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Products - HemoLIGHT
 
  • HemoLIGHT™-96:

    Absorbance Proliferation Assays

  • HemoLIGHT™-96 PCAEQ:

    CFU-Equivalent Assays

  • HemoLIGHT™-96 PMT:

    Patient Monitoring Assays

  • HemoLIGHT™-96 TE:

    Time to Engraftment

HemoLIGHT96

HemoLIGHT™-96 Research

Hematopoietic Stem and Progenitor Cell Assays. 96-well, 5-7 day, absorbance proliferation and viability complete assay kits for 7 stem and 9 progenitor cell populations from 8 species

 

Buy HemoLIGHT-96 Research Assays

 

 

Compare HemoGenix and Competitor CFU Products for Research

Applications / Research Areas
  • Hematopoiesis
  • Lymphopoiesis
  • Stem cell research
  • Cell proliferation
  • Cell Viability
 
Uses of HemoLIGHT™-96 Research
  • Determine viability, proliferation ability and potential.
  • Compare hematopoietic cell populations from different species.
  • Replaces hematopoietic colony-forming cell (CFC) assay applications.
  • Experimental transplantation models.
  • Effects of growth factors/cytokines.
  • In vivo to in vitro assays.
  • Gene targeting assays.

 

Benefits of using HemoLIGHT™-96 for your Research
  • Uses a colorimetric/absorbance ELISA plate reader, availble in most laboratories.
  • No colony counting required.
  • Non-subjective, instrument-based and quantitative.
  • Results in about 7 days depending on species.
  • Complete assay kit; includes everything needed to culture and measure hematopoietic cell growth.
  • Easy to learn, fast to use and cheaper than the methylcellulose CFC assay.
  • HemoLIGHT™ incorporates the same proven Suspension Expansion Culture™ (SEC™) Technology developed for HALO® bioluminescence assays.

 Species

HemoLIGHT™-96 Research is available with a low serum formulation of SEC™ Growth Media for the following species:

  • Human
  • Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
  • Horse
  • Pig
  • Sheep
  • Dog
  • Rat
  • Mouse

 

HemoLIGHT™-96 Research is also available with serum-free SEC™ Growth Media for:

  • Human
  • Primate
  • Mouse

 Individual and "Global" Cell Populations Detected using HemoLIGHT™-96

(see Lympho-Hematopoietic System to view cell populations and growth factor/cytokine cocktails)
HemoLIGHT™-96 is available for the following cell populations:

Stem Cells (download the Stem Cell Population Heirarchy Chart)

  • HPP-SP X: IL-3, IL-6, SCF, Flt3-L (only for human cells and only serum-free)
  • HPP-SP 1: IL-3, IL-6, SCF, TPO, Flt3-L
  • HPP-SP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
  • CFC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
  • CFC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
  • CFC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
  • CFC-GEM 2: EPO, GM-CSF, IL-3, SCF
  • CFC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

 

Primitive Stem Cell-Producing, T-Lymphocyte Cell Populations

  • HPP-SP XT: IL-3, IL-6, SCF, Flt3-L + CD3 + CD28 (only for human cells and only serum-free; see also T-CFC)
  • HPP-SP 1T: IL-3, IL-6, SCF, Flt3-L + IL-2, CD3 + CD28 (only for human cells and only serum-free; see also T-CFC)

 

Progenitor Cells

  • BFU-E 1: EPO, IL-3, SCF
  • BFU-E 2: EPO alone
  • GM-CFC 1: GM-CSF, IL-3, SCF
  • GM-CFC 2: GM-CSF, G-CSF, IL-3, SCF
  • GM-CSF 3: GM-CSF alone
  • Mk-CFC 1: TPO, IL-3, SCF
  • Mk-CFC 2: TPO alone

 

Lymphopoietic cells

  • T-CFC: IL-2 alone
  • B-CFC: IL-7 alone

No growth factors: Add growth factors/cytokines of choice.

 

HemoLIGHT™-96 Research "Global" Population Assays

4-Population "Global" Assays include reagents to measure:

  • CFC-GEMM 1
  • BFU-E 1
  • GM-CFC 1
  • Mk-CFC 1
  • Background controls

 

5-Population "Global" Assays include reagents to measure:

  • HPP-SP 2
  • CFC-GEMM 1
  • BFU-E 1
  • GM-CFC 1
  • Mk-CFC 1
  • Background control

 

7-Population "Global" Assays include reagents to measure:

  • HPP-SP 2
  • CFC-GEMM 1
  • BFU-E 1
  • GM-CFC 1
  • Mk-CFC 1
  • T-CFC
  • B-CFC
  • Background control

 The HemoLIGHT™-96 Principle
  • Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction, colorimetric reaction.
  • After culture, just add 20μl of the MTS reagent, mix and read absorbance at 490nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.
  • Read results between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.

 Use and Major Equipment Required

For Research Use Only.

Major Equipment Required

An absorbance plate readr or multiparameter plate reader to measure absorbance at 490nm.

 Assay Kit Contents
  • HemoLIGHT™-96 Master Mix(s). (Please choose between low serum and serum-free formulations)
  • Sterile 96-well culture plates
  • Sterile adhesive foil covers to keep unused wells sterile.
  • Assay manual

 HemoLIGHT™-96 Research Quick Guide and Assay Manual

Download the HemoLIGHT™-96 Research Quick Guide

The HemoLIGHT™-96 Research Assay Manual can be found in the Resources section here

HemoLIGHT-96 PCAeq: An advanced absorbance/colorimetric progenitor cell equivalent replacement assay for the CFU assay

HemoLIGHT™-96 PCAEQ

Hematopoietic Stem and Progenitor Cells. Methylcellulose-free, but MethoCult™-equivalent, 96-well, 5-day culture assay kits for cord blood banks and cell processing laboratories

 

Buy HemoLIGHT-PCAEQ assay kits or click on the Catalog No. below

 

 

Compare HemoGenix and Competitor CFU Products for Human Cells

Application / Research Areas
  • Hematopoiesis
  • CFU Assay / Colony-Forming Unit Assay
  • Cellular Therapy
  • Cell proliferation
  • Cell Viability
 
Uses of HemoLIGHT™-96 PCAEQ
  • Determine the number of viable cells in proliferation.
  • An alternative to, or replacement for the classic colony-forming unit (CFU) assay for stem cell processing laboratories.
  • FDA, FACT and AABB alternative assays. (NMDP uses FACT and AABB standards).

Benefits of using HemoLIGHT™-96 PCAEQ in the Cell Processing Laboratory
  • No colony counting required.
  • Replaces the CFU assay with an instrument-based, quantitative assay.
  • Uses an absorbance ELISA plate reader available in most laboratories.
  • Incorporates the same growth factor cocktails used in MethoCult® reagents for direct assay-to-assay comparisons and correlation.
  • Non-subjective, instrument-based and quantitative assay system.
  • Results in 7 days depending.
  • Faster and easier to use than any traditional CFU assay, even using automated colony counting.
  • Cheaper than the methylcellulose CFU assays.
  • Complete assay kit; includes everything needed to culture and obtain results.
  • Flexibility to obtain results depending on work schedule.
  • HemoLIGHT™-96 PCAEQ incorporates the same proven Suspension Expansion Culture™ (SEC™) Technology used in HALO® assays.

 HemoLIGHT™-96 PCAEQ is a CFU-Equivalent Assay

HemoLIGHT™-96 PCAEQ is a CFU-Equivalent Assay that uses the same growth factor cocktails MethoCult®

The table below shows the HemoLIGHT™-96 PCAEQ assays that are equivalent to CAMEO™-4 assays and MethoCult reagents used in the stem cell processing laboratory. The growth factor/cytokine cocktail is the same for all three.

HemoLIGHT™-96 PCAEQ Equivalent Assays to MethoCult® Reagents

Assay Type Catalog Number Number of Plates/Kit

Number of Samples/Kit

Equivalent

MethoCult

Reagent

Growth Factor Cocktail
HemoLIGHT™-96 PCAEQ

K4-PCA5-1

K4-PCA5-2

K4-PCA5-4

1

2

4

16

32

64

H4435 "Enriched" EPO, GM-CSF, G-CSF, IL-3, IL-6, SCF + TPO(#)
HemoLIGHT™-96 PCAEQ

K4-PCA1-1

K4-PCA1-2

K4-PCA1-4

1

2

4

16
32
64
H4434 "Classic" EPO, GM-CSF, IL-3, SCF
HemoLIGHT™-96 PCAEQ

K4-PCA2-1

K4-PCA2-2

K4-PCA2-4

1

2

4

16
32
64
H4034 "Optimum" EPO, GM-CSF, G-CSF,
IL-3, SCF
HemoLIGHT™-96 PCAEQ

K4-PCA3-1

K4-PCA3-2

K4-PCA3-3

1

2

4

16
32
64
H4534 "Classic" GM-CSF, IL-3, SCF
HemoLIGHT™-96 PCAEQ

K4-PCA4-1

K4-PCA4-2

K4-PCA4-4

1

2

4

16
32
64
H4035 "Optimum" GM-CSF, G-CSF, IL-3, SCF

(#) denotes that the HemoGenix® formulation of this product includes TPO that stimulates the production of megakaryopoiesis. MethoCult™ and other competitor formulations of this specific product do not include TPO, do not stimulate the production of megakaryocytes and threfore do not detect the CFC-GEMM stem cell population.

*denotes that assay kits are available with 1, 2 or 4, 96-well plates

 HemoLIGHT™-96 PCAEQ Principle
  • Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction, colorimetric reaction.
  • After culture, just add 20μl of the MTS reagent, mix and read absorbance at 490nm after 1-4 hours in a 96-well plate reader.
  • No solubilization step as in a MTT reaction.
  • Read results between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.

 Tissues

HemoLIGHT-96 PCAEQ can be used for the following tissues:

  • Mobilized peripheral blood
  • Umbilical cord blood
  • Bone marrow
  • Purified cell populations for any of the above tissues

 Use and Major Equipment required
For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required
An absorbance plate readr or multiparameter plate reader to measure absorbance at 490nm.

 Assay Kit Contents
  • HemoLIGHT™-96 PCAEQ Master Mix
  • MTS reagent
  • Sterile, 96-well plate(s)
  • Sterile, adhesive cover foils to maintain sterility of unused wells
  • Instruction manual

 HemoLIGHT™-96 PCAEQ Quick Guide and Assay Manual

Download the HemoLIGHT™-96 PCAEQ Quick Guide

Access the HemoLIGHT™-96 PCAEQ on the Resources page here

HemoLIGHT-96 PMT: A "global" patient monitoring after stem cell transplantation of 4-, 5- or 7-cell populations

HemoLIGHT™-96 PMT

"Global" Hematopoietic Stem and Progenitor Cell Assay Kits. 5-7 day, 96-well, complete absorbance, 4-, 5- or 7-cell population assays to monitor the patient lympho-hematopoietic system after stem cell transplantation

 

Buy HemoLIGHT-96 PMT assay kits or click on the Price below

 

 

 

Applications / Research Areas
  • Hematopoiesis
  • Patient monitoring after transplantation
  • Stem cells 
  • Cell proliferation
  • Cell viability

 

Uses of HemoLIGHT™-96 PMT

Determine "global" hematopoietic stem and progenitor cell reconstitution.

  • An alternative to, or replacement for the classic colony-forming unit (CFU) assay for hematopoietic lineage engraftment and reconstitution.

Benefits of using HemoLIGHT™-96 PMT in the Cell Processing Laboratory
  • Measurement of progenitor cell proliferation, rather than differentiation, provides faster results.
  • Measures individual hematopoietic lineages providing a faster prediction of possible reconstitution than the “classic” CFU assay.
  • No colony counting required.
  • Results in 7 days.
  • Transforms the subjective CFU assay into an instrument-based, quantitative assay.
  • Easier to learn and faster to use than any traditional CFU assay, even using automated colony counting.
  • Complete assay kit; includes everything needed to culture and measure hematopoietic cell growth.
  • HemoLIGHT™-96 PMT incorporates the same proven Suspension Expansion Culture™ (SEC™) Technology used in HALO® assays.

 Multiple Lineages for "Global" Hematopoietic Reconstitution
  • HPP-SP, primitive stem cell
  • CFC-GEMM, hematopoietic stem cell
  • GM-CFC, myelomonocytic lineage
  • BFU-E, erythropoietic lineage
  • Mk-CFC, megakaryopoietic lineage

HemoFLUOR™-96 PMT Assays Kits Available

Assay Type Population Measured Catalog Number Number of Plates/Kit

Number of Samples/Kit

Click to see price

HemoLIGHT™-96
PMT

CFC-GEMM,

BFU-E, GM-CFC,

Mk-CFC, Control(1)

K4-4PMT-5

5

8 - 16(#)

Price
HemoLIGHT™-96
PMT

HPP-SP,

CFC-GEMM,
BFU-E, GM-CFC,
Mk-CFC, Control(2)

K4-5PMT-6

6

8 - 16(#)

Price
HemoLIGHT™-96
PMT
HPP-SP,
CFC-GEMM,
BFU-E, GM-CFC,
Mk-CFC, T-CFC, B-CFC, Control(3)

K4-7PMT-8

8

8 -16(#)

Price

(1): To predict "global" hematopoietic reconstitution.

(2): To predict "global" hematopoietic reconstitution.

(3): To predict "global" lympho-hematopoietic reconstitution.

(#): The number of samples measured/plate depends on the number of replicates plated. HemoGenix recommends a minimum of 6 and a maximum of 8 replicates. One plate is provided for each cell population measured.

 HemoLIGHT™-96 PMT Principle

  • Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction, colorimetric reaction.
  • After culture, just add 20μl of the MTS reagent and read absorbance at 490nm after 1-4 hours in a 96-well plate reader.
  • Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction.

 Tissues
  • Peripheral blood
  • Bone marrow (biopsy)

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

An absorbance plate readr or multiparameter plate reader to measure absorbance at 490nm.

 Assay Kit Contents
  • HemoLIGHT™-96 PMT Master Mix
  • MTS reagent
  • Sterile, 96-well plate(s)
  • Sterile adhesive cover foils for maintaining unused well sterile
  • Instruction manual

 HemoLIGHT™-96 PMT Quick Guide and Assay Manual

Download the HemoLIGHT™-96 PMT Quick Guide

The HemoLIGHT™-96 PMT Assay Manual can be accessed in the Resources section here

HemoLIGHT-96 TE: Hematopoietic stem cell time to engraftment assay

HemoLIGHT™-96 TE

5-day complete absorbance assay kits to determine time to engraftment of neutrophils, red blood cells and platelets after hematopoietic stem cell transplantaion

 

Buy HemoLIGHT™-96 TE assay kits

 

 

Applications / Research Areas
  • Hematopoiesis
  • Time to engraftment after transplantation
  • Cellular therapy
  • Cell proliferation
  • Cell viability
 
Uses of HemoLIGHT™-96 TE
  • To rapidly predict and measure the time to neutrophil, erythroid and/or platelet engraftment after hematopoietic stem cell transplantation.
  • To promote "Best Practice Criteria Testing" that supplements present "Minimal Testing Criteria".

 

Benefits of using HemoLIGHT™-96 TE for Detecting Time to Engraftment
  • Measure the ability and amount of GM-CFC, BFU-E and/or Mk-CFC progenitor cell proliferation prior to detecting differentiated cells in a CFU assay.
  • Proliferation of progenitor cells can be detected within 5 days.
  • Allows for the detection of individual hematopoietic progenitor cell populations or all three simultaneously after transplantation.
  • Faster, easier and more accurate to use than any traditional CFC assay, even with automated colony counting.
  • Incorporates methylcellulose-free, Suspension Expansion Culture (SEC) Technology to produce rapid, accurate and cost-effective assays.
  • HemoFLUOR™-96 TE is available with low serum or serum-free formulations of HemoFLUOR™ Master Mixes.

 Cell Populations Detected

To determine Time to Engraftment:

  • BFU-E 1: Erythropoietic progenitor cell, used to predict red blood cell production.
  • GM-CFC 1: Granulocyte-macrophage progenitor cell, used to determine neutrophil engraftment.
  • Mk-CFC 1: Megkaryopoietic progenitor cell, used to predict platelet engraftment.

HemoLIGHT™-96 TE Assays Available

Assay Type Population Measured

Catalog Number

(Low serum /

Serum-Free)

Number of Plates/Kit

Number of Samples/Kit

HemoLIGHT™-96 TE

BFU-E 1

K4-BTE-1(1) /

K4-BTESF-1(2)

1

16(#)

HemoLIGHT™-96 TE

GM-CFC 1

K4-GMTE-1(1) /

K4-GMTESF-1(2)

1

16(#)

HemoLIGHT™-96 TE Mk-CFC 1

K4-MkTE-1(1) /

K4-MkTESF-1(2)

1

16(#)

HemoLIGHT™-96 TE BFU-E 1, + GM-CFC 1 + Mk-CFC 1

K4-3PTE-1(1) /

K4-3PTESF-1(2)

1

16(#)

(1): Low serum assays kits.

(2): Serum-free assay kits.

(#): These assay kits detect 16 samples for each cell population detected using 6 replicates/sample.

 Hematopoietic Stem Cell Product Transplantation Sources
  • Umbilical cord blood
  • Peripheral blood
  • Bone marrow biopsy

Tissues used for Detection
  • Peripheral blood
  • Bone marrow biopsy

 Cell Purity Requirements for Detection

The recomended cell purity is a mononuclear cell (MNC) fraction. A total nucleated cell (TNC) fraction is not recommended as this contains high concentrations of cell impurities, such as red blood cells, neutrophils, platelets and other cells that severely underestimate and even inhibit the detection of progenitor cells. 

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Luminescence plate reader or multiparameter plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
  • HemoLIGHT™-96 TE Master Mix(s) as either low serum or serum-free formulations
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

 HemoLIGHT™-96TE Quick Guide and Assay Manual

Download the HemoLIGHT™-96 TE Quick Guide

The HemoLIGHT™-96 TE Assay Manual can be found in the Resources section here