Cell Culture Growth and Differentiation Media

 

Buy MSCGrow Media

 

MSCGro™ Media for Mesenchymal Stem Cell (MSC) Growth

MSCGro™ Media are incorporated into all LUMENESC™ assay kits for MSC. 

 

Types of MSCGro™ Media

• Low serum
• Serum-free
• Humanized
• Humanized, serum-free

 

Species

• Human
• Non-human primate
• Rat
• Mouse

 

Quantities available

• 100ml
• 500ml
• 10L Humanized, clinical grade, powder formulation
• 10L Serum-free, powder formulation

 

MSCGro™ Media Information

MSCGro™ Low Serum or Serum-Free Complete Media

These media does not require any addition supplements. They support human, primate, rat and mouse MSC growth and continuous self-renewal.

MSCGro Serum-free media does not require prior treatment of culture flasks, plates or dishes to achieve full attachment.

MSCGro™ Humanized Complete Media

This media does not require any additional supplements and is for growth of human MSCs under continuous self-renewal.

MSCGro™ Humanized Clinical Grade Media

This is similar to MSCGro Humanized Complete Media, but without human serum. This medium is intended for clinical studies using autologous, humanized, and zeno-free medium for ex-vivo expansion and/or differentiation of patient-specific MSCs from bone marrow, adipose tissue and cord blood. It requires the addition of autologous human serum prior to use or alternatively, an allogeneic serum that may be purchased separately.

MSCGro™ Humanized Clinical Grade, Powder Formulation Medium

This media is provided as a powder with a liquid supplement. The powdered medium is reconstituted with USP-WFI (USP water for injection) prior to the addition of the concentrated supplement supplied with the powder. This medium is provided for a 10L batch. Directions for reconstitution and preparation are provided with each shipment.

MSCGro™ Serum-Free Complete Powder Formation Medium

This is a dried base medium with a liquid supplement. The medium must be reconstituted with USP-WFI water prior to the addition of the supplement. Instructions for preparation are included. This medium is supplied for 10L.

 

Competitive Advantages of MSCGro™ Media

• MSC growth rate/cell recovery is greater than using media from Lonza, Invitrogen, Stem Cell Technologies, ScienCell and ZenBio. (See below).
• MSC quality and potency measured using LUMENESC™-96 PQR is greater than media from Lonza, Invitrogen and Stem Cell Technologies. (See below).
• Competitors media cannot compare with the real time stability of MSCGro™, which shows a stability of more than 1 year. (See below).
• MSCGro™ Media is unaffected by freezing.
• MSCGro™ Media outperforms competitors media at both reduced (5%) and ambient oxygen tensions.
• MSCGro™ media supports expansion and self-renewal of human, primate, rat and mouse MSCs.
• MSCGro™ Differentiation Medium reliably induces production of chondrocytes, adipocytes and osteoblasts. 

Comparison of MSCGro™ Media with Other Competitor Brands

The growth of mesenchymal stem cells (MSC) has been compared to other MSC growth media by measuring MSC doubling time and using LUMENESC™-96, the bioluminomics™ replacement assay for the manual CFU-F assay and stability of the media. The results demonstrate that MSCGro™ MSC Media outperforms the competitors media when MSC are grown either under normal oxygen tension conditions or under low (5%) oxygen tension conditions. In addition, MSCGro™ exhibits the greatest stability and shelf-life of all the media tested.

LUMENESC™ Assay Kits with MSCGro™ Media

LUMENESC™-96 Research Assay Kits

LUMENESC™-96 HuQC, human MSC quality control assay kits

LUMENESC™-96 PQR, human MSC Potency, Quality, Release assay kits

LUMENESC™-96 Tox, MSC toxicity assay kits

LUMENESC™-384 HT, MSC high throughput toxicity assay kits

MSCGro™ Differentiation Media for Mesenchymal Stem Cell (MSC) Differentiation

Types of Media for MSC Differentiation

• Osteogenic differentiation
• Adipogenic differentiation
• Chondrogenic differentiation

 

Type of MSCGro™ Differentiation Media

• Low serum. Can be used for human, rat and mouse differentiation.
• Serum-free
• Humanized, containing human serum

 

Species

• Human

  

Quantities available

• 100ml
• 500ml

 Cells - Fresh and Cryopreserved

 

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Mesenchymal Stem Cells (MSC)

Species

• Human

 

Cells derived from:

• Umbilical cord blood

 

MSC Types

Fresh and Cryopreserved Mesenchymal Stem Cells

Description	Species	Size
Unlabeled MSC, native	Human	500,000 cryopreserved cells
Green fluorescent-labeled MSC	Human	500,000 cryopreserved cells
Red fluorescent-labeled MSC	Human	500,000 cryopreserved cells
GFP-labeled MSC	Human	500,000 cryopreserved cells

Accessories for Bioluminomics™ Assay Kits 

Buy Cell Culture Plates

Cell Culture Plates

Additional cell culture plates can be purchased. These include:

 

Additional Growth Factor Master Mixes for HALO®, CAMEO™-4 and
CAMEO™-96

Buy Growth Factor Master Mixes

(Please note that Master Mixes are only available to pre-existing customers)

Species

• Human
• Non-human primates
• Dog
• Rat
• Mouse

 

Cell Populations

 

ATP Standard Curve Reagents

Buy ATP Standard Curve Reagents

(Please note that these reagents are only available to pre-existing customers)

• ATP standard
• High and low controls

 

Plate Luminometer Optimization Kit

Buy Luminometer Optimization Kit

(Please note that these reagents are only available to pre-existing customers)

• This kit is for optimizing plate luminometer settings

 

Red Blood Cell Lysis Reagent

Buy Red Blood Cell Lysis Reagent

This reagent is used to lyse red blood cells in samples with high (>10%) erythrocyte concentrations that can interfere with bioluminomics™ assays.