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  •  XVPrime™-PRT

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XVPrime™-PRT

An ATP Bioluminomics™ Predictive Residual Toxicity Assay for Ex Vivo Primary Explanted Cells

 

   Buy XPrime™-PRT assay kits    

All assays are available as kits
and contract research services.

 

XVPrime™-PRT Assay Applications
  • To determine the proportion of cells remaining or resistant to drug administration.
  • To determine whether cells change their sensitivity to a drug after a single dose.
  • To determine the effect on cells to multiple drug administration.
  • To determine whether cells exhibit expansion potential after a single drug dose.

 

Advantages of using XVPrime™-PRT
  • In vitro assay that detects whether unaffected cells (residual cells) remain after drug administration.
  • In vitro assay that indicates changes in drug sensitivity to cells.
  • The most sensitive of any assay readouts incorporating proven ATP Bioluminomics™ Technology.
  • Instrument-based, quantitative and fully standardized readout procedure.
  • Available for adherent or non-adherent cells.
  • Flexibility to use virtually any in vitro cell protocol, reagents and growth factors/cytokines.
  • Reliably compare results over time.
  • Incorporate secondary, re-plating technology.
  • Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
  • Easy to learn, fast to use.

 

Species
  • Multiple

 

Cell Sources used with XVPrime-Glo™ or XVPrime-Glo™ HT

Ex Vivo Primary Explanted Cells from:

  • Liver
  • Kidney
  • Heart
  • Lung
  • Bladder
  • Gut
  • Skin
  • Ovary
  • Testis
  • Breast
  • Prostate
  • Brain

 

General and Specialized Cell Systems from Ex Vivo Primary Explanted Cell Sources:

  • Fibroblastoid
  • Epithelial
  • Endothelial
  • Muscle
  • Skeletal
  • Kertinocytes
  • Melanocytes

 

Please note that for cells of the blood-forming system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • 2 or 4, sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 

Related Assays

 

Hemogenix Inc.