MSCFluor™-96

 

Fluorescence Assays for Mesenchymal Stem / Stromal Cells

 

Buy MSCFluor™-96 Products

Available as assay kits or
contract research services

 

MSCFluor™-96 Applications

• Determine the number of viable mesenchymal stem / stromal cells in proliferation.
• Replaces the colony-forming unit - fibroblast (CFU-F) assay.
• Experimental transplantation and regeneration models.
• Effects of growth factors/cytokines.
• In vivo to in vitro assays.
• Gene targeting assays.

 

Advantages of using MSCFluor™-96

• No manual CFU-F colony counting needed.
• Non-subjective, instrument-based and quantitative.
• Includes high performance MSCGro™ Medium.
• Multiplexes with bioluminescence assays.
• Results in about 3-5 days depending on species and origin of MSC.
• Allows the cells to be detected and characterized by flow cytometry using other excitation and emission wavelengths.
• Easy to learn, fast to use.

 

Tissues

• Bone marrow
• Umbilical cord blood
• Induced pluripotential stem (iPS) cell-derived MSCs
• Other cells and tissues from which MSC can be derived

 
MSCGro™ Media

MSCGro media is available in the following formulations and included with every MSCLight-96 assay kit of choice:
Low serum
Serum-free
Humanized serum (only for human MSCs)

 

MSCFluor™-96 Principle

• Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
• After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time: 2 hours.
• Use a fluorescence plate reader or multiparameter plate reader capable of measuring fluorescence (380-400nm excitation/505nm emission).

 

Use

For Research Use Only.

 

Major Equipment Required

A fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.

 

Kit Contents

• MSCGro™ Medium of choice
• GF-AFC reagent
• Sterile, 96-well plate(s)
• Sterile, adhesive cover foils to maintain sterility of unused wells
• Instruction manual