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Products - LUMENESC

  • LUMENESC™-96
    Research

  • LUMENESC™-96
    HuQC

  • LUMENESC™-96
    PQR 

  • LUMENESC™
    Tox HT

  •  LUMENESC™-384 DDI

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LUMENESC™-96 Research


Assays for the Mesenchymal Stem/Stromal Cell System

 

Buy LUMENESC™-96 assay kits

These assays are available as assay kits or as contract research services.

 


LUMENESC™-96 Applications
  • All research applications involving mesenchymal stem/stromal cells (MSC) and cells derived from MSCs.
  • A replacement for the colony-forming unit - fibroblast (CFU-F) assay.
  • Stromal cell assay applications.
  • Self-renewal and differentiation capability applications.
  • Clinical and regenerative medicine applications (see also LUMENESC™-96 HuQC and LUMENESC™-96 PQR)
  • Determine the effects of growth factors/cytokines on MSCs and their differentiation.
  • MSC expansion.
  • In vivo to in vitro assays.
  • MSC gene targeting assays.

 

Advantages of using LUMENESC™-96
  • LUMENESC™-96 is an instrument-based, quantitative assay to measure MSC proliferation ability, potential and differentiation.
  • Provides more reliable and robust information than the subjective, manual CFU-F assay.
  • Choose between low serum and serum-free media assays or humanized media for human cells.
  • Monitor small, medium and large (bulk) MSC expansion (passaging).
  • Includes MSCGro™ Medium which demonstrates far superior growth and stability that most competitor media.
  • All assays include standards and controls to calibrate and standardize and, if necessary, validate the assay.
  • Multiplex with other assays such as flow cytometry for MSC and differentiation markers and gene expression analysis to obtain and correlate information from a single sample.
  • Incorporates proven bioluminomics™ technology that is fast to learn, easy to use and provides reliable and reproducible results.

 

Species
  • Human
  • Non-human primate
  • Rat
  • Mouse

 

MSCGro™ Media Available
  • Low serum
  • Serum-free
  • Humanized (for human cells only)

 

MSCGro™ Differentiation Media Available
  • Chondrocyte differentiation
  • Adipocyte differentiation
  • Osteoblast differentiation

 

Mesenchymal Stem/Stromal Cells Available
  • Umbilical cord blood-derived MSCs
  • Green fluorescent-labeled MSCs
  • Red fluorescent-labveled MSCs
  • GFP-labeled MSCs

 
Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents
  • MSCGro™ Medium and/or MSCGro™ Differentiation Medium
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

 

LUMENESC™-96 Assay Kit Manual

To download the assay kit manual, please go to the Downloads Page 

 

Related Assays

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LUMENESC™-96 HuQC

Assays for Mesenchymal Stem/Stromal Cell Quality Control in the Clinical or Regenerative Medicine Setting

 

Buy LUMENESC™-96 HuQC assay kits

These assays available as kits or
as contract research services.

 


LUMENESC™-96 HuQC Applications
  • For quantifying the "quality" of mesenchymal stem/stromal cells in a stem cell product prior to use.
  • To determine whether a processing procedure affects mesenchymal stem/stromal cell "quality".
  • To determine mesenchymal stem/stromal cell "quality" pre- and post cryopreservation.
  • To determine mesenchymal stem/stromal cell processing consistency and stability.

 

Advantages of using LUMENESC™-96 HuQC in a Clinical or Regenerative Medicine Setting
  • LUMENESC™-96 HuQC quantitatively measures proliferation ability and cellular functionality that cannot be enumerated using total nucleated counts (TNC), viability or flow cytometric detection of membrane expression markers.
  • LUMENESC™-96 HuQC determines "quality", that is the ability of mesenchymal stem/stromal cells to proliferate.
  • LUMENESC™-96 HuQC includes MSCGro™ Medium, which demonstrates far superior growth and stability properties than virtually any other MSC-specific media. 
  • Instrument-based, incorporating an external standard and controls for assay standardization and validation according to FDA regulations.
  • Compare results over time for intra- and inter-laboratory studies.
  • Assay can usually be completed in 5-7 days of culture depending on the batch of MSCs.
  • Faster, easier to use and far more accurate than the traditional CFU-F assay.
  • LUMENESC™-96 HuQC incorporates Bioluminomics™ Technology, the most advanced and cost-effective MSC in vitro assay system available.

 

MSCGro™ Media Available for Human MSC Culture
  • Low serum
  • Serum-free
  • Humanized

Comprable Assays - More Information

The counterpart to LUMENESC™-96 HuQC is HALO®-96 SPC-QC for lympho-hemopoietic stem cells. For more information on how stem cell "quality" is measured, please navigate to HALO®-96 SPC-QC, Background: Cellular Therapeutics: Stem Cell Quality Control or Applications: Cellular Therapeutics. 

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability. 

 
Kit Contents
  • MSCGro™ Medium of choice
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

 

LUMENESC™-96 HuQC Assay Kit Manual

To download the assay kit manual, please go to the Downloads Page.

 

Related Assays

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LUMENESC™-96 PQR

A Reference Standard-Based Potency, Quality and Release Assay for Mesenchymal Stem/Stromal Cells

 

Buy LUMENESC™-96 PQR assay kits

These assays available as assay kits or as contract research services.

 

LUMENESC™-96 PQR Applications
  • To determine mesenchymal stem/stromal cell potency and "quality" and help define acceptance limits for release criteria prior to use in clinical or regenerative medicine applications.

 

Advantages of using LUMENESC™-96 PQR
  • LUMENESC™-96 PQR is the only reference standard-based potency assay for mesenchymal stem/stromal cells commercially available.
  • LUMENESC™-96 PQR is based on the same proven principles of measuring potency of lympho-hematopoietic stem cells as HALO®-96 PQR.
  • Includes additional standards and controls which, together with the reference standard, allows the assay to be validated.
  • Includes MSCGro™ Medium which demonstrates superior growth and stability properties than most commercially available mesenchymal stem cell media. 
  • LUMENESC™-96 PQR is a potency assay that is compliant with FDA regulations for potency assays.
  • Incorporates proven bioluminomics™ technology for easy to use, fast and reliable results.

 

MSCGro™ Media Available
  • Low serum
  • Serum-free
  • Humanized

Comparable Assays - More Information

The counterpart to LUMENESC™-96 PQR is HALO®-96 PQR for lympho-hematopoietic stem cell potency. LUMENESC™-96 PQR uses exactly the same concepts and principles as HALO®-96 PQR. These as well as examples of how stem cell potency is measured can be found by navigating to HALO®-96 PQR and Backgound: Cellular Therapeutics: Stem Cell Potency.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents
  • MSCGro™ Medium of choice
  • Cryopreserved MSC reference standard
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

 

Related Assays

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LUMENESC™-Tox HT (LUMENESC™-96 Tox and LUMENESC™-384 HT)

A Toxicity Screening and Testing Platform for Cells of the Mesenchymal Stem/Stromal Cell System

 

Buy LUMENESC™-96 Tox assay kits

 

Buy LUMENESC™-384 HT assay kits

Assays are available as assay kits for in-house implementation and contract research services.

 

LUMENESC™-Tox HT Applications

  • Predictive in vitro to in vivo surrogate assay for human and animal MSC toxicity.
  • Predictive in vitro MSC screening and testing platform for all stages of drug development. 
  • Predictive in vitro MSC toxicity screening and testing for xenobiotic agents.
  • Incorporation into routine high throughput ADME/Tox screening.
  • Drug/ compound ranking according to species and/or cell population sensitivity.

 

Advantages of using LUMENESC™-Tox HT for Mesenchymal Stem/Stromal Cell Toxicity Testing
  • A 3Rs Alternative Assay Platform for Reduction, Refinement and Replacement of animal testing.
  • The only in vitro high throughput MSC toxicity assay platform available with both 96-well and 384-well plate formats (LUMENESC™-96 Tox and LUMENESC™-384 HT).
  • Rapid turnaround: results in about 7 days days.
  • Uses MSCGro™ Media, shown to be far superior in growth and stability properties than most MSC-specific media.
  • Incorporates proven Bioluminomics™ Technology for the most advanced and reliable MSC toxicity assays available.
  • Designed for multiplexing with other assays to produce the most amount of information from a single sample.

 

Species
  • Human
  • Non-human primate (Cynomologus and Rhesus)
  • Rat
  • Mouse

 

Tissues

LUMENESC™-Tox HT can be used with MSCs derived from fresh primary tissues such as bone marrow and umbilical cord blood or MSCs derived from other cells such as induced pluripotent stem cells (iPSC).

 

MSCGro™ Media available

  • Low serum
  • Serum-free
  • Humanized (only for human MSCs)

Comparable Assays - More Information

The counterpart assays to LUMENESC™-Tox HT are HALO®-Tox HT and LumiSTEM™-Tox HT  and other information is also present at Background: InVitro Toxicology. Please navigate to these areas of the website to see more information.

 

Use

For Research Use Only.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents
  • LUMENESC™-Tox HT containing MSCGro™
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers
  • Instruction manual

Related Assays

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LUMENESC™-384 DDI

A Cell-Based Drug-Drug Interaction Assay Platform for Mesenchymal Stem/Stromal Cells

 

Only available as a
contract research service.

 


LUMENESC™-384 DDI Applications
  • To determine the cellular effects of two or more drugs interacting with each other that might result in extreme changes to mesenchymal stem/stromal cells and their differentiated progeny.

 

Advantages of using LUMENESC™-384 DDI to Investigate Drug-Drug Interactions
  • LUMENESC™-384 DDI can detect potentially harmful drug-drug interactions to the mesenchymal stem/stromal cell system. Drug-drug interactions are usually detected by induction or inhibition of cytochrome P450 enzymes in hepatocytes of the liver. However, these enzymes are also present in mitochondria of cells of other tissues and organs and can result in potentially serious local perturbations. 
  • LUMENESC™-384 DDI is the only cell-based in vitro drug interaction platform available for cells of the mesenchymal stem cell system.
  • LUMENESC™-384 DDI can be combined with HALO®-384 DDI and LumiSTEM™-384 DDI to compare drug interactions on multiple cell types and species.
  • LUMENESC™-384 DDI uses proven Bioluminomics™ Technology to provide an advanced, powerful and reliable assay platform for toxicity testing.

 

Species
  • Human
  • Non-human primate
  • Rat
  • Mouse

Hemogenix Inc.