
XVPrime™-PRT
An ATP Bioluminomics™ Predictive Residual Toxicity Assay for Ex Vivo Primary Explanted Cells
Buy XPrime™-PRT assay kits
All assays are available as kits
and contract research services.
XVPrime™-PRT Assay Applications
- To determine the proportion of cells remaining or resistant to drug administration.
- To determine whether cells change their sensitivity to a drug after a single dose.
- To determine the effect on cells to multiple drug administration.
- To determine whether cells exhibit expansion potential after a single drug dose.
Advantages of using XVPrime™-PRT
- In vitro assay that detects whether unaffected cells (residual cells) remain after drug administration.
- In vitro assay that indicates changes in drug sensitivity to cells.
- The most sensitive of any assay readouts incorporating proven ATP Bioluminomics™ Technology.
- Instrument-based, quantitative and fully standardized readout procedure.
- Available for adherent or non-adherent cells.
- Flexibility to use virtually any in vitro cell protocol, reagents and growth factors/cytokines.
- Reliably compare results over time.
- Incorporate secondary, re-plating technology.
- Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
- Easy to learn, fast to use.
Species
Cell Sources used with XVPrime-Glo™ or XVPrime-Glo™ HT
Ex Vivo Primary Explanted Cells from:
- Liver
- Kidney
- Heart
- Lung
- Bladder
- Gut
- Skin
- Ovary
- Testis
- Breast
- Prostate
- Brain
General and Specialized Cell Systems from Ex Vivo Primary Explanted Cell Sources:
- Fibroblastoid
- Epithelial
- Endothelial
- Muscle
- Skeletal
- Kertinocytes
- Melanocytes
Please note that for cells of the blood-forming system, HemoGenix® has developed specialized assay platforms. See Related Assays below.
Use
For Research Use Only. Not for clinical diagnostic use.
Major Equipment Required
Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.
Kit Contents
- ATP standard
- ATP controls
- ATP Enumeration Reagent
- 2 or 4, sterile, 96-well plates
- Non-sterile, 96-well plates
- Sterile, adhesive foil covers to maintain sterility of unused wells
- Instruction manual
Related Assays