulate the production of primitive stem cells and cannot be designated as a CFC-GEMM population; MethoCult™ H4435 actually stimulates a more mature stem cell population.

The HemoGenix® direct equivalent of MethoCult™ H4435 is the CFC-GEMM 3 stem cell population. However, unlike H4435, ColonyGro™ for CFC-GEM 

 ColonyGro™ Reagents, Use and Equipment Required

  • 100mL of ColonyGro™ Reagent
  • Sufficient for 44 duplicate samples
  • 2.5ml total volume prepared/sample
  • 0.25ml sample cell suspension volume required
  • 1ml plated in 2 x 35mm Petri dishes

 

Use

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Inverted microscope with a magnification of 40x - 100x.

 ColonyGro™ Information Flyer, Quick Guide and Assay Manual

CAMEO-4 Methylcellulose Colony-Forming Unit  CFU Assays

CAMEO™-4 

Hematopoietic Stem and Progenitor Cell Assays. Complete, miniaturized, methylcellulose colony-forming cell assay that are completed in just 10 days with greater flexibility, larger number of samples and superior quality and performance compared to MethoCult™

 

Buy CAMEO™-4 Assay Kits

 

CAMEO™-4 Contract Services

 

 

Application Note: 50 Years of Colony-Forming Assays. Comparison and Future

Comparison of Availability of CFC Assays from Different Suppliers

 
Uses/Applications of CAMEO™-4 
  • All areas of hematopoietic research and cellular therapy requiring a methylcellulose, clonal assay.
  • Evaluate the differentiation status and/or potential of different lympho-hematopoietic cell populations.
  • Correlate in vivo with in vitro differentiation.
  • Differentiation effects of growth factors and or cytokines.
  • Evaluate ex vivo differential expansion.
  • Evaluate the effects of drugs, xeonbiotics or other agents on the differentiation/maturation process. (Please note that CAMEO™-4 is not recommended for toxicity testing.)

Benefits of using CAMEO™-4
  • Miniaturized assay format requires 1/10 of the volume of reagents, target cells and other sample requirements than the traditional 35mm Petri dish CFC assay.
  • Greater number of replicates (4) improves statistics.
  • Smaller growth surface for more accurate and faster colony counting.
  • No meniscus; allows evaluation of colonies to the well edge.
  • Colonies counted after just 9-10 days, compared to 14 days for MethoCult® reagents.
  • CAMEO™-4 assay kits contain sufficient reagents and culture plates for 50 samples.
  • Uses the same growth factor cocktails as ColonyGro™, CAMEO™-96, HALO®-96 Research and HALO®-96 PCAEQ , allowing a direct comparison and correlation between all these assays.
  • Complete flexibility; evaluate new growth factors and/or cytokines or choose from 17 different cell populations and 8 different species (see below).
  • Quality controlled media and superior performance compared to MethoCult™

 Species
  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
  • Horse
  • Pig
  • Sheep
  • Dog
  • Rat
  • Mouse

 Cell Populations detected using CAMEO-4™

The following cell populations are available in ColonyGro™. Those populations that have multiple growth factors will produce different colony types in the same culture. Please note that the number of colonies counted in culture containing multiple growth factors will be different to those with single growth factors, which only detect cells in a specific cell compartment. To see the stage of developemnt for each cell population please click here.

 

Cell Type Cell Population Detected (1) MethoCult™ Equivalent Growth Factor Cocktail Number of Samples(2)
Any None NE None 50
Stem cell HPP-SP 1 NE 3,6,SCF,Flt3-L 50
Stem cell HPP-SP 2 NE EPO,GM,2,3,6,7,SCF,TPO, Flt3-L 50
Stem cell CFC-GEMM 1 NE EPO,GM,3,6,SCF,TPO, Flt3-L 50
Stem cell CFC-GEMM 2 NE EPO,GM,3,6,SCF,TPO 50
Stem cell CFC-GEMM 3

H4435 "Enriched"

MethoCult Express

EPO,GM,G,3,6,SCF TPO(3) 50
Stem cell CFC-GEM 1 NE EPO,GM3,6,SCF 50
Stem cell CFC-GEM 2 H4034 "Optimum" EPO,GM,G,3,SCF 50
Stem cell CFC-GEM 3 H4434 "Classic" EPO,GM,3,SCF 50
Progenitor BFU-E 1 NE EPO,3,SCF 50
Progenitor BFU-E 2 H4430 EPO (high dose) 50
Precursor CFU-E NE EPO (low dose) 50
Progenitor GM-CFC 1 H4534 "Classic" GM,3,SCF 50
Progenitor GM-CFC 2 H4035 "Optimum" GM,G,3,SCF 50
Progenitor GM-CFC 3 NE GM alone 50
Precursor G-CFC NE G alone 50
Precursor M-CFC NE M alone 50
Progenitor Mk-CFC 1 NE TPO,3,SCF 50
Progenitor Mk-CFC 2 NE TPO alone 50
Progenitor B-CFC NE 7 alone 50

NE = No equivalent product available.

(1) = Accepted nomenclature of cell populations detected by the colony-forming cell assay.

(2) = Number of samples that can be performed from 38mL by setting up a total volume of 0.6mL, including 10% cell volume), sufficient for quadruplicate 0.1mL cultures in 4-well, 35mm Petri dishes.

(3) = Contains TPO to stimulate megakaryopoiesis. The equivalent MethoCult™ product does not contain TPO and therefore cannot be designated as a CFC-GEMM stem cell population, but rather a CFC-GEM population.

 

 

How to count colonies in the CFU assay using the CAMEO-4 assay kit 

Click image for larger view

Both ColonyGro™ and CAMEO™-4 incorporate the same growth factor cocktails. 

  1. Whereas ColonyGro™ uses 35mm Petri dishes, CAMEO™-4 is miniaturized using 4-wells in a 35mm Petri dish that only require 0.1mL/well of reagent rather then 1mL of reagent. In short, the growth surface area is smaller for CAMEO™-4 than it is for ColonyGro™. As a result, the increased cell interaction allows the cells to grow faster producing colonies in a shorter period of time.
  2. The graph to the left shows that there is a direct correlation between ColonyGro™ and CAMEO™-4. This means that the assays are interchangable; one can replace the other. Since both ColonyGro™ and CAMEO™-4 are the same price, it is obviously more economical to use CAMEO™-4 than ColonyGro™, because with CAMEO™-4, the culture plates are included in the assay kit.

 Relationship between CAMEO™-4 (or ColonyGro™) and HALO®

Both CAMEO™-96 and HALO® were derived from the traditional colony-forming cell/unit (CFC/CFU) assay represented by CAMEO™-4 and ColonyGro™. It would therefore be expected that a direct relationship or correlation exist between these different assays. To see how these assays relate to each other and why HALO® can replace any of the CFC/CFU assays, please clcik here.

 Use and Major Equipment Required
Use

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Inverted microscope with a magnification of 40x - 100x.

 CAMEO™-4 Assay Kit Contents
Kit Contents
  • CAMEO™-4 Reagent. Choose from the cell populations and species abov
  • 50 x 4-well cell culture plates

 CAMEO™-4 Information Flyer, QuickGuide and Assay Manual

CAMEO-96 methylcellulose colony-forming unit CFU assay for proliferation and differentiation using ATP bioluminescence

CAMEO™-96

Hematopoietic Stem and Progenitor Cell Assays. Complete, standardized, 96-well, methylcellulose, colony-forming cell, 7-10 day proliferation and differentiation assay.

 

Buy CAMEO™-96 Assay Kits

 

CAMEO™-96 Contract Services

 

Application Note: 50 Years of Colony-Forming Assays. Comparison and Future

Comparison of Availability of CFC Assays from Different Suppliers

Application / Research Areas
  • Hematopoiesis
  • Cell proliferation
  • Cell differentiation
  • Stem cell research
  • Cell viability
  • Clonal cell growth

 

Uses of CAMEO™-96
  • Detection of proliferation AND differentiation of lympho-hematopoietic cells under clonal conditions in a single assay.
  • Standardization of the colony-forming cell (CFC) assay.
  • Compare and evaluate proliferation and differentiation status and/or potential of different lympho-hematopoietic cell populations from up to 8 species.
  • Correlate in vivo with in vitro proliferation and differentiation.
  • Distinguish between growth factors and cytokines that have a proliferation effect and/or a differentiation effect.
  • Evaluate ex vivo expansion.

 

Benefits of Using CAMEO™-96
  • CAMEO™-96 is the only assay in which both proliferation and differentiation can be determined simultaneously, using different readouts, in the same assay.
  • The only assay that allows standardization of the CFU/CFC assay using standards and controls.
  • The only assay that allows conversion of colony counts into standardized and quantitative ATP concentrations.
  • Complete flexibility; evaluate growth factors and/or cytokines or choose from 19 different cell populations and 8 species.
  • Incorporate powerful and proven ATP bioluminescence readout technology to measure cell proliferation.

 

 Species
  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
  • Horse
  • Pig
  • Sheep
  • Dog
  • Rat
  • Mouse

 Cell Populations Detected using CAMEO™-96

The following cell populations are available as CAMEO™-96 Complete Assay Kits. Those populations that have multiple growth factors will produce different colony types in the same culture. Please note that the number of colonies counted and their proliferation in cultures containing multiple growth factors will be different to those with single growth factors, which only stimulate cells in a specific cell compartment. To see the stage of developemnt for each cell population please click here.

 

Cell Type Cell Population Detected (1) MethoCult™ Equivalent Growth Factor Cocktail Suggested Number of Samples(2)
Any None NE None 24, 16 or 12
Stem cell HPP-SP 1 NE 3,6,SCF,Flt3-L 24, 16 or 12
Stem cell HPP-SP 2 NE EPO,GM,2,3,6,7,SCF,TPO, Flt3-L 24, 16 or 12
Stem cell CFC-GEMM 1 NE EPO,GM,3,6,SCF,TPO, Flt3-L 24, 16 or 12
Stem cell CFC-GEMM 2 NE EPO,GM,3,6,SCF,TPO 24, 16 or 12
Stem cell CFC-GEM 1 NE EPO,GM3,6,SCF 24, 16 or 12
Stem cell CFC-GEM 2 H4034 EPO,GM,G,3,SCF 24, 16 or 12
Progenitor BFU-E 1 NE EPO,3,SCF 24, 16 or 12
Progenitor GM-CFC 1 H4534 GM,3,SCF 24, 16 or 12
Progenitor Mk-CFC 1 NE TPO,3,SCF 24, 16 or 12
Progenitor T-CFC NE 2 alone 24, 16 or 12
Progenitor B-CFC NE 7 alone 24, 16 or 12

NE = No equivalent product available.

(1) = Accepted nomenclature of cell populations detected by the colony-forming cell assay.

(2) = The number of samples is based on the number of replicate wells/sample. If 4 replicate wells/sample are used, 24 samples/plate can be detected. Using 6 replicate wells/sample, 16 samples can be detected and for 8 replicates wells/samples prepared in columns, 12 samples can be measured.

 Relationship between CAMEO™-4 (or ColonyGro™), CAMEO™-96 and HALO®

Both CAMEO™-96 and HALO® were derived from the traditional colony-forming cell/unit (CFC/CFU) assay represented by CAMEO™-4 and ColonyGro™. It would therefore be expected that a direct relationship or correlation exist between these different assays. To see how these assays relate to each other and why HALO® can replace any of the CFC/CFU assays, please click here.

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Inverted microscope with a magnification of 40x - 100x.
Plate luminometer or multiparameter plate reader with "glow" luminescence capability.

 CAMEO™-96 Assay Kit Contents
  • CAMEO™-96 Reagent
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile-96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

 CAMEO™-96 Information Flyer, QuickGuide and Assay Manual