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Products - ImmunoLIGHT™
 
  •  ImmunoLight™: Lymphocyte

    Proliferation Assays

  • ImmunoLight™ MLC: Mixed

    Lymphocyte Culture


ImmunoLight™

ImmunoLight™ TCP

 

Fast, Reliable, 96-Well Absorbance Complete Assay Kit for Immune Cell Functionality and Lymphocyte Proliferation

 

Buy ImmunoLIGHT™ Assay Kits

 

Buy ImmunoLIGHT™ TCP Assay Kits

 

 
Uses of ImmunoLight™ and ImmunoLight™ TCP
  • Lymphocyte activation.
  • T-lymphocyte proliferation (ImmunoLight™-96 TCP)
  • Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
  • Cellular immune response studies.
  • Single cell, T-lymphocyte cloning studies.
  • Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use.
  • Effect of accessory (non T-cells) on T cell induction.
  • Effect of co-stimulators on T-cell induction.
  • Effect of epitope sequences and novel peptides or proteins.
  • Test the response of primed T-cells in vitro.

 

Benefits of using ImmunoLight™-96 and ImmunoLight™-96 TCP
  • Uses a colorimetric/absorbance ELISA plate reader, available in most laboratories.
  • Non-subjective, instrument-based and quantitative.
  • Flexibility to use virtually any protocol, reagents and growth factors/cytokines or other immune agent stimulators or suppressors.
  • Assay kit includes supplies for culture and cell processing.
  • Easy to learn, fast to use.

 

 Species

ImmunoLIGHT-96 can be used to study lymphocytes from many species including, but not limited to:

  • Human
  • Non-human primate
  • Mouse
 Tissues
  • Peripheral blood mononuclear cells (PBMC)
  • Lymphocyte sub-populations
  • Lymphocyte tissues and organs
 ImmunoLight™-96 and ImmunoLight™-96 TCP Assays Available

ImmunoLight™-96 Assays Available

Assay Type Catalog Number Number of Plates/Kit

Number of Samples/Kit(#)

ImmunoLight™-96

KM3-LPA-1

1

16 - 24

ImmunoLight™-96

KM3-LPA-2

2

32 - 48

ImmunoLight™-96

KM3-LPA-4

4

64 - 96

 (#): HemoGenix® recommends a minimum of 6 replicates/sample. However, this can be reduced to 4 replicates/sample

 

ImmunoLight™-96 TCP

T-Cell Proliferation Assays for Human Cells

Assay Type Catalog Number HemoGro™ Serum-Free containing:

Number of Plates/Samples per Kit

ImmunoLight™-96 TCP

KM3-1-1H

IL-2 alone

1 / 16-24

ImmunoLight™-96 TCP

KM3-TCS1-1H

CD3 + CD28

1 / 16-24

ImmunoLight™-96 TCP

KM3-TCS2-1H

IL-2 + CD3 + CD28

1 / 16-24

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Absorbance plate reader or multiparameter plate reader with an absorbance filter at 490nm.

 Assay Kit Contents
ImmunoLight™ Assay Kits
  • MTS Reagent
  • Sterile, clear 96-well plates
  • Sterile, adhesive foil covers
  • Assay manual

 

ImmunoLight™-96 TCP Assay Kits
  • T-cell Culture and Expansion Master Mix in Serum-Free, ImmunoGro™ Medium
  • MTS Reagent
  • Sterile, clear 96-well plates
  • Sterile, adhesive foil cover to maintain sterility for unused wells
  • Assay manual
 ImmunoLight™-96 and TCP Information FlyerQuick Guide and Assay Manual

ImmunoLight™-96 Information Flyer

ImmunoLight™-96 TCP Information Flyer

ImmunoLight™-96 & TCP QuickGuide

ImmunoLight™-96 @ TCP Assay Manual. Also available on the Resource Page

ImmunoLight™ MLC

Fast and Reliable, 96-Well Absorbance, 1- or 2-Way Mixed Lymphocyte Culture (MLC) / Reaction (MLR) Assay Kit

 

Buy ImmunoLIGHT™ MLC Assay Kits

 

 

 

Uses of ImmunoLight™ MLC
  • One-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Two-way mixed lymphocyte culture (MLC) / reaction (MLR).
  • Allogeneic mixed lymphocyte culture.
  • Cellular immune response.
  • Dendritic cell interactions.
  • Mesenchymal stem/stromal cell interactions.
  • Agents that activate/suppress MLC/MLR.

 

Benefits of using ImmunoLight™ MLC
  • Uses a colorimetric/absorbance ELISA plate reader, available in most laboratories.
  • Non-subjective, instrument-based and quantitative.
  • Flexibility to use virtually any protocol, reagents and growth factors/cytokines or other immune agent stimulators or suppressors.
  • Assay kit includes supplies for culture and cell processing.
  • Easy to learn, fast to use.

 

 Species
  • Applicable to multiple species
 Tissues

Lymphocytes and lymphocyte sub-populations from a variety of sources.
Lymphocyte cell lines.
Other potential immunoreactive cell types.

 ImmunoLight™-MLC Assays Available

ImmunoLight™-MLC Assays Available

Assay Type Catalog Number Number of Plates/Kit

Number of Samples/Kit(#)

Click to see price

ImmunoLight™-MLC

KM3-MLC-1

1

16 - 24

Price
ImmunoLight™-MLC

KM3-MLC-2

2

32 - 48

Price
ImmunoLight™-MLC

KM3-MLC-4

4

64 - 96

Price

 (#): HemoGenix® recommends a minimum of 6 replicates/sample. However, this can be reduced to 4 replicates/sample

 Use and Major Equipment Requirement

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Absorbance plate reader or multiparameter plate reader with a 490nm absorbance filter.

 Assay Kit Contents
  • Mitomycin-C for one-way MLC/MLR
  • MTS Reagent
  • Sterile, clear 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual
 ImmunoLight™-MLC Quick Guide and Assay Manual

Download the ImmunoLight™-Quick Guide

The ImmunoLight™-MLC Assay Manual can be found in the Resources section here