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Products - MSCLight
 
  •  MSCLight™-96

MSCLight-96 Research: Assays to measure mesenchymal stem/progenitor cell proliferation

MSCLight™-96 Research

MSCLight™-96 Complete

A Fast and Reliable, 96-Well, Absorbance Assay Kit to Measure Mesenchymal Stem/Progenitor Cells (MSC/MPC) Proliferation and Functionality that
Replaces the CFU-F MesenCult™ Assay

 

Buy MSCLight™-96 Research Assays

 

Buy MSCLight™-96 Complete

 

Application / Research Areas
  • Mesenchymal stem / progenitor cells (MSC/MPC)
  • Immunoregulation
  • Regenerative medicine
  • Cell proliferation
  • Cell viability

 

Uses of MSCLIght™-96 Research
  • Determine the number of viable mesenchymal stem / stromal cells in proliferation.
  • Replaces the colony-forming unit - fibroblast (CFU-F) assay.
  • Replaces all MTT assays.
  • Experimental transplantation and regeneration models.
  • Effects of growth factors/cytokines.
  • In vivo to in vitro assays.
  • Gene targeting assays.

 

Benefits of using MSCLight™-96 Research
  • Uses an ELISA plate reader, available in most laboratories.
  • No CFU-F colony counting needed.
  • Non-subjective, instrument-based and quantitative.
  • Assay kits include high performance MSCGro™ medium with either low serum or serum free formulations.
  • MSCLight™-96 can be used with fluorescence-labeled and GLP-labeled mesenchymal stem cells from HemoGenix®.
  • Results in about 3-5 days depending on species and origin of MSC.
  • Complete assay kit includes everything needed to culture and measure mesenchymal stem cells from any tissue source.
  • Easy to learn, fast to use.
Benefits of using MSCLight™-96 Complete

Same as for MSCLight™-96 Research, but inclues a vial of frozen MSCs derived from human umbilical cord blood or bone marrow. 

 Species
  • Human
  • Non-human primate
  • Rat
  • Mouse
  • Dog (new)
  • Horse (new)

 MSC Growth Supplements and Media
  • CRUXRUFA Human Platelet Lysate
  • Low serum, complete
  • Serum-free, xenon-free complete
  • Humanized, complete
  • Canine, low serum complete
  • Canine, serum-free complete
  • Equine, low serum complete
  • Equine, serum-free complete

 MSCLight™-96 Research Principle
  • Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction colorimetric reaction.
  • After culture, just add 20μl of the MTS reagent and read absorbance at 490nm after 1-4 hours in a 96-well plate reader.
  • No solubilization step as in a MTT reaction.
  • Read results in an aborbance plate reader between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.

 Use and Major Equipment Required

For Research Use Only.

Major Equipment Required

An absorbance plate readr or multiparameter plate reader to measure absorbance at 490nm.

 Assay Kit Contents
  • MSCGro™ Medium formulation of choice
  • Sterile, 96-well plate(s)
  • Non-sterile, 96-well plate(s)
  • Sterile, adhesive cover foils to maintain sterility of unused wells
  • Instruction manual

Kit Contents for MSCLight™-96 Complete
  • 1 Vial of cryopreserved human umbilical cord blood or bone marrow MSCs
  • MSCGro™ Medium formulation of choice
  • Sterile, 96-well plate(s)
  • Non-sterile, 96-well plate(s)
  • Sterile, adhesive cover foils to maintain sterility of unused wells
  • Instruction manual

 MSCLight™-96 Research Quick Guide and Assay Manual

Download the MSCLight™-96 Research Quick Guide

The MSCLight™-96 Research Assay Manual can be accessed from the Resources section here