XVPrime-Glo™ & XVPrime-Glo™ HT

An ATP Bioluminomics™ Assay Platform for Ex Vivo Primary Explanted Cells

Buy XVPrime-Glo assay kits

 

Buy XVPrime-HT assay kits Available as assay kits and
contract research services.

 

XVPrime™ Assay Applications

• Viability, cell functionality, proliferation / cytotoxicity and cell number assays for ex vivo primary explanted cells.
• Cell growth kinetic/expansion studies.
• Developmental biology.
• Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other reagents on primary stem cells and stem cell lines.
  Cell physiology, injury and disease studies.
• Cell-based therapies. 

 

Special XVPrime-Glo™ HT Applications

• High throughput in vitro toxicity screening and safety and efficacy testing.
• Can be incorporated at any stage of drug development, including ADME/Tox.
• Drug/agent toxicity ranking according to organ/tissue and species.

 

Advantages of using XVPrime-Glo™

• The most sensitive of any assay readouts incorporating proven ATP Bioluminomics™ Technology.
• Instrument-based, quantitative and fully standardized readout procedure.
• Available for adherent or non-adherent stem cells.
• Flexibility to use virtually any in vitro cell culture protocol, reagents and growth factors/cytokines.
• Can be used for cell quality control and potency testing.
• High throughput capability.
• Reliably compare results over time.
• Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
• Easy to learn, fast to use.

 

Special Advantages of using XVPrime-Glo™ HT

In addition to the advantages of using STEMGlo™, the high throughput in vitro toxicity platform has the following special advantages:

• A 3Rs alternative in vitro assay platform for Reduction, Refinement and Replacement of animal testing.
• 96- and 384-well plate availability.
• Rapid turnaround.
• Complete assay calibration, standardization and validation according to regulatory requirements.

 

Species

• Multiple

 

Cell Sources used with XVPrime-Glo™ or XVPrime-Glo™ HT

Ex Vivo Primary Explanted Cells from:

 

General and Specialized Cell Systems from Ex Vivo Primary Explanted Cell Sources:

• Fibroblastoid
• Epithelial
• Endothelial
• Muscle
• Skeletal
• Kertinocytes
• Melanocytes

 

Please note that for cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

Availability

XVPrime-Glo™ assays are available for:

• Research with 96-well plates
• In vitro toxicity screening and testing with 96- or 384-well plates

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents

• ATP standard
• ATP controls
• ATP Enumeration Reagent
• Sterile, 96- or 384-well plates, depending on assay use
• Non-sterile, 96- or 384-well plates, depending on assay use
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• XVPrime-Fluor™
• XVPrime-Light™
• XVPrime-Clone™
• HepatoGlo™-HT
• XVPrime™-PRT
• HALO®-96 Research: bioluminomics™ assays for human and animal lympho-hematopoietic stem cells
• HALO®-Tox HT: high throughput toxicity screening and testing of lympho-hematopoietic stem cells.
• HemoFLUOR™-96: fluorescence assays for lympho-hematopoietic stem cells
• HemoLIGHT™-96: absorbance assays for lympho-hematopoietic stem cells 
• LUMENESC™-96: bioluminomics™ assays for mesenchymal stem cells
• LUMENESC™-Tox HT: high throughput screening and testing of mesenchymal stem cells
• MSCFluor™-96: fluorescence assays for mesenchymal stem cells
• MSCLight™-96: absorbance assays for mesenchymal stem cells 

 

XVPrime-Fluor™

Ex Vivo Primary Explanted Cells
 

 

   Buy XPrime-Fluor™ assay kits    

All assays are available as kits
and contract research services.

 

XVPrime-Fluor™ Assay Applications

• Viability, cell functionality, proliferation / cytotoxicity and cell number assays for ex vivo primary explanted cells.
• Cell growth kinetic/expansion studies.
• Developmental biology.
• Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other reagents on primary stem cells and stem cell lines.
• Cell physiology, injury and disease studies.
• Cell-based therapies. 

 

Advantages of using XVPrime-Fluor™

• Uses a fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.
• Non-subjective, instrument-based and quantitative readout procedure.
• Available for adherent or non-adherent stem cells.
• Multiplexes with flow cytometry allowing cells to be detected using other fluorescent labels/markers.
• Multiplexes with luminescence assays.
• Multiplexes with gene expression assays etc for more information from a single sample.
• Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
• High throughput capability.
• Easy to learn, fast to use.

 

Species

• Multiple

 

Cell Sources used with XVPrime-Fluor™

Ex Vivo Primary Explanted Cells from:

 

General and Specialized Cell Systems from Ex Vivo Primary Explanted Cell Sources

• Fibroblastoid
• Epithelial
• Endothelial
• Muscle
• Skeletal
• Kertinocytes
• Melanocytes

 

Please note that for cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

XVPrime-Fluor™ Principle

• Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
• After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time: 2 hours.
• Uses a plate fluorometer or multiparameter plate reader to measure fluorescence with an excitation filter of 380-400nm and an emission filter of 505nm.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Fluorescence plate reader or multiparameter plate reader with an excitation filter of 390-400nm and an emission filter at 505nm.

 

Kit Contents

• GF-AFC Reagent
• Sterile, 96-well plates, depending on assay use
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• XVPrime-Glo™
• XVPrime-Light™
• HALO®-96 Research: bioluminomics™ assays for human and animal lympho-hematopoietic stem cells
• HemoFLUOR™-96: fluorescence assays for lympho-hematopoietic stem cells
• HemoLIGHT™-96: absorbance assays for lympho-hematopoietic stem cells 
• LUMENESC™-96: bioluminomics™ assays for mesenchymal stem cells
• MSCFluor™-96: fluorescence assays for mesenchymal stem cells
• MSCLight™-96: absorbance assays for mesenchymal stem cells 

 

XVPrime-Light™

An Absorbance Assay Platform for Ex Vivo Primary Explanted Cells

 

   Buy XPrime-Light™ assay kits    

All assays are available as kits
and contract research services.

 

XVPrime-Light™ Assay Applications

• Viability, cell functionality, proliferation / cytotoxicity and cell number assays for ex vivo primary explanted cells.
• Cell growth kinetic/expansion studies.
• Developmental biology.
• Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other reagents on primary stem cells and stem cell lines.
• Cell physiology, injury and disease studies.
• Cell-based therapies. 

 

Advantages of using XVPrime-Light™

• Uses a absorbance plate reader or multiparameter plate reader, available in most laboratories.
• Non-subjective, instrument-based and quantitative readout procedure.
• Available for adherent or non-adherent stem cells.
• Multiplexes with gene expression assays etc for more information from a single sample.
• Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
• High throughput capability.
• Easy to learn, fast to use.

 

Species

• Multiple

 

Cell Sources used with XVPrime-Light™

Ex Vivo Primary Explanted Cells from:

 

General and Specialized Cell Systems from Ex Vivo Primary Explanted Cell Sources:

• Fibroblastoid
• Epithelial
• Endothelial
• Muscle
• Skeletal
• Kertinocytes
• Melanocytes

 

Please note that for cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

XVPrime-Light™ Principle

• Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction, colorimetric reaction.
• After culture, just add 20μl of the MTS reagent, mix and read absorbance at 490nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
• No solubilization step as in a MTT reaction. Replaces MTT assays.
• Read results between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Absorbance plate reader or multiparameter plate reader with a 490nm absorbance filter.

 

Kit Contents

• MTS Reagent
• Sterile, 96-well plates, depending on assay use
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• XVPrime-Glo™
• XVPrime-Fluor™
• HALO®-96 Research: bioluminomics™ assays for human and animal lympho-hematopoietic stem cells
• HemoFLUOR™-96: fluorescence assays for lympho-hematopoietic stem cells
• HemoLIGHT™-96: absorbance assays for lympho-hematopoietic stem cells 
• LUMENESC™-96: bioluminomics™ assays for mesenchymal stem cells
• MSCFluor™-96: fluorescence assays for mesenchymal stem cells
• MSCLight™-96: absorbance assays for mesenchymal stem cells 

 

XVPrime-Clone™

A Methylcellulose, Clonal Proliferation and Differentiation Assay for Ex Vivo Primary Explanted Cells using Bioluminomics™ Technology

 

   Buy XPrime-Clone™ assay kits    

All assays are available as kits and contract research services.

 

XVPrime-Clone™ Applications

• To determine stem cell clonogenicity (colony-forming ability).
• To determine the proliferation and determination ability and/or potential into differentiated cells.
• Allows correlation between proliferation and determination ability.
• Subjective clonal (colony-forming) assay standardization.
• Distinguish between growth factors and/or cytokines that have a proliferation and/or differentiation effect on primary (adult) stem cells or stem cell lines.

 

Advantages of using XVPrime-Clone™

• XVPrime-Clone™ allows both proliferation and determination ability and/or potential into differentiated cells to be determined in the same assay.
• The only clonal assay that allows standardization using standards and controls.
• The only assay that allows subjective colony counts to be expressed as standardized ATP concentrations.
• Incorporate proven bioluminomics™ technology first developed in the CAMEO™-96 Platform.
• Multiplexing capability with other assays allows for more information to be obtained from the same sample.

 

Species

• Multiple

 

Cell Sources used with XVPrime-Clone™

Ex Vivo Primary Explanted Cells from:

 

General and Specialized Cell Systems from Ex Vivo Primary Explanted Cell Sources:

• Fibroblastoid
• Epithelial
• Endothelial
• Muscle
• Skeletal
• Kertinocytes
• Melanocytes

 

Please note that for cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents

• XVPrime-Clone™ Methylcellulose Base Reagent 
• ATP standard
• ATP controls
• ATP Enumeration Reagent
• Sterile, 96-well plates
• Non-sterile, 96-well plates
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• STEMClone™
• CAMEO™-96

 

HepatoGLO™-HT

A Bioluminomics™ Assay Platform for Hepatocytes and Hepatotoxicity Screening and Testing

 

   Buy HepatoGlo™-HT assay kits    

This assay is available as assay kits
and contract research services.

 

HepatoGLO™-HT Applications

• Hepatocytes have high intracellular concentrations of ATP. Changes in hepatocyte metabolism can be detected and measured using HepatoGLO™.
• Specifically designed to screen and test for hepatotoxicity.
• High throughput in vitro ADME/Tox screening.
• Drug/agent toxicity ranking according to organ/tissue and species.

 

Advantages of using HepatoGLO™-HT

• Incorporates proven ATP bioluminomics™ technology, the most sensitive signal detection system available.
• Fully calibrated and standardized assay that can be validated according to regulatory requirements.
• Reliably compared over time.
• Available for adherent or non-adherent cells.
• Allows for the flexibility to use virtually any hepatocyte culture protocol, but include HepatoGro™ Medium.
• Designed for multiplexing with other assays to produce the most amount of information from a single sample.
• Rapid turnaround.
• Easy to learn, fast to use. 

 

Species

• Human
• Non-human primate
• Dog
• Rat
• Mouse
• Please inquire for other species

 

Hepatocytes

• Fresh
• Cryopreserved
• Male
• Female
• Pooled
• Hepatocyte cell lines 

 

Examples using HepatoGLO™-HT

The two diagrams to the left compare the response of 5-fluorouracil and doxorubicin on cryopreserved human and rat hepatocytes. Notice the different scale on the Y-axis for the two species. Although hepatocytes do not represent a proliferating cell population under normal steady-state conditions, they are actively producing intracellular ATP. The difference in normal ATP production is shown on the Y-axis. For the examples shown here, HepatoGlo™-HT provides an reliable high throughput assay platform to detect hepatotoxicity during ADME/Tox screening. Furthermore, the HepatoGLO™-DDI is a perfect addition to the hepatotoxicity screening platform for drug-drug interaction. Please contact HemoGenix® for more information. 

 

 

 

 

Availability

HepatoGlo™-HT assays are available with:

• 96- or 384-well plates

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents

• HepatoGro™ Medium
• ATP standard
• ATP controls
• ATP Enumeration Reagent
• Sterile, 96- or 384-well plates, depending on assay use
• Non-sterile, 96- or 384-well plates, depending on assay use
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual