LumiSTEM™-Tox HT (LumiSTEM™-96 Tox and LumiSTEM™-384 HT)

A Toxicity Screening and Testing Platform for Multiple Cell Types from Different Cell Systems and Species

 

Buy LumiSTEM™-96 Tox assay kits

 

Buy LumiSTEM™-384 HT assay kits

Assays are available as assay kits for in-house implementation and contract research services.

 

LumiSTEM™-Tox HT Applications

• Toxicity screening and testing assay for any stage of drug development.
• Toxicity screening and testing for xenobiotic agents.
• Incorporation into routine high throughput ADME/Tox screening.
• Drug / compound ranking according to organ / tissue, cell system, cell type and species.

 

Advantages of using LumiSTEM™-Tox HT for Toxicity Testing

• A 3Rs Alternative Assay Platform for Reduction, Refinement and Replacement of animal testing.
• An in vitro high throughput assay platform available with both 96-well and 384-well plate formats (LumiSTEM™-96 Tox and LumiSTEM™-384 HT).
• Rapid turnaround: from hours to days depending on cell type tested.
• Complete flexibility to use protocols, reagents and culture conditions.
• Standards and controls allow assay calibration and standardization providing the ability to directly compare cell toxicities from different organs/ tissues and species (see also ComparaTOX™) 
• Incorporates proven Bioluminomics™ Technology for the most advanced and reliable toxicity assays available.
• Designed for multiplexing with other assays to produce the most amount of information from a single sample.

 

Species

• Multiple

 

Cell Types that can be used with LumiSTEM™-96 assays

Stem Cell Systems:

• Primary stem cells from different tissues and organs
• Cancer stem cells
• Induced pluripotent stem cells (iPS, see LumiSTEM™-iPS HT)
• Embryonic stem (ES) cells

 

Cell Systems:

• Fibroblastoid
• Epithelial
• Endothelial
• Muscle
• Skeletal

 

Primary Explanted Cells from:

 

Specific Cell Types that can be used with LumiSTEM™-96:

 

Cell lines

• Virtually any cell line 

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents

• ATP standard
• ATP high and low controls
• ATP Enumeration Reagent
• Sterile, 96-well plates
• Non-sterile, 96-well plates
• Sterile, adhesive foil covers
• Instruction manual

LumiSTEM™-96 PRT

A Predictive Residual Toxicity Assay for Stem Cells

 

Buy LumiSTEM™- 96 PRT assay kits

Available as assay kits and

contract research services.

 

LumiSTEM™-96 PRT Applications

• To determine the proportion of stem cells remaining or resistant to drug administration.
• To determine whether stem cells change their sensitivity to a drug after a single dose.
• To determine the effect on primitive stem cells to multiple drug administration.
• To determine whether stem cells exhibit expansion potential after a single drug dose.

 

Advantages of using LumiSTEM™-96 PRT to Detect Residual Stem Cell Toxicity

• Can reduce animal testing (3Rs) and be applied directly to fresh, primary human stem cells.
• The only in vitro assay available that can detect whether unaffected stem cells remain after drug administration.
• The only assay available that indicates changes in drug sensitivity to stem cells.
• Uses a 2-stage stem cell "priming" and cell expansion in vitro re-plating technology.
• Incorporates proven bioluminomics™ technology.
• Designed for multiplexing with other assays, including flow cytometry and gene expression analysis, to obtain the most information from a single sample.

 

Species

• Multiple

 

Stem Cell Populations

• Multiple (e.g. primary stem cell, ES and iPS cells, cancer stem cells)

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents

• ATP standard
• ATP high and low controls
• ATP Enumeration Reagent
• 2 or 4 sterile, 96-well plates
• Non-sterile, 96-well plates
• Sterile, adhesive foil covers
• Instruction manual

LumiCYTE™-HT

A Bioluminomics™ Assay Platform Designed for Hepatotoxicity

 

This assay platform is only available as contract research services.

 

For assays that can also be used for hepatocytes please refer to LumiSTEM™-96 or LumiSTEM™-Tox HT.

 

LumiCYTE™-HT Applications

• Specifically designed to screen and test for hepatotoxicity.

 

Advantages of using LumiCYTE™-HT

• Fully calibrated and standardized assay for viability, cell functionality and cell number allowing results to be compared over time.
• Incorporates proven bioluminomics™ technology, the most sensitive, non-radioactive signal detection system available.
• Designed for multiplexing with other assays to produce the most amount of information from a single sample.

 

Species

• Human
• Non-human primate
• Dog
• Rat
• Mouse
• Please inquire for other species

 

Hepatocytes

• Fresh
• Cryopreserved
• Male
• Female
• Pooled
• Hepatocyte cell lines 

 

Examples using LumiCYTE™-HT

The two diagrams to the left compare the response of 5-fluorouracil and doxorubicin on cryopreserved human and rat hepatocytes. Notice the different scale on the Y-axis for the two species. Although hepatocytes do not represent a proliferating cell population under normal steady-state conditions, they are actively producing intracellular ATP. The difference in normal ATP production is shown on the Y-axis. For the examples shown here, LumiCYTE™-HT provides an reliable high throughput assay platform to detect hepatotoxicity during ADME/Tox screening. Furthermore, the LumiSTEM™-384 DDI is a perfect addition to the hepatotoxicity screening platform for drug-drug interaction. Please contact HemoGenix® for more information. 

 

 

 

 

 

LumiSTEM™-384 DDI

A Cell-Based Drug-Drug Interaction Assay Platform

 

 

LumiSTEM™-384 DDI is only available as a contract research services.

 

 

LumiSTEM™-384 DDI Applications

• To determine the cellular effects of two or more drugs interacting with each other.

 

Why Use LumiSTEM™-384 DDI to Investigate Drug-Drug Interactions?

• LumiSTEM™-384 DDI can detect potentially harmful drug-drug interactions to different cell types and cell systems from multiple species. Drug-drug interactions are usually detected by induction or inhibition of cytochrome P450 enzymes. However, these enzymes are also present in the mitochondria of cells from other tissues and organs and can result in potentially serious local perturbations. 
• LumiSTEM™-384 DDI is the only cell-based in vitro drug interaction platform.
• LumiSTEM™-384 DDI can be combined with LUMENESC™-384 DDI and HALO®-384 DDI to compare drug interactions on multiple cell types and species (see also ComparaTOX™).
• LumiSTEM-384 DDI can be combined with LumiCYTE™-HT to provide drug-drug interaction information at the cellular hepatocyte level. 
• LumiSTEM™-384 DDI uses proven bioluminomics™ technology to provide an advanced, powerful and reliable assay platform for toxicity testing.

 

Species

• Human
• Non-human primate
• Dog
• Rat
• Mouse
• Depending on cell type tested, not all species may be available for testing.

 

Tissues

• Multiple