MSCLight™-96

Colorimetric / Absorbance Assays for Mesenchymal Stem / Stromal Cells

Available as assay kits and
contract research services

Determine the number of viable mesenchymal stem / stromal cells in proliferation.
Replaces the colony-forming unit - fibroblast (CFU-F) assay.
Replaces all MTT assays.
Experimental transplantation and regeneration models.
Effects of growth factors/cytokines.
In vivo to in vitro assays.
Gene targeting assays.

Uses an ELISA plate reader, available in most laboratories.
No CFU-F colony counting needed.
Non-subjective, instrument-based and quantitative.
Assay kits include high performance MSCGro™ medium with either low serum or serum free formulations.
MSCLight™-96 can be used with fluorescence-labeled and GLP-labeled mesenchymal stem cells from HemoGenix®.
Results in about 3-5 days depending on species and origin of MSC.
Complete assay kit includes everything needed to culture and measure mesenchymal stem cells from any tissue source.
Easy to learn, fast to use.

MSCGro media is available in the following formulations and included with every MSCLight-96 assay kit of choice:

Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction colorimetric reaction.
After culture, just add 20μl of the MTS reagent and read absorbance at 490nm after 1-4 hours in a 96-well plate reader.
No solubilization step as in a MTT reaction.
Read results in an aborbance plate reader between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.

For Research Use Only.

An absorbance plate readr or multiparameter plate reader to measure absorbance at 490nm.