ImmunoFLUOR™-96

Fluorescence Assays for Immune Cells

Buy ImmunoFLUOR™-96 Products

Available as assay kits or
contract research services

ImmunoFLUOR™-96 Applications

• Lymphocyte activation.
• Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
• Cellular immune response studies.
• Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
• Effect of accessory (non T-cells) on T cell induction.
• Effect of co-stimulators on T-cell induction.
• Effect of epitope sequences and novel peptides or proteins.
• Test the response of primed T-cells in vitro.

Advantages of using ImmunoFLUOR™-96

• Non-subjective, instrument-based and quantitative.
• Multiplexes with flow cytometry protocols using other fluorescent labels, e.g. FITC, PE, PECy5, PerCP-Cy5, PE-Cy7 etc.
• Multiplexes with bioluminescence assays.
• Use in place of colorimetric/absorbance assays for greater sensitivity.
• Assay kit includes supplies for culture and cell processing.
• Easy to learn, fast to use.

Species

ImmunoFLUOR™-96 can be used to study lymphocytes from many species including, but not limited to:

• Human
• Non-human primate
• Mouse

Tissues

• Peripheral blood mononuclear cells (PBMC)
• Lymphocyte sub-populations
• Lymphocyte tissues and organs

Use

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Fluorescence plate reader or multiparameter plate reader with an excitation filter of 390-400nm and emission filter of 505nm.

Kit Contents

• GF-AFC Reagent
• Sterile, 96-well plates
• Sterile, adhesive foil covers
• Instruction manual

Related Products

• ImmunoGlo™-96
• ImmunoGlo™-MLC
• ImmunoLIGHT™-96
• HALO®-96 Research (for stem cells and lymphocyte progenitor cells)
• CAMEO™-4 (for stem cells and lymphocyte progenitor cells)
• CAMEO™-96 (for stem cells and lymphocyte progenitor cells)

ImmunoFLUOR™-MLC

Fluorescence Mixed Lymphocyte Culture (MLC) / Reaction (MLR) Assays

Buy ImmunoFLUOR™-MLC Products

Available as assay kits or
contract research services

ImmunoFLUOR™-MLC Applications

• One-way mixed lymphocyte culture (MLC) / reaction (MLR).
• Two-way mixed lymphocyte culture (MLC) / reaction (MLR).
• Allogeneic mixed lymphocyte culture.
• Cellular immune response.
• Dendritic cell interactions.
• Mesenchymal stem/stromal cell interactions.
• Agents that activate/suppress MLC/MLR.

Advantages of using ImmunoFLUOR™-MLC

• Non-subjective, instrument-based and quantitative.
• Multiplexes with flow cytometry protocols using other fluorescent labels, e.g. FITC, PE, PECy5, PerCP-Cy5, PE-Cy7 etc.
• Multiplexes with bioluminescence assays.
• Use in place of colorimetric/absorbance assays for greater sensitivity.
• Assay kit includes supplies for culture and cell processing.
• Easy to learn, fast to use.

Species

• Applicable to multiple species

Tissues

• Lymphocytes and lymphocyte sub-populations from a variety of sources.
• Lymphocyte cell lines.
• Other potential immunoreactive cell types.

Use

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Fluorescence plate reader or multiparameter plate reader with an excitation filter of 390-400nm and an emission filter at 505nm.

Kit Contents

• Mitomycin-C for one-way MLC/MLR
• GF-AFC Reagent
• Sterile, 96-well plates
• Sterile, adhesive foil covers
• Instruction manual

Related Products

• ImmunoGlo™-MLC
• ImmunoLIGHT™-MLC
• ImmunoGlo™-96
• ImmunoFLUOR™-96
• ImmunoLIGHT™-96