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Products - ImmunoFLUOR™
 
  •  ImmunoFluor™: Lymphocyte

    Proliferation Assays

  • ImmunoFluor™ MLC: Mixed

    Lymphocyte Culture

ImmunoFluor™

ImmunoFluor™ TCP

 

Fast, Reliable, 96-Well Fluorescence Complete Assay Kit for Immune Cell Functionality and Lymphocyte Proliferation with Multiplexing Capability

 

Buy ImmunoFLUOR™ Assay Kits

 

Buy ImmunoFLUOR™ TCP Assay Kits

 

 

 
Uses of ImmunoFluor™ and ImmunoFluor™ TCP
  • Lymphocyte activation.
  • T-lymphocyte proliferation (ImmunoFluor™ TCP)
  • Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
  • Cellular immune response studies.
  • Single-cell, T-lymphocyte cloning studies.
  • Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
  • Effect of accessory (non T-cells) on T cell induction.
  • Effect of co-stimulators on T-cell induction.
  • Effect of epitope sequences and novel peptides or proteins.
  • Test the response of primed T-cells in vitro.

 

Benefits of using ImmunoFluor™-96 and ImmunoFluor™-96 TCP
  • Non-subjective, instrument-based and quantitative.
  • Multiplexes with flow cytometry protocols using other fluorescent labels, e.g. FITC, PE, PECy5, PerCP-Cy5, PE-Cy7 etc.
  • Multiplexes with bioluminescence assays.
  • Use in place of colorimetric/absorbance assays for greater sensitivity.
  • Assay kit includes supplies for culture and cell processing.
  • Easy to learn, fast to use.

 Species

ImmunoFluor™-96 can be used to study lymphocytes from many species including, but not limited to:

  • Human
  • Non-human primate
  • Mouse

 Tissues
  • Peripheral blood mononuclear cells (PBMC)
  • Lymphocyte sub-populations
  • Lymphocyte tissues and organs

 ImmunoFluor™-96 and ImmunoGlo™-96 TCP Assays Available

ImmunoFluor™-96 Assays Available

Assay Type Catalog Number Number of Plates/Kit

Number of Samples/Kit(#)

ImmunoFluor™-96

KM2-LPA-1

1

16 - 24

ImmunoFluor™-96

KM2-LPA-2

2

32 - 48

ImmunoFluor™-96

KM2-LPA-4

4

64 - 96

 (#): HemoGenix® recommends a minimum of 6 replicates/sample. However, this can be reduced to 4 replicates/sample

 

ImmunoFluor™-96 TCP

T-Cell Proliferation Assays for Human Cells

Assay Type Catalog Number HemoGro™ Serum-Free containing:

Number of Plates/Samples per Kit

ImmunoFluor™-96 TCP

KM2-1-1H

IL-2 alone

1 / 16-24

ImmunoFluor™-96 TCP

KM2-TCS1-1H

CD3 + CD28

1 / 16-24

ImmunoFluor™-96 TCP

KM2-TCS2-1H

IL-2 + CD3 + CD28

1 / 16-24

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Fluorescence plate reader or multiparameter plate reader with an excitation filter of 390-400nm and emission filter of 505nm.

 Assay Kit Contents
  • ImmunoFluor™-96 Assay Kits
  • GF-AFC Reagent
  • Sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Assay manual

ImmunoFluor™-96 TCP Assay Kits
  • T-Cell Culture and Expansion Master Mix in Serum-Free ImmunoGro™ Medium
  • GF-AFC Reagent
  • Sterile, 96-well culture plates
  • Sterile, adhesive plate cover foils to maintain sterility of unused wells
  • Assay manual

 ImmunoFluor™-96 and TCP Information Flyer, QuickGuide and Assay Manual

ImmunoFluor™--96 Information Flyer

ImmunoFluor™-96 TCP Information Flyer

ImmunoFluor™-96 Quick Guide

ImmunoFluor™-96 and TCP Assay Manual. Can also be found on the Resource Page

ImmunoFluor™ MLC

Fast and Reliable, 96-Well Fluorescence, 1- or 2-Way Mixed Lymphocyte Culture (MLC) / Reaction (MLR) Assay Kit

 

Buy ImmunoFLUOR™ MLC Assay Kits

 

 

  

Uses of ImmunoFluor™ MLC
  • One-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Two-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Allogeneic mixed lymphocyte culture.
  • Cellular immune response.
  • Dendritic cell interactions.
  • Mesenchymal stem/stromal cell interactions.
  • Agents that activate/suppress MLC/MLR.

 

Benefits of using ImmunoFluor™ MLC
  • Non-subjective, instrument-based and quantitative.
  • Multiplexes with flow cytometry protocols using other fluorescent labels, e.g. FITC, PE, PECy5, PerCP-Cy5, PE-Cy7 etc.
  • Multiplexes with bioluminescence assays.
  • Use in place of colorimetric/absorbance assays for greater sensitivity.
  • Assay kit includes supplies for culture and cell processing.
  • Easy to learn, fast to use.
 Species
  • Applicable to multiple species
 Tissues
  • Lymphocytes and lymphocyte sub-populations from a variety of sources.
  • Lymphocyte cell lines.
  • Other potential immunoreactive cell types.
 ImmunoFluor™-MLC Assays Available

ImmunoFluor™-MLC Assays Available

Assay Type Catalog Number Number of Plates/Kit

Number of Samples/Kit(#)

Click to see price

ImmunoFluor™-MLC

KM2-MLC-1

1

16 - 24

Price
ImmunoFluor™-MLC

KM2-MLC-2

2

32 - 48

Price
ImmunoFluor™-MLC

KM2-MLC-4

4

64 - 96

Price

 (#): HemoGenix® recommends a minimum of 6 replicates/sample. However, this can be reduced to 4 replicates/sample

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Fluorescence plate reader or multiparameter plate reader with an excitation filter of 390-400nm and an emission filter at 505nm.

 Assay Kit Contents
  • Mitomycin-C for one-way MLC/MLR
  • GF-AFC Reagent
  • Sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Assay manual
 ImmunoFluor™-MLC Quick Guide and Assay Manual

Download the ImmunoFluor™-MLC Quick Guide

The ImmunoFluor™-MLC Assay Manual can be found in the Resources section here