Phone: (719) 264-6250    email: info@hemogenix.com    Sign In | User Pages


Products - HemoFLUOR
 
  • HemoFLUOR™ Proliferation

    Assays

  • HemoFLUOR™ PCA:

    Progenitor Cell Assays

  • HemoFLUOR™ PMT

    Patient Monitoring Assays

  • HemoFLUOR™ TE: Time

    to Engraftment Assays

HemoFLUOR™

Hematopoietic Stem and Progenitor Cell Assays. 96-well, 5-7 day, fluorescence end point proliferation and viability complete assay kits for 7 stem and 9 progenitor cell populations from 8 secies

 

GO TO Preferred Cell Systems

 

 

Compare HemoGenix and Competitor CFU Products for Research

Applications / Research Areas
  • Hematopoiesis
  • Lymphopoiesis
  • Stem cell biology
  • Cell proliferation
  • Cell viability

 

Uses of HemoFLUOR™
  • Determine viability, proliferation ability and potential.
  • Compare hematopoietic cell populations from different species.
  • Replaces hematopoietic colony-forming cell (CFC) assay applications.
  • Experimental transplantation models.
  • Effects of growth factors/cytokines.
  • In vivo to in vitro assays.
  • Gene targeting assays.

 

Benefits of using HemoFLUOR™
  • No colony counting needed.
  • Non-subjective, instrument-based and quantitative.
  • Uses a plate reader or multiparameter plate reader capable of measure fluorescence (380-400nm excitation/505nm emission).
  • Multiplexes with flow cytometry allowing cells to be detected using other fluorescent labels/markers. 
  • Multiplexes with bioluminescence assays.
  • Use in place of colorimetric/absorbance assays for greater sensitivity.
  • Results in about 7 days depending on cell population and species.
  • Complete assay kit includes cell population-specific HemoFLUOR™ Master Mix and cell processing reagent.
  • Easy to learn, fast to use and greater flexibility.
  • HemoFLUOR™-96 incorporates the same proven Suspension Expansion Culture™ (SEC™) Technology developed for HALO® bioluminescence assays available as low serum or serum-free formulations.

 Species

HemoFLUOR™-96 Research is available with a low serum formulation of SEC™ Growth Media for the following species:

  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
  • Horse
  • Pig
  • Sheep
  • Dog
  • Rat
  • Mouse

 

HemoFLUOR™-96 Research is also available with serum-free SEC™ Growth Media for:

  • Human
  • Primate
  • Mouse

 Individual and "Global" Cell Populations Detected with HemoFLUOR™-96 Research

(see Lympho-Hematopoietic System to view cell populations and growth factor/cytokine cocktails)
HemoFLUOR™-96 is available for the following cell populations:

Stem Cells (download the Stem Cell Heirarchy Population Chart)

  • HPP-SP X: IL-3, IL-6, SCF, Flt3-L (only for human cells and only serum-free)
  • HPP-SP 1: IL-3, IL-6, SCF, TPO, Flt3-L
  • HPP-SP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
  • CFC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
  • CFC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
  • CFC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
  • CFC-GEM 2: EPO, GM-CSF, IL-3, SCF
  • CFC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

 

Primitive Stem Cell-Producing, T-Lymphocyte Cell Populations

  • HPP-SP XT: IL-3, IL-6, SCF, Flt3-L + CD3 + CD28 (only for human cells and only serum-free; see also T-CFC)
  • HPP-SP 1T: IL-3, IL-6, SCF, Flt3-L + IL-2, CD3 + CD28 (only for human cells and only serum-free; see also T-CFC)

 

Progenitor Cells

  • BFU-E 1: EPO, IL-3, SCF
  • BFU-E 2: EPO alone
  • GM-CFC 1: GM-CSF, IL-3, SCF
  • GM-CFC 2: GM-CSF, G-CSF, IL-3, SCF
  • GM-CSF 3: GM-CSF alone
  • Mk-CFC 1: TPO, IL-3, SCF
  • Mk-CFC 2: TPO alone

 

Lymphopoietic cells

  • T-CFC: IL-2 alone
  • B-CFC: IL-7 alone

No growth factors: Add growth factors/cytokines of choice.

 

HemoFLUOR™-96 Research "Global" Population Assays

4-Population "Global" Assays include reagents to measure:

  • CFC-GEMM 1
  • BFU-E 1
  • GM-CFC 1
  • Mk-CFC 1
  • Background controls

 

5-Population "Global" Assays include reagents to measure:

  • HPP-SP 2
  • CFC-GEMM 1
  • BFU-E 1
  • GM-CFC 1
  • Mk-CFC 1
  • Background control

 

7-Population "Global" Assays include reagents to measure:

  • HPP-SP 2
  • CFC-GEMM 1
  • BFU-E 1
  • GM-CFC 1
  • Mk-CFC 1
  • T-CFC
  • B-CFC
  • Background control

 HemoFLUOR™-96 Research Assay Principle
  • Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
  • After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time: 2 hours.
  • Uses a plate fluorometer or multiparameter plate reader to measure fluorescence with an excitation filter of 380-400nm and an emission filter of 505nm.  

 Use and Major Equipment Required

For Research Use Only.

Major Equipment Required

A fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.

 Assay Kit Contents
Kit Contents
  • HemoFLUOR™-96 Master Mix(s) (Please choose between  low serum and serum-free formulations)
  • GF-AFC Reagent
  • Sterile 96-well culture plates
  • Sterile adhesive foil covers to keep unused wells sterile
  • Assay manual

 HemoFLUOR™-96 Research Quick Guide and Assay Manual

Download the HemoFLUOR™-96 Research Quick Guide

Please download the HemoFLUOR™-96 Research Assay Manual from the Resources page

 

HemoFLUOR™ PCA

Hematopoietic Stem and Progenitor Cell Assays. Methylcellulose-free, but MethoCult™-equivalent, 96-well, 5-day culture, assays kits for cord blood banks and cell processing laboratories

 

GO TO Preferred Cell Systems

 

 

Compare HemoGenix and Competitor CFU Products for Human Cells

Applications / Research Area
  • Hematopoiesis
  • CFU assay / Colony-forming unit assay
  • Cell therapy
  • Cell proliferation
  • Cell viability

 

Uses of HemoFLUOR™ PCA
  • A fluorescence, CFU-equivalent assay that can replace the CFU assay in cell processing laboratories.

Benefits of using HemoFLUOR™ PCA in the Cell Processing Laboratory
  • No colony counting required.
  • Transforms the subjective CFU assay into an instrument-based, quantitative assay.
  • Incorporates the same growth factor cocktails used in MethoCult® reagents for direct assay-to-assay comparisons and correlation.
  • Non-subjective, instrument-based and quantitative assay.
  • Results in 7 days.
  • Faster and easier to use than any traditional CFU assay, even using automated colony counting.
  • Complete assay kit; includes everything needed to culture and obtain results.
  • Easy to learn, fast to use and cheaper than the methylcellulose CFU assays, especially MethoCult® Express.
  • HemoFLUOR™-96 PCAEQ incorporates the same proven Suspension Expansion Culture™ (SEC™) Technology used in HALO® assays.
  • Allowed by FDA and AABB.

 Cell Populations Detected with HemoFLUOR-96 PCAEQ

HemoFLUOR™-96 PCAEQ is an instrument-based, CFU-equivalent assay with similar growth factor cocktails to MethoCult® reagents

The table below shows the HemoFLUOR™-96 PCAEQ assays that are equivalent to MethoCult® reagents used in the stem cell processing laboratory. Please click on the Catalog Number to see more information and price.

HemoFLUOR™-96 PCAEQ Equivalent Assays to MethoCult® Reagents

Assay Type Catalog Number Number of Plates/Kit

Number of Samples/Kit

Equivalent

MethoCult®

Reagent

Growth Factor Cocktail
HemoFLUOR™-96 PCAEQ

K5-PCA5-1

K5-PCA5-2

K5-PCA5-4

1

2

4

16

32

64

H4435 "Enriched" EPO, GM-CSF, G-CSF, IL-3, IL-6, SCF + TPO(#)
HemoFLUOR™-96 PCAEQ

K5-PCA1-1

K5-PCA1-2

K5-PCA1-4

1

2

4

16
32
64
H4434 "Classic" EPO, GM-CSF, IL-3, SCF
HemoFLUOR™-96 PCAEQ

K5-PCA2-1

K5-PCA2-2

K5-PCA2-4

1

2

4

16
32
64
H4034 "Optimum" EPO, GM-CSF, G-CSF,
IL-3, SCF
HemoFLUOR™-96 PCAEQ

K5-PCA3-1

K5-PCA3-2

K5-PCA3-4

1

2

4

16
32
64
H4534 "Classic" GM-CSF, IL-3, SCF
HemoFLUOR™-96 PCAEQ

K5-PCA4-1

K5-PCA4-2

K5-PCA4-4

1

2

4

16
32
64
H4035 "Optimum" GM-CSF, G-CSF, IL-3, SCF

(#) denotes that the HemoGenix® formulation of this product includes TPO that stimulates the production of megakaryopoiesis. MethoCult™ and other competitor formulations of this specific product do not include TPO, do not stimulate the production of megakaryocytes and threfore do not detect the CFC-GEMM stem cell population.

 HemoFLUOR™-96 PCAEQ Assay Principle
  • Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
  • After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time, approx. 2 hours.
  • Uses a plate fluorometer or multiparameter plate reader to measure fluorescence with an excitation filter of 380-400nm and an emission filter of 505nm.

 Tissues
  • HemoFLUOR™-96 PCAEQ can be used for the following tissues:
  • Mobilized peripheral blood
  • Umbilical cord blood
  • Bone marrow
  • Purified cell populations for any of the above tissues

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

A fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.

 Assay Kit Contents
  • HemoFLUOR™-96 PCAEQ Master Mix
  • GF-AFC reagent
  • Sterile, 96-well plate(s)
  • Sterile, adhesive foil covers so that unused wells remain sterile
  • Assay Manual
 HemoFLUOR™-96 PCAEQ Quick Guide and Assay Manual

Download the HemoFLUOR™-96 PCAEQ Quick Guide

The HemoFLUOR™-96 PCAEQ assay manual can be downloaded from the Resource page

HemoFLUOR™ PMT

"Global" Hematopoietic Stem and Progenitor Cell Assays. 5-7 day, 96-well, complete fluorescence, 4-, 5- or 7-cell population assays to monitor the patient lympho-hematopoietic system after stem cell transplantation

 

GO TO Preferred Cell Systems

 

 

Application / Research Areas
  • Patient Monitoring after Transplantation
  • Hematopoiesis
  • Cellular Therapy
  • Cell Proliferation
  • Cell Viability

 

Uses of HemoFLUOR™ PMT

Determine "global" hematopoietic and lympho-hematopoietic stem and progenitor cell reconstitution.

  • Replacement for the CFU assay for hematopoietic lineage reconstitution.

 

Benefits of using HemoFLUOR™ PMT for Patient Monitoring
  • Measurement of progenitor cell proliferation, rather than differentiation, provides faster results.
  • Measures individual hematopoietic lineages providing a faster prediction of possible reconstitution than the “classic” CFU assay.
  • No colony counting required.
  • Transforms the subjective CFU assay into a on-subjective, instrument-based, quantitative assay.
  • Easier to use than any traditional CFU assay, even using automated colony counting.
  • Complete assay kit; includes everything needed to culture and obtain results.
  • Easy to learn, fast to use and cheaper than methylcellulose CFU assays.
  • HemoFLUOR™-96 PMT incorporates the same proven Suspension Expansion Culture™ (SEC™) Technology used in HALO® assays.

 Multiple Lineages for "Global" Hematopoietic Reconstitution
  • HPP-SP, primitive stem cell
  • CFC-GEMM, hematopoietic stem cell
  • GM-CFC, myelomonocytic lineage
  • BFU-E, erythropoietic lineage
  • Mk-CFC, megakaryopoietic lineage
  • T-CFC, T-cell lineage
  • B-CFC, B-cell lineage

HemoFLUOR™-96 PMT Assays Kits Available

Assay Type Population Measured Catalog Number Number of Plates/Kit

Number of Samples/Kit

Click to see price

HemoFLUOR™-96
PMT

CFC-GEMM,

BFU-E, GM-CFC,

Mk-CFC, Control(1)

K5-4PMT-5

5

8 - 16(#)

Price
HemoFLUOR™-96
PMT

HPP-SP,

CFC-GEMM,
BFU-E, GM-CFC,
Mk-CFC, Control(2)

K5-5PMT-6

6

8 - 16(#)

Price
HemoFLUOR™-96
PMT
HPP-SP,
CFC-GEMM,
BFU-E, GM-CFC,
Mk-CFC, T-CFC, B-CFC, Control(3)

K5-7PMT-8

8

8 -16(#)

Price

(1): To predict "global" hematopoietic reconstitution.

(2): To predict "global" hematopoietic reconstitution.

(3): To predict "global" lympho-hematopoietic reconstitution.

(#): The number of samples measured/plate depends on the number of replicates plated. HemoGenix recommends a minimum of 6 and a maximum of 8 replicates. One plate is provided for each cell population measured.

 HemoFLUOR™-96 PMT Assay Principle
  • Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
  • After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time, approx. 2 hours.
  • Uses a plate fluorometer or multiparameter plate reader to measure fluorescence with an excitation filter of 380-400nm and an emission filter of 505nm.

 Tissues
  • Peripheral blood
  • Bone marrow (biopsy)

 Use and Major Equipment Required

For research use only. Not for clinical diagnostic use.

Major Equipment Required

A fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.

 HemoFLUOR™-96 PMT Assay Kit Contents
  • HemoFLUOR™-96 PMT Master Mixes for each cell population tested
  • GF-AFC Reagent
  • Sterile, 96-well plate(s)
  • Sterile, adhesive cover foils to maintain sterility of unused wells
  • Instruction manual

 HemoFLUOR™-96 PMT Quick Guide and Assay Manual

Download the HemoFLUOR™-96 PMT Quick Guide

The assay manual for HemoFLUOR™-96 PMY can be found in the Resources section here

HemoFLUOR™ TE

A 5-day complete fluorescence assay kit to determine time to engraftment of neutrophils, red blood eells and platelets after hematopoietic stem cell transplantaion

 

GO TO Preferred Cell Systems

 

 

Applications / Research Areas
  • Time to Engraftment
  • Hematopoiesis
  • Stem cells
  • Cellular Therapy
  • Cell Proliferation
  • Cell viability 

 

Uses of HemoFLUOR™ TE
  • To rapidly predict and measure the time to neutrophil, erythroid and/or platelet engraftment after hematopoietic stem cell transplantation.
  • To promote "Best Practice Criteria Testing" that supplements present "Minimal Testing Criteria".

 

Benefits of using HemoFLUOR™ TE for Detecting Time to Engraftment
  • Measure the ability and amount of GM-CFC, BFU-E and/or Mk-CFC progenitor cell proliferation prior to detecting differentiated cells in a CFU assay.
  • Proliferation of progenitor cells can be detected within 5 days.
  • Allows for the detection of individual hematopoietic progenitor cell populations or all three simultaneously after transplantation.
  • Faster, easier and more accurate to use than any traditional CFC assay, even with automated colony counting.
  • Incorporates methylcellulose-free, Suspension Expansion Culture (SEC) Technology for rapidy, accurate and cost-effective assays.
  • HemoFLUOR™-96 TE is available with low serum or serum-free formulations of HemoFLUOR™ Master Mixes.

 Cell Populations to Determine Time to Engraftment
  • BFU-E 1: Erythropoietic progenitor cell, used to predict red blood cell production.
  • GM-CFC 1: Granulocyte-macrophage progenitor cell, used to determine neutrophil engraftment.
  • Mk-CFC 1: Megkaryopoietic progenitor cell, used to predict platelet engraftment.

HemoFLUOR™-96 TE Assays Available

Assay Type Population Measured

Catalog Number

(Low serum /

Serum-Free)

Number of Plates/Kit

Number of Samples/Kit

HemoFLUOR™-96 TE

BFU-E 1

K5-BTE-1(1) /

K5-BTESF-1(2)

1

16(#)

HemoFLUOR™-96 TE

GM-CFC 1

K5-GMTE-1(1) /

K5-GMTESF-1(2)

1

16(#)

HemoFLUOR™-96 TE Mk-CFC 1

K5-MkTE-1(1) /

K5-MkTESF-1(2)

1

16(#)

HemoFLUOR™-96 TE BFU-E 1, + GM-CFC 1 + Mk-CFC 1

K5-3PTE-1(1) /

K5-3PTESF-1(2)

1

16(#)

(1): Low serum assays kits.

(2): Serum-free assay kits.

(#): These assay kits detect 16 samples for each cell population detected using 6 replicates/sample.

 Sources of Cells for Detection
  • Umbilical cord blood
  • Peripheral blood
  • Bone marrow biopsy
Tissues used for Detection
  • Peripheral blood
  • Bone marrow biopsy

 Cell Purity Requirements

The recomended cell purity is a mononuclear cell (MNC) fraction. A total nucleated cell (TNC) fraction is not recommended as this contains high concentrations of cell impurities, such as red blood cells, neutrophils, platelets and other cells that severely underestimate and even inhibit the detection of progenitor cells. 

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Luminescence plate reader or multiparameter plate reader with "glow" luminescence measuring capability.

 HemoFLUOR™-96 TE Assay Kit Contents
  • HemoFLUOR™-96 TE Master Mix(s) as either low serum or serum-free formulations
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

 HemoFLUOR™-96 TE Quick Guide and Assay Manual

Download the HemoFLUOR™-96 Quick Guide

The HemoFLUOR™-96 TE Assay Manual can be found in the Resources section here