Products - ImmunoGlo
ImmunoGlo™-96
ImmunoGlo™-MLC
ImmunoGlo™-384 HT
ImmunoGlo™-96
ATP Bioluminescence Assays for Immune Cells
Available as assay kits or
contract research services
ImmunoGlo™-96 Applications
- Lymphocyte activation.
- Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
- Cellular immune response studies.
- Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
- Effect of accessory (non T-cells) on T cell induction.
- Effect of co-stimulators on T-cell induction.
- Effect of epitope sequences and novel peptides or proteins.
- Test the response of primed T-cells in vitro.
- In vitro immunotoxicity studies. See also ImmunoGlo™-384 HT)
Advantages of using ImmunoGlo™96
- For all assays involving lymphocyte proliferation.
- Non-radioactive assay incorporating proven Bioluminomics™ Technology.
- Instrument-based, quantitative and fully standardized allowing comparison of results over time.
- 10 - 100 times more sensitive than WST-1, CSFE an other absorbance or fluorescence assays.
- Available in 96- or 384-well plate formats for high throughput toxicity studies. (See ImmunoGlo™-384 HT).
- Complete flexibility to use any mitogens, co-stimulators etc and lymphocyte culture protocols.
- Multiplexing capability to incorporate flow cytometry, cytokine release and other assay readouts for additional information from the same sample.
Species
ImmunoGlo™-96 can be used to study lymphocytes from many different species, including, but not limited to:
- Human
- Non-human primate
- Dog
- Rat
- Mouse
Tissues
- Peripheral blood mononuclear cells (PBMC).
- Lymphocyte sub-populations.
- Lymphocyte tissues and organs
Use
For Research Use Only. Not for clinical diagnostic use.
Major Equipment Required
Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.
Kit Contents
- ATP standards
- ATP high and low controls
- ATP Enumeration Reagent
- Sterile, 96-well culture plates
- Non-sterile, 96-well plates for ATP standard curve
- Sterile, adhesive plate cover foils to maintain sterility of unused wells
- Instruction manual
Related Products
ImmunoGlo™-MLC
ATP Bioluminescence Mixed Lymphocyte Culture (MLC) / Reaction (MLR) Assays
Available as assay kits or
contract research services
ImmunoGlo™-MLC Applications
- One-way mixed lymphocyte culture (MLC) / reaction (MLR).
- Two-way mixed lymphocyte culture (MLC) / reaction (MLR).
- Allogeneic mixed lymphocyte culture.
- Cellular immune response.
- Dendritic cell interactions.
- Mesenchymal stem/stromal cell interactions.
- Agents that activate/suppress MLC/MLR.
Advantages of using ImmunoGlo™-MLC
- Non-radioactive assay incorporating proven Bioluminomics™ Technology. Can replace all radioactive MLC/MLR assays.
- Instrument-based, quantitative and fully standardized readout procedure.
- 10 - 100 times more sensitive than WST-1, CSFE an other absorbance or fluorescence assays.
- Complete flexibility to use virtually any MLC/MLR protocol.
- Reliably compare results over time.
- Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
Species
- Applicable to multiple species.
Tissues
- Lymphocytes and lymphocyte sub-populations from a variety of sources.
- Lymphocyte cell lines.
- Other potential immunoreactive cell types.
Use
For Research Use Only. Not for clinical diagnostic use.
Major Equipment Required
Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.
Kit Contents
- Mitomycin-C for one-way MLC/MLR
- ATP standard
- ATP high and low controls
- ATP Enumeration Reagent
- Sterile, 96-well culture plate(s)
- Non-sterile, 96-well plate(s)
- Sterile adhesive plate cover foils to maintain sterility of unused wells
- Instruction manual.
Related Products
ImmunoGlo™-HT
ATP Bioluminescence High-Throughput Immunotoxicity / Lymphocyte Proliferation Screening Assays
Available as assay kits or
contract research services
ImmunoGlo™-Tox HT Applications
- In vitro high-throughput immunotoxicity screening
- In vitro high throughput lymphocyte proliferation screening
- Cellular immune response screening
Advantages of using ImmunoGlo™-Tox HT
- Non-radioactive assay incorporating proven high-throughput Bioluminomics™ Technology.
- Instrument-based, quantitative and fully standardized allowing comparison of results over time.
- Can be validated for GLP studies.
- More sensitive than WST-1, CSFE an other absorbance or fluorescence assays.
- Available in 96- or 384-well plate formats.
- Multiplexing capability to incorporate flow cytometry, cytokine release and other assay readouts for additional information from the same sample.
Species
- ImmunoGlo™-Tox HT can be used for many different species.
Tissues
- Peripheral blood mononuclear cells (PBMC)
- Primary lymphocyte sub-populations
- Lymphocyte cell lines
Use
For Research Use Only.
Major Equipment Required
Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.
Kit Contents
- ATP standard
- ATP high and low controls
- ATP Enumeration Reagent
- Sterile, 96- or 384-well culture plates
- Non-sterile, 96- or 384-well plates
- Instruction manual.
Related Assays
- HALO®-Tox HT
- HALO®-96 PRT
- LUMENESC™-Tox HT
- LumiSTEM™-Tox HT
- LumiSTEM™-96 PRT
- LumiCYTE™-HT
- LumiSTEM™-NeuroSC
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20% of all ImmunoGlo™ Assay Kits
Summary List of Assays
Contract Research Services
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