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Products - CellExpand™ / HALO®     Page   1  |  2  |  3  
 
  • CellExpand™ and

    CellExpand-SF Reagents

  • HALO®-96 Research:

    Cell Proliferation Assays 

  • HALO®-96 PREP:

    Self-Renewal

  • HALO®-96 PCAEQ:

    CFU-Equivalent

  • HALO-
    Real Time

CellExpand - In vitro expansion media for hematopoietic stem and progenitor cells

CellExpand™

Hematopoietic Stem and Progenitor Cell Expansion Reagents complete with growth factor cocktails in HemoGro™ Medium to stimulate up to 14 different populations from up to 5 different species

 

Buy CellExpand™ Reagents

 

 

Now available in Serum-Free (for Human, Primate and Mouse Cells) and Low Serum (all species) formulations as 15mL, 40mL or in bulk quantities

 

Application / Research Areas
  • Hematopoiesis
  • Cell proliferation
  • Cell differentiation
  • Cell expansion

 

Uses of CellExpand™
  • Expansion of stem and progenitor lympho-hematopoietic cells in suspension cultures.
  • Used for 96-well, 24-well, 12-well or 6-well plate suspension cultures or in large culture flask volumes.
  • Allows easy and rapid high-content screening for both proliferation and differentiation processes.
  • High throughput flow cytometry.
  • Cell signaling studies.
  • DNA, RNA analysis and other molecular biology methods.
  • Mechanism of Action (MOA) studies.
  • Evaluate the effects of drugs, xeonbiotics or other agents on the differentiation/maturation process.
  • Gene transfection and targeting studies, e.g. CRISPR-Cas9.

 

Benefits of using CellExpand™ Reagents
  • Available low serum or serum-free (human and primate only) formulations.
  • Used in all HALO®, HemoFLUOR™ and HemoLIGHT™ Assay Kits.
  • Available for 6 stem cell populations and 7 progenitor cell populations.
  • Setup as many replicates as necessary.
  • Allows easy, rapid and accurate dispensing using normal pipettes or liquid handlers.
  • CellExpansion™ Master Mixes allow for cell interactions thereby providing: (1) Shorter lag time to initiate proliferation; (2) More rapid culture completion; (3) Greater sensitivity.

 Species

CellExpand™ Low Serum Reagents are available for the for the following species:

  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus)
  • Dog
  • Rat
  • Mouse

CellExpand Serum-Free is now available for human, primate and mouse cells.

 CellExpand™ for Lympho-Hematopoietic Stem Cells and Hematopoietic Progenitor Cells

CellExpand™ Reagents are available for the following cell populations:

Stem Cells (SC)

  • SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L
  • SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
  • SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
  • SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
  • SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
  • SC-GEM 2: EPO, GM-CSF, IL-3, SCF 


Progenitor Cells (P)

  • P-BFU 1: EPO, IL-3, SCF
  • P-BFU 2: EPO (high dose)
  • P-GM 1: GM-CSF, IL-3, SCF 
  • P-GM 3: GM-CSF
  • P-Mk 1: TPO, IL-3, SCF
  • P-Bcell: IL-7
  • (*) denotes that these CellExpand™ products are only available for human cells and as a serum-free formulation.

Use HemoGro™-LS or HemoGro™-HPSF for controls (no growth factors) 

 CellExpand™ for Human T-Cell Culture and Expansion

With the development and use of CAR-T cells and other T-cell populations for cellular therapy, HemoGenix® has expanded its offering of CellExpand™ reagents specifically for T-cells.

Ready-to-use, single strength CellExpand™ Human T-Cell Culture and Expansion Reagents (40mL bottles):

  • P-Tcell: IL-2 (requires addition of co-stimulators)
  • P-Tcell: Co-stimulators CD3 + CD28*
  • P-Tcell: IL-2 + co-stimulators CD3 + CD28*
  •  

    Concentrated (10x) CellExpand™ Human T-Cell Culutre and Expansion Reagents that are added to ImmunoGro™ Serum-Free Base Medium:
  • P-Tcell: IL-2 (requires addition of co-stimulators)
  • P-Tcell: Co-stimulators CD3 + CD28*
  • P-Tcell: IL-2 + Co-stimulators CD3 + CD28*
  • ImmunoGro™ Base Medium, serum-free.
  •  

    To measure T-cell proliferation and expansion use either:
  • ImmunoGlo™-96, ImmunoFluor™-96 or ImmunoLight™-96 with no growth factors or co-stimulators
  • OR
  • ImmunoGlo-96 TCP, ImmunoFluor™-96 TCP or ImmunoLight™-96 TCP with growth factors and/or co-stimulators.

 Use

For Research Use Only. Not for clinical diagnostic use

HALO-96 Research. The "Gold Standard" for analyzing hematopoietic stem and progenitor cell proliferation ability and potential

HALO®-96 Research

Cell Proliferation Assays for Hematopoietic Stem and Progenitor Cells. Advanced, 96-well, 5-7 Day, bioluminescence proliferation assay kits to measure up to 16 different cell populations from 8 species

 

Buy HALO®-96 Research assay kits

 

HALO® Contract Services

 

Application Note: 50 Years of Colony-Forming Assays. Comparison and Future

Comparison of Availability of CFC Assays from Different Suppliers

Application / Research Areas
  • Hematopoiesis
  • Lymphopoiesis
  • Stem cell biology
  • Cell proliferation
  • Cell viability 

 

Uses of HALO®-96 Research
  • For all human and animal lympho-hematopoietic research applications.
  • Detect and measure proliferation potential and ability of human and animal stem and progenitor cells, including T-cell progenitor cells.
  • Reliably and reproducibly replaces all applications of the colony-forming assay (CFU/CFC).
  • Measure and compare proliferation status and/or potential between tissues and cell populations.
  • Experimental transplantation models.
  • Effects of growth factors and/or cytokines.
  • In vivo to in vitro assays.
  • Gene targeting assays, e.g. CRISPR-Cas9.

 

Benefits of using HALO®-96 Research 
  • HALO®-96 Research measures the viability, proliferation ability and potential of lympho-hematopoietic human and animal cells. (The colony-forming unit (CFU) or cell (CFC) assay, does not measure proliferation; it is a differentiation assay because colonies are identified by the differentiation and maturation of the cells that form the colonies. Please see CAMEO™-4 and CAMEO™-96). See CFC and HALO®: The Facts.
  • Hematopoietic stem and progenitor cell proliferation is measured using the cell's ability to produce intracellular ATP as a limiting substrate for a luciferin-luciferase reaction.
  • HALO®-96 Research is an ATP bioluminescence assay producing light that is measured in a luminescence plate reader.
  • All results are non-subjective, instrument-based and quantitative.
  • HALO®-96 Research incorporates is fully standardized using external standards and control included with the assay kit.
  • HALO®-96 Research allows the direct comparison of results between samples and over time without the normalization of data.
  • HALO®-96 Research incorporates proprietary Suspsension Expansion Culture™ (SEC™) Growth Medium Technology to distinguish up to 7 different stem cell and 9 different progenitor cell populations from 8 differnt species. SEC™ Growth Media is available in low serum formulation for 8 species and serum-free formulation for 3 species.
  • Also available as 4-, 5- and 7-Population "Global" assays  using low serum or serum-free SEC™ Growth Media.
  • HALO®-96 Research provides results in just 4 days for animal cells and 5 days for human cells.
  • HALO®-96 Research can be learnt in just 1 day (no colony counting required) and is fast to use saving time and money.
  • Combine (multiplex) HALO®-96 Research with other assay readouts such as flow cytometric phenotypic analysis or gene expression assays.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species

HALO®-96 Research is available with a low serum formulation of SEC™ Growth Media for the following species:

  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
  • Horse
  • Pig
  • Sheep
  • Dog
  • Rat
  • Mouse

 

HALO®-96 Research is also available with serum-free SEC™ Growth Media for:

  • Human
  • Primate
  • Mouse

 Individual and "Global" Cell Populations Detected using HALO®-96 Research
See Lympho-Hematopoietic System to view cell populations and growth factor/cytokine cocktails).

HALO®-96 Research is available for the following cell populations:

Stem Cells (download the Stem Cell Population Organization Chart)

  • SC-HPP X: IL-3, IL-6, SCF, Flt3-L (only for human cells and only serum-free)
  • SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L
  • SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
  • SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
  • SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
  • SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
  • SC-GEM 2: EPO, GM-CSF, IL-3, SCF
  • SC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

 

Primitive Stem Cell-Producing, T-Lymphocyte Cell Populations

  • SC-HPP XT: IL-3, IL-6, SCF, Flt3-L + CD3 + CD28 (only for human cells and only serum-free; see also P-Tcells)
  • SC-HPP 1T: IL-3, IL-6, SCF, Flt3-L + IL-2, CD3 + CD28 (only for human cells and only serum-free; see also P-Tcells)

 

Progenitor Cells (P)

  • P-BFU 1: EPO, IL-3, SCF
  • P-BFU 2: EPO alone
  • P-GM 1: GM-CSF, IL-3, SCF
  • P-GM 2: GM-CSF, G-CSF, IL-3, SCF
  • P-GM 3: GM-CSF alone
  • P-Mk 1: TPO, IL-3, SCF
  • P-Mk 2: TPO alone

 

Lymphopoietic cells

  • P-Tcell: IL-2 alone
  • P-Bcell: IL-7 alone

No growth factors: Add growth factors/cytokines of choice.

 

HALO®-96 Research "Global" Population Assays

4-Population "Global" Assays include reagents to measure:

  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • Background controls

 

5-Population "Global" Assays include reagents to measure:

  • SC-HPP 2
  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • Background control

 

7-Population "Global" Assays include reagents to measure:

  • SC-HPP 2
  • SC-GEMM 1
  • P-BFU 1
  • P-GM 1
  • P-Mk 1
  • P-Tcell
  • P-Bcell
  • Background control

 Examples using HALO®-96 Research

Stem cell proliferation potential HALO-96 Research assay kits

Click image for larger view

Measuring Proliferation Potential

Proliferation potential is measured by performed a cell dose response and calculating the slope of the linear regression. The steeper the slope, the greater the proliferation potential of the cell population. The greater the proliferation potential, the more primitive the cell population. HALO®-96 Research and other HemoGenix® instrument-based assays can all be used to measure proliferation potenti

 

Example of a 7-population comparison of lympho-hematopoietic cell proliferation between human tissues using HALO-96 Research

Click image for larger view

Example of a 7-Population Comparison Between Human Tissues

The graph shows the response of 7 lympho-hematopoietic cell populations derived from mobilized peripheral blood CD34+ cells, bone marrow and umbilical cord blood mononuclear cells. Please note that whereas mobilized peripheral blood CD34+ cells are shown at 500 CD34+/well, bone marrow and umbilical cord blood are shown at 5,000 mononuclear cells/well.

 

Example of a 7-population comparison of lympho-hematopoietic cell proliferation between different species using HALO-96 Research

Click image for larger view

Comparison of 7-Population Response between Species

The graph shows the response of 7 lympho-hematopoietic populations, SCHPP 2, SC-GEMM 1, P-BFU 1, P-GM 1, P-Mk 1, P-Tcell, P-Bcell and background, from sheep, pig, dog, rat and mouse bone marrow. 

 

T-lymphocyte cells produced from primitive lympho-hematopoietic stem cells using HALO-96 Research

Click image for larger view

Production of T-Lymphocytes from Primitive Lympho-Hematopoietic Stem Cells

Human bone marrow High Proliferative Potential-Stem and Progenitor Cells (SC-HPP) were stimulated with various factors and lymphocyte co-factors to induce the cells out of quiecence and into the T-cell lineage. Such studies can be combined with low cytometric phenotypic analysis to determine different T-cell populations. 

 

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use

 

Major Equipment Required

Plate luminometer or multimode plate reader with "glow" luminescence measuring capability.

 Kit Contents
  • HALO®-96 Research Master Mix(s). Please choose between low serum or serum-free formulations.
  • ATP standard
  • ATP high and low controls (not included with research kits)
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

 HALO®-96 Research Information Flyer, QuickGuide and Assay Manual

HALO-96 PREP. Detection of stem cell self-renewal and expansion potential

HALO®-96 PREP

Cell proliferation, self-renewal and expansion potential assay for primitive lympho-hematopoietic stem cells.

 

Buy HALO®-96 PREP assay kits

 

HALO®-96 PREP Contract Services

 

 

Application / Research Areas
  • Hematopoiesis
  • Stem cell self renewal
  • Stem cell expansion
  • Cell proliferation
  • Cell viability 

 

Uses of HALO®-96 PREP
  • Designed specifically for the primitive lympho-hematopoietic cell population, HPP-SP (High Proliferative Potential - Stem and Progenitor Cell).
  • Uses secondary re-plating technology to substantiate the presence of primitive stem cells.
  • Faster and more easier LTC-IC assay alternative.
  • Experimental transplantation models.
  • In vivo to in vitro assays
  • Gene targeting assays, e.g. CRISPR-Cas9.

 

Benefits of using HALO®-96 PREP for Your Research
  • The only commercially available assay that can detect and measure proliferation, self-renewal and expansion potential of primitive lympho-hematopoietic stem cells (HPP-SP).
  • Distinguish between quiescent and proliferating primitive stem cells.
  • Used to "prime" primitive stem cells out of quiescence and into cell cycle.
  • A faster, easier and lower-priced alternative to LTC-IC assays.
  • HALO® utilizes proprietary Suspension Expansion Culture™ (SEC™) growth medium and ATP bioluminescence technology for the most accurate, sensitive and reliable assays available.
  • Reliably compare results from one experiment to another over time by calibrating and standardizing the assay.
  • Multiplex HALO®-96 PREP with flow cytometry or molecular expression analysis for even more information from the same sample.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species

HALO®-96 PREP is available for the following species:

  • Human
  • Non-human primate
  • Rat
  • Mouse

 Cell Populations Detected
  • SC-HPP 1: Quiescent, primitive, lympho-hematopoietic stem cell population.
  • SC-HPP 2: "Fully stimulated" primitive lympho-hematopoietic stem cell population.

 Use and Major Equipment Required

For Research Use Only.

 

Major Equipment Required

Plate luminometer or multimode plate reader with "glow" luminescence measuring capability. 

 Kit Contents
 
  • HALO®-96 PREP Master Mixes
  • ATP standard
  • ATP enumeration reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

HALO-96 PCAeq. An advanced ATP bioluminescence progenitor cell equivalent replacement assay for the CFU assay

HALO®-96 PCAEQ

Cell Proliferation Assays for Hematopoietic Stem and Progenitor Cells. Complete, methylcellulose-free, but MethoCult™-equivalent, 96-well, 5-day assays kits for cord blood banks and cell processing laboratories

 

Buy HALO®-96 PCAEQ assay kits

 

 

 

Application Note: 50 Years of Colony-Forming Assays. Comparison and Future

Comparison of Availability of CFC Assays from Different Suppliers

Applications / Research Area
  • Hematopoiesis
  • Cell therapy
  • Cell proliferation
  • Cell viability 

 

Uses of HALO®-96 PCAEQ
  • A standardized, ATP bioluminescence, instrument-based, colony-forming unit (CFU) - equivalent assay for cell processing laboratories who want to supplement present "Minimal Testing Criteria" with "Best Practice Criteria Testing". HALO®-96 PCAEQ incorporates the same growth factor cocktails as MethoCult reagents (see below).
  • HALO®-96 PCAEQ is allowed by the FDA, AABB and NetCord-FACT and the NMDP has indicated that additional fields can be added to their cord blood database to upload results other than those of the CFU assay.

 

Benefits of using HALO®-96 PCAEQ in the Stem Cell Processing Laboratory
  • Transforms the "classic" and subjective CFC assay into an instrument-based, fully standardized and validated assay from which meaningful results regarding the proliferation ability of the target cells can be obtained.
  • Incorporates the same growth factor/cytokine cocktails used for MethoCult reagents for direct assay-to-assay comparison and correlation.
  • Faster, easier to use, less expensive and more accurate than any traditional CFU assay, even using automated colony counting.
  • HALO®-96 PCAEQ utilizes proprietary Suspension Expansion Culture™ (SEC™) growth media and ATP bioluminescence technology, the most advanced and cost-effective hematopoietic in vitro cellular functionality assay system available.
  • HALO®-96 PCAEQ is a "Best Practice Criteria" assay and is one of the cell therapy assays included in the HemoGenix® Proficiency Testing Program using HALO®-PT.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 HALO®-96 PCAEQ Assays that Replace MethoCult

 

HALO®-96 PCAEQ is a methylcellulose CFU-equivalent assay that can be used by cord blood banks and hematopoietic stem cell transplantation centers in place of any CFU assay

The table below shows the HALO®-96 PCAEQ assays that are equivalent to MethoCult reagents used in the stem cell processing laboratory. Please click on the Catalog Number to see more information and price.

(Please note that for cord blood banks applying for a BLA, the FDA requires validation of all assays. Unlike HALO®-96 PCAEQ, neither ColonyGro™, CAMEO™-4 nor MethoCult® reagents include standards and controls and can therefore not be validated according to FDA regulations).

HemoGenix® Assays that are Equivalent to MethoCult™ Reagents

HALO®-96 PCAEQ

Number

HALO®-96 PCAEQ

Non-Clonal, Non-Methylcellulose Cell Population

Equivalent Methylcellulose CFU Cell Population

Equivalent

MethoCult™

Reagent

Growth Factor Cocktail
PCA5

SC-GEMM 3

CFC-GEMM 3

H4435 "Enriched" EPO, GM-CSF, G-CSF, IL-3, IL-6, SCF + TPO(#)
PCA1

SC-GEM 2

CFC-GEM 2

H4434 "Classic" EPO, GM-CSF, IL-3, SCF
PCA2

SC-GEM 3

CFC-GEM 3

H4034 "Optimum" EPO, GM-CSF, G-CSF, IL-3, SCF
PCA3

P-GM 1

GM-CFC 1

H4534 "Classic" GM-CSF, IL-3, SCF
PCA4

P-GM 2

GM-CFC 2

H4035 "Optimum" GM-CSF, G-CSF, IL-3, SCF

(#) denotes that the HemoGenix® formulation of this product includes TPO that stimulates the production of megakaryopoiesis. MethoCult and other competitor formulations of this specific product do not include TPO, do not stimulate the production of megakaryocytes and threfore do not detect the CFC-GEMM stem cell population.

 Tissues
Tissues

HALO®-96 PCAEQ can be used for the following tissues:

  • Mobilized peripheral blood
  • Umbilical cord blood
  • Bone marrow
  • Purified cell populations for any of the above tissues.

 Cell Purity Requirements for Detection

The recomended cell purity is a mononuclear cell (MNC) fraction. A total nucleated cell (TNC) fraction is not recommended as this contains high concentrations of cell impurities, such as red blood cells, neutrophils, platelets and other cells that severely underestimate and even inhibit the detection of progenitor cells. 

 Replacing the CFU/CFC Assay with HALO®-96 PCAEQ

 

HALO-96 PCAeq: Improving the amount and quality of data using HALO-96 PCAeq over the CFU assay

Click image for larger view

Replacing the CFU/CFC Assay with HALO®-96 PCAEQ
The diagram to the left shows the results from HALO®-96 PCAEQ and CAMEO™-4, performed in parallel for human bone marrow and umbilical cord blood mononuclear cells (MNC). Although proliferation can be measured on days 5, 6 or 7, the results shown are from day 7 for HALO®-96 PCAEQ and colony counts from days 7 and 10 for CAMEO™-4. Results from day 7 for both assays can be directly compared with each other. Notice that equivalent results are obtained for both assays. Also notice that colony counts do not change significantly from day 7 to day 10. This is to be expected since once all of the colony-forming cells present in the culture start to proliferate, no new colonies will be formed. The existing colonies only grow in size due to proliferation of the cells. However, this information is lost to the user when working with a CFU/CFC assay. (Unless proper controls are included, automated colony counters and image analysis cannot provide quantitative data on cell proliferation in colonies).  In contrast, cell proliferation increases exponentially over the first 10 days of culture and can be quantified using HALO®-96 PCAEQ (See HALO®-96 SPC-QC to see how proliferation increases). The results clearly indicate that the "classic" CFU/CFC assay can be replaced by HALO®-96 PCAEQ or any other HALO® assay, all of which provide quantitative, standardized and reliable results that can be compared over time. The equivalent HALO®-96 PCAEQ assays for MethoCult® reagents are shown. 

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 Kit Contents
Kit Contents
  • HALO®-96 PCAEQ Master Mix
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-wll plates
  • Sterile, adhesive foil covers
  • Instruction manual.

 HALO®-96 PCAEQ Information Flyer, QuickGuide and Assay Manual

HALO-Real Time: Providing information of hematopoietic stem and progenitor cell growth that has never be possible before

HALO®-Real Time

Cell Proliferation and Viability Assays in Real Time for Lympho-Hematopoietic Stem/Progenitor Cells Using a Bioluminescence Endpoint

 

Buy HALO®-Real Time assay kits

 

 

 

 Applications / Research Areas
  • Hematopoiesis
  • Stem cell biology
  • Cell proliferation
  • Cell viability
  • Cell therapy

 

Uses of HALO®-Real Time
  • Follow lympho-hematopoietic stem and progenitor cells in real time, as they grow in culture.
  • Detect cell viability and proliferation ability at any time in vitro.
  • Establish kinetic growth curves.
  • Measure cell doubling time.
  • Measure cell cyle parameters at different time points.
  • Determine and measure the presence of different cell populations with time by flow cytometric, phenotypic analysis.
  • Determine if frozen cell samples will grow, prior to using units of cells (e.g. cord blood).
  • Determine how different culture conditions, cell interactions, stimulators affect cell proliferation.
  • Determine the onmset of cytotoxicity by drugs and xenobiotics.

 

Benefits of using HALO®-Real Time
  • HALO®-Real Time can be used as both a real time assay or as an end-point assay.
  • It can be combined with a standardized ATP bioluminescence assay such as HALO®-96 Research or any cellular therapy or toxicity assay.
  • Cultures can be setup in 96- or 384-well plates (included with the assay kits).
  • HALO®-Real Time is a non-destructive, non-lytic assay, allowing it to be combined (multiplexed) with virtually any other end-point assay.
  • HALO®-Real Time is available for up to 7 different stem cell and 9 different progenitor cell populations derived from 5 different species.
  • The HALO®-Real Time reagent can be added at the start of culture or at any time after culture to follow the cells for up to 3-5 days.
  • HALO®-Real Time incorporates HALO® Master Mix Growth Medium with growth factors and uses Suspension Expansion Culture™ (SEC™) Technology similar to that for all HALO®, HemoFLUOR™ and HemoLIGHT™ assays.
  • Fast to learn, easy to use. All you need are cells.

 Species

HALO®-Real Time is available for the following species:

  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
  • Dog
  • Rat
  • Mouse

 Cell Populations Detected using HALO®-Real Time
See Lympho-Hematopoietic System to view cell populations and growth factor/cytokine cocktails)

The following proliferation status or potential can be detected for individual populations using HALO®-96 SPCA:

Stem Cells (SC)

SC-HPP 1: IL-3, IL-6, SCF, TPO, Flt3-L

SC-HPP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L

SC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L

SC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO

SC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF

SC-GEM 2: EPO, GM-CSF, IL-3, SCF

SC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

Progenitor Cells (P)

P-BFU 1: EPO, IL-3, SCF

P-BFU 2: EPO

P-GM 1: GM-CSF, IL-3, SCF

P-GM 2: GM-CSF, G-CSF, IL-3, SCF

P-GM 3: GM-CSF

P-Mk 1: TPO, IL-3, SCF

P-Mk 2: TPO

Lymphopoietic cells

P-Tcell: IL-2 (requires addition of co-stimulator)

P-Bcell: IL-7

No growth factors: Add growth factors/cytokines of choice.

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use

Major Equipment Required

Plate luminometer or multimode plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
  • HALO®-96 Master Mix
  • HALO®-Real Time Reagent
  • Sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

 HALO®-Real Time Information Flyer, QuickGuide and Assay Manual

HALO®-Real Time Information Flyer.

HALO®-Real Time QuickGuide coming soon.

HALO®-Real Time Assay Manual coming soon.

 Related Assays

Download the HALO®-96 Research Quick Guide