ImmunoLIGHT™-96

 

Colorimetric/Absorbance Assays for Immune Cells

 

Buy ImmunoLIGHT™-96 Products

Available as assay kits or
contract research services

 

ImmunoLIGHT™-96 Applications

• Lymphocyte activation.
• Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
• Cellular immune response studies.
• Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
• Effect of accessory (non T-cells) on T cell induction.
• Effect of co-stimulators on T-cell induction.
• Effect of epitope sequences and novel peptides or proteins.
• Test the response of primed T-cells in vitro.

 

Advantages of using ImmunoLIGHT™-96

• Uses a colorimetric/absorbance ELISA plate reader, available in most laboratories.
• Non-subjective, instrument-based and quantitative.
• Flexibility to use virtually any protocol, reagents and growth factors/cytokines or other immune agent stimulators or suppressors.
• Assay kit includes supplies for culture and cell processing.
• Easy to learn, fast to use.

 

Species

ImmunoLIGHT-96 can be used to study lymphocytes from many species including, but not limited to:

• Human
• Non-human primate
• Mouse

 

Tissues

• Peripheral blood mononuclear cells (PBMC)
• Lymphocyte sub-populations
• Lymphocyte tissues and organs

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Absorbance plate reader or multiparameter plate reader with an absorbance filter at 490nm.

 

Kit Contents

• MTS Reagent
• Sterile, clear 96-well plates
• Sterile, adhesive foil covers
• Instruction manual

 

Related Products

• ImmunoGlo™-96
• ImmunoGlo™-MLC
• ImmunoFLUOR™-96
• HALO®-96 Research (for stem cell and lymphocyte progenitor cells)
• CAMEO™-4 (for stem cell and lymphocyte progenitor cells)
• CAMEO™-96 (for stem cell and lymphocyte progenitor cells)

ImmunoLIGHT™-MLC

Absorbance Mixed Lymphocyte Culture (MLC) / Reaction (MLR) Assays

 

Buy ImmunoLIGHT™-MLC Products

Available as assay kits or
contract research services

 

ImmunoLIGHT™-MLC Applications

• One-way mixed lymphocyte culture (MLC) / reaction (MLR).
• Two-way mixed lymphocyte culture (MLC) / reaction (MLR).
• Allogeneic mixed lymphocyte culture.
• Cellular immune response.
• Dendritic cell interactions.
• Mesenchymal stem/stromal cell interactions.
• Agents that activate/suppress MLC/MLR.

 

Advantages to using ImmunoLIGHT™-MLC

• Uses a colorimetric/absorbance ELISA plate reader, available in most laboratories.
• Non-subjective, instrument-based and quantitative.
• Flexibility to use virtually any protocol, reagents and growth factors/cytokines or other immune agent stimulators or suppressors.
• Assay kit includes supplies for culture and cell processing.
• Easy to learn, fast to use.

 

Species

• Applicable to multiple species

 

Tissues

Lymphocytes and lymphocyte sub-populations from a variety of sources.
Lymphocyte cell lines.
Other potential immunoreactive cell types.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Absorbance plate reader or multiparameter plate reader with a 490nm absorbance filter.

 

Kit Contents

• Mitomycin-C for one-way MLC/MLR
• MTS Reagent
• Sterile, clear 96-well plates
• Sterile, adhesive foil covers
• Instruction manual

Related Assays

• ImmunoFLUOR™-MLC
• ImmunoGlo™-MLC
• ImmunoGlo™-96
• ImmunoFLUOR™-96
• ImmunoLIGHT™-96