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Products - MSCFluor
 
  •  MSCFluor™-96

MSCFluor-96 Research: Assays to measure mesenchymal stem/progenitor cell proliferation

MSCFluor™-96 Research

MSCFluor™-96 Complete

 

A Fast and Reliable, 96-Well, Fluorescence Assay Kit to Measure Mesenchymal Stem/Progenitor Cells (MSC/MPC) Proliferation and Functionality that

Replaces the CFU-F MesenCult™ Assay

 

Buy MSCFluor™-96 Research Assays

 

Buy MSCFluor™-96 Complete Assays

 

Applications / Research Areas
  • Mesenchymal stem / progenitor cells (MSC/MPC)
  • Immunoregulation
  • Regenerative medicine
  • Cell proliferation
  • Cell viability

 

Uses of MSCFluor™-96 Research
  • Determine the number of viable mesenchymal stem / stromal cells in proliferation.
  • Replaces the colony-forming unit - fibroblast (CFU-F) assay.
  • Experimental transplantation and regeneration models.
  • Effects of growth factors/cytokines.
  • In vivo to in vitro assays.
  • Gene targeting assays.

 

Benefits of using MSCFluor™-96 Research
  • No manual CFU-F colony counting needed.
  • Non-subjective, instrument-based and quantitative.
  • Includes high performance MSCGro™ Medium.
  • Multiplexes with bioluminescence assays.
  • Results in about 3-5 days depending on species and origin of MSC.
  • Allows the cells to be detected and characterized by flow cytometry using other excitation and emission wavelengths.
  • Available for multiple species, including dog and horse.
  • Easy to learn, fast to use.

Benefits of MSCFluor™-96 Complete

Same as for MSCFluor™-96 Research, but includes a vial of cryopreserved human MSCs derived from either umbilical cord blood or bone marrow.

 Species
  • Human
  • Non-human primate
  • Rat
  • Mouse
  • Dog (new)
  • Horse (new)

 MSC Growth Supplements and Media
  • CRUXRUFA Human Platelet Lysate
  • Low serum, complete
  • Serum-free, xenon-free complete
  • Humanized, complete
  • Canine, low serum complete
  • Canine, serum-free complete
  • Equine, low serum complete
  • Equine, serum-free complete

 MSCFluor™-96 Research Principle
  • Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
  • After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time: 2 hours.
  • Use a fluorescence plate reader or multiparameter plate reader capable of measuring fluorescence (380-400nm excitation/505nm emission).

 Use and Major Equipment Required

For Research Use Only.

Major Equipment Required

A fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.

 Assay Kit Contents
  • MSCGro™ Medium of choice
  • GF-AFC reagent
  • Sterile, 96-well plate(s)
  • Sterile, adhesive cover foils to maintain sterility of unused wells
  • Instruction manual
Kit Contents of MSCFluor™-96 Complete
  • 1 vial of cryopreserved human umbilical cord blood or bone marrow MSCs
  • MSCGro™ Medium of choice
  • GF-AFC reagent
  • Sterile, 96-well plate(s)
  • Sterile, adhesive cover foils to maintain sterility of unused wells
  • Instruction manual

 MSCFluor™-96 Research Information Flyer, QuickGuide and Assay Manual