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Products - ImmunoGlo
 
  •  ImmunoGlo™: Lymphocyte

    Proliferation Assays

  •  ImmunoGlo™ MLC: Mixed

    Lymphocyte Culture

  •  ImmunoGlo™-Tox HT:

    Immunotoxicity Assays

ImmunoGlo™

ImmunoGlo™ TCP 

ImmunoGlo™ Real Time

 

Reliable, Standardized and Validated, 96-Well Bioluminescence Complete Assay Kit for Immune Cell Functionality and Lymphocyte Proliferation

 

Buy ImmunoGlo™ Assay Kits

 

Buy ImmunoGlo™ TCP Assay Kits

 

Buy ImmunoGlo™ Real Time Assay Kits

 

 
Uses of ImmunoGlo™-96 and ImmunoGlo-96 TCP
  • Lymphocyte activation.
  • T-lymphocyte proliferation (ImmunoGlo™-96 TCP)
  • Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
  • Cellular immune response studies.
  • Single-cell, T-lymphocyte cloning studies.
  • Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
  • Effect of accessory (non T-cells) on T cell induction.
  • Effect of co-stimulators on T-cell induction.
  • Effect of epitope sequences and novel peptides or proteins.
  • Test the response of primed T-cells in vitro.
  • In vitro immunotoxicity studies. (See also ImmunoGlo™-384 HT).
  • Immune cell gene targeting and expression, e.g. CRISPR-Cas9.

 

Benefits of using ImmunoGlo™96 and ImmunoGlo™-96 TCP
  • For all assays involving lymphocyte proliferation.
  • Non-radioactive assay incorporating proven ATP bioluminescence technology.
  • Instrument-based, quantitative and fully standardized allowing comparison of results over time.
  • 10 - 100 times more sensitive than WST-1, CSFE an other absorbance or fluorescence assays.
  • Available in 96- or 384-well plate formats for high throughput toxicity studies. (See ImmunoGlo™-384 HT).
  • Complete flexibility to use any mitogens, co-stimulators etc and lymphocyte culture protocols.
  • Multiplexing capability to incorporate flow cytometry, cytokine release and other assay readouts for additional information from the same sample.

 

ImmunoGlo™-Real Time

ImmunoGlo™-Real Time allows immune cell proliferation or immune cytotoxicity to be viewed as the cells are actually growing or are being inhibited from growth under different conditions. The ImmunoGlo™-Real Time reagents can be added at the start of immune cell culture or at any time after culture initiation. After stabilization of the cells with the reagent from 2-3 hours in the incubator, the 96-well culture plate can be transiently removed from the incubator and the, the luminescence measured and then replaced. Measurement of luminescence can be performed at any time over a 2-3 day period producing a growth/cytotoxicity curve from which the cell parameters, such as cell doubling time, can be determined.

ImmunoGlo™-Real Time is a non-lytic, non-destructive assay, thereby allowing other endpoints, flow cytometry and cell phenotyping, for example, to be performed directly on the cells in the wells.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species

ImmunoGlo™-96 can be used to study lymphocytes from many different species, including, but not limited to:

  • Human (only for ImmunoGlo-96 TCP)
  • Non-human primate
  • Dog
  • Rat
  • Mouse
 Tissues
  • Peripheral blood mononuclear cells (PBMC).
  • Lymphocyte sub-populations.
  • Lymphocyte tissues and organs
 ImmunoGlo™-96, ImmunoGlo™-96 TCP and ImmunoGlo™-Real Time Assays Available

ImmunoGlo™-96 Assays Available

Assay Type Catalog Number Number of Plates/Kit

Number of Samples/Kit(#)

ImmunoGlo™-96

KM1-LPA-1

1

16 - 24

 (#): HemoGenix® recommends a minimum of 6 replicates/sample. However, this can be reduced to 4 replicates/sample

 

ImmunoGlo™-96 TCP

T-Cell Proliferation Assays for Human Cells

Assay Type Catalog Number HemoGro™ Serum-Free containing:

Number of Plates/Samples per Kit

ImmunoGlo™-96 TCP

KM1-T-1H

IL-2 alone

1 / 16-24

ImmunoGlo™-96 TCP

KM1-TCS1-1H

CD3 + CD28

1 / 16-24

ImmunoGlo™-96 TCP

KM1-TCS2-1H

IL-2 + CD3 + CD28

1 / 16-24

 

ImmunoGlo™-Real Time

Assay Type Catalog Number Number of Plates/Kit

Number of Samples/Kit(#)

ImmunoGlo™-96

KM1-RT-1

1

16 - 24

 (#): HemoGenix® recommends a minimum of 6 replicates/sample. However, this can be reduced to 4 replicates/sample

 

 Examples Using ImmunoGlo™-96 TCP

T-lymphocyte cell production using ImmunoGlo-96 TCP assay kits

Click image for larger view

Measuring T-Lymphocyte Proliferation using ImmunoGlo™-96 TCP

In this example, human bone marrow mononuclear cells were stimulated with IL-2 alone, CD3 + CD28 or with IL-2 + CD3 + CD28 for 5 days. T-cell proliferation was measured using a standardized ATP bioluminescence readout. Flow cytometric phenotypic analysis of the IL-2+CD3+CD28 sample produced about 40% CD3/CD4, 26% CD3/CD8 and 6% CD4/CD8. 

 

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Plate luminometer or multimode plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
ImmunoGlo™-96 Assay Kits
  • Medium to dilute ATP standards
  • ATP standards
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well culture plates
  • Non-sterile, 96-well plates for ATP standard curve
  • Sterile, adhesive plate cover foils to maintain sterility of unused wells
  • Assay manual

ImmunoGlo™-96 TCP Assay Kits
  • T-Cell Culture and Expansion Master Mix in Serum-Free ImmunoGro™ Medium
  • Medium to dilute ATP standards
  • ATP standards
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well culture plates
  • Non-sterile, 96-well plate for ATP standard curve
  • Sterile, adhesive plate cover foils to maintain sterility of unused wells
  • Assay manual

ImmunoGlo™-Real Time
  • ImmunoGro™ Medium
  • Real Time reagent buffer
  • Real Time bioluminescence reagent
  • Sterile 96-well plate
  • Sterile, adhesive plate cover foils to maintain sterility of unused wells
  • Assay manual
  • Instruction manual
 ImmunoGlo™-96 and TCP Information Flyers, Quick Guide and Assay Manual


ImmunoGlo™ MLC

Reliable, Standardized and Validated, 96-Well Bioluminescence, 1- or 2-Way Mixed Lymphocyte Culture (MLC) / Reaction (MLR) Assay Kit 

 

Buy ImmunoGlo™ MLC Assay Kits

 

 

  

Uses of ImmunoGlo™ MLC
  • One-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Two-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Allogeneic mixed lymphocyte culture.
  • Cellular immune response.
  • Dendritic cell interactions.
  • Mesenchymal stem/stromal cell interactions.
  • Agents that activate/suppress MLC/MLR.

 

Benefits of using ImmunoGlo™ MLC
  • Non-radioactive assay incorporating proven ATP bioluminescence technology, to replace all radioactive MLC/MLR assays.
  • Instrument-based, quantitative and fully standardized readout procedure.
  • 10 - 100 times more sensitive than WST-1, CSFE an other absorbance or fluorescence assays.
  • Complete flexibility to use virtually any MLC/MLR protocol.
  • Reliably compare results over time.
  • Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species
  • Applicable to multiple species.

 Tissues
  • Lymphocytes and lymphocyte sub-populations from a variety of sources.
  • Lymphocyte cell lines.
  • Other potential immunoreactive cell types.

 Types of ImmunoGlo™-MLC Assays Available

ImmunoGlo™-MLC Assays Available

Assay Type Catalog Number Number of Plates/Kit

Number of Samples/Kit(#)

Click to see price

ImmunoGlo™-MLC

KM1-MLC-1

1

16 - 24

Price
ImmunoGlo™-MLC

KM1-MLC-2

2

32 - 48

Price
ImmunoGlo™-MLC

KM1-MLC-4

4

64 - 96

Price

 (#): HemoGenix® recommends a minimum of 6 replicates/sample. However, this can be reduced to 4 replicates/sample

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
  • Mitomycin-C for one-way MLC/MLR
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well culture plate(s)
  • Non-sterile, 96-well plate(s)
  • Sterile adhesive plate cover foils to maintain sterility of unused wells
  • Instruction manual. 

 ImmunoGlo™-MLC Quick Guide and Assay Manual

Download the ImmunoGlo™-MLC Quick Guide

The ImmunoGlo™-MLC Assay Manual can be accessed in the Resource section here

ImmunoGlo™-Tox HT

A Standardized and Validated 96- and 384-Well Bioluminescence Toxicity Screening Assay Platform with Multiplexing Capability for Immune Cells 

 

View ImmunoGlo™-Tox HT assay kits

 

ImmunoGlo™-Tox HT Contract Services

 

 

 
Uses of ImmunoGlo™-Tox HT
  • In vitro high-throughput immunotoxicity screening.
  • In vitro high throughput lymphocyte proliferation screening.
  • Cellular immune response screening.
  • Development of drugs and immunotherapies using gene targeting, e.g. CRISPR-Cas9.

 

Benefits of using ImmunoGlo™-Tox HT

  • Non-radioactive assay incorporating proven high-throughput ATP bioluminescence technology.
  • Instrument-based, quantitative and fully standardized allowing comparison of results over time.
  • Can be validated for GLP studies.
  • More sensitive than WST-1, CSFE an other absorbance or fluorescence assays.
  • Available in 96- or 384-well plate formats.
  • Multiplexing capability to incorporate flow cytometry, cytokine release and other assay readouts for additional information from the same sample.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species
  • ImmunoGlo™-Tox HT can be used for many different species.
 Tissues
  • Peripheral blood mononuclear cells (PBMC)
  • Primary lymphocyte sub-populations
  • Lymphocyte cell lines
 Use and Major Equipment Required

For Research Use Only.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well culture plates
  • Non-sterile, 96- or 384-well plates
  • Instruction manual.
 ImmunoGlo™-Tox HT Quick Guide and Assay Manual

Download the ImmunoGlo™-Tox HT Quick Guide

The ImmunoGlo™-Tox HT Assay Manual can be accessed in the Resource section here