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Products - STEMAssays    Page   1  |  2  | 
 
  • STEMGlo™: Cell

    Proliferation Assays

  •  STEMClone™:

    Clonal Assays

  • StemGlo™-Potency:

    Cell Potency Assays

  • STEMGlo™-Tox HT:

    Toxicity Assays

  •  STEMGlo™-PRT:

    Residual Toxicity Assays

STEMGlo™

 

A Flexible, Standardized and Validated 96-Well, Bioluminescence Assay Kit to Measure Proliferation, Viability and Functionality of ES and iPS Cells, Primary Explanted Cells, Transformed Cell Lines and Cancer Cells.

 

GO TO Preferred Cell Systems

 

 

Uses of STEMGlo™
  • Cell proliferation, viability, cell functionality and cell number assays for multiple cell types derived from different organs, tissues and species.
  • Developmental biology.
  • Studies on the cell proliferation process.
  • Determination of stem cells into differentiated cells.
  • Cell-based therapies. 
  • Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other factors on multiple cell types and cell lines.
  • Stem cell gene targeting and expression research, e.g. using CRISPR-Cas9.

 

Benefits of using STEMGlo™-96 Research
  • The most sensitive and accurate assay readout available incorporating proven ATP bioluminescence technology.
  • Instrument-based, quantitative and fully standardized readout procedure.
  • Available for adherent or non-adherent stem cells.
  • 2D and 3D cell culture capability.
  • Flexibility to use virtually any in vitro cell protocol, reagents and growth factors/cytokines.
  • Can be adapted for cellular therapy or regenerative medicine quality control and potency testing.
  • High throughput capability.
  • Reliably compare results over time.
  • Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
  • Fast to learn, easy to use and implement.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species and Cell Sources
  • Multiple

Cell Sources

STEMGlo™-96 Research can be used to study virtually any cell type with proliferation capability or high intracellular ATP concentrations. These include, but are not limited to cells from the following organs and tissues:

  • Bladder
  • Brain
  • Breast
  • Eye
  • Gut
  • Heart
  • Kidney
  • Lung
  • Liver
  • Ovary
  • Prostate
  • Skin
  • Embryonic tissue
  • Fetal tissue.

Other cell types that can be studies using STEMGlo™-96 Research include:

  • Proliferating cell lines
  • Tumor cells
  • Embryonic stem (ES) cells
  • Induced puripotent stem (iPS) cells
  • ES-or iPS-derived cells

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use. 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
Kit Contents
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates, depending on assay use
  • Non-sterile, 96-well plates, depending on assay use
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 STEMGlo™-96 Research Quick Guide and Assay Manual

Download the STEMGlo™-96 Research Quick Guide

The STEMGlo™-96 Research Assay Manual can be accessed from the Resources section here

STEMClone™

A Standardized Methylcellulose, 96-Well Bioluminescence Clonal Proliferation and Differentiation Assay for ES and iPS Cells, Primary Cells, Transformed Cell Lines and Cancer Cells

 

GO TO Preferred Cell Systems

 

 

 
Uses of STEMClone™
  • To determine cell clonogenicity (colony-forming ability).
  • To determine the proliferation and determination ability and/or potential into differentiated cells from primary (adult) stem cells or stem cell lines.
  • Allows correlation between proliferation and determination ability.
  • Clonal (colony-forming) assay standardization.
  • Distinguish between growth factors and/or cytokines that have a proliferation and/or differentiation effect on primary (adult) stem cells or stem cell lines.

 

Benefits of using STEMClone™
  • STEMClone™ is the only assay that allows both proliferation and determination ability and/or potential into differentiated cells to be determined in the same assay.
  • The only clonal assay incorporating standards and controls.
  • The only assay that allows subjective colony counts to be expressed as standardized ATP concentrations.
  • Incorporate proven ATP bioluminescence technology first developed in the CAMEO™-96 Platform.
  • Multiplexing capability with other assays allows for more information to be obtained from the same sample.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species and Cell Sources
  • Multiple

Clonal Stem Cell Sources
  • Primary (adult) stem cells derived from organ and tissue sources
  • Primary cancer stem cells 
  • Embryonic stem (ES) cells
  • Induced pluripotent stem (iPS) cells
  • Primordial germ cells (PGC)
  • Other stem cell lines

Organs and Tissues from which Cells Demonstrate Clonality
  • Bladder
  • Breast
  • Heart
  • Lung
  • Prostate
  • Skin
  • Embryonic tissue
  • Fetal tissue.

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
  • STEMClone™ Methylcellulose Base Reagent
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 STEMClone™ Quick Guide and Assay Manual

Download the STEMClone™ Quick Guide

The STEMClone™ Assay Manual can be accessed from the Resources section here

 Related Assays
Related Assays

STEMGlo™ Potency 

A Standardized and Validated 96-Well, Bioluminescence Potency and Quality Assay Kit to Release Stem Cell Products for Use 

 

GO TO Preferred Cell Systems

 

 

Articles on stem cell potency

Click image for larger view

 

What is Stem Cell Potency?
  • Stem cell potency is the quantitative and validated measurement of proliferation potential or capacity (biological activity) of the "active" stem cell components to produce the intended effect or response.
  • Stem cell proliferation potential or capacity correlates directly with stem cell self-renewal and primitiveness or "stemness". Thus, the greater the proliferation potential, the greater the self-renewal capacity and the more primitive the stem cell population. The more primitive a stem cell population, the greater its potency.
  • Potency must correlate with a response, but not necessarily the clinical outcome.

 

Application / Research Areas
  • Stem cells
  • Cell potency
  • Cellular therapy
  • Regenerative medicine
  • Cell proliferation
  • Cell viability

 

Uses of STEMGlo™-Potency
  • To determine stem cell potency and quality in a single assay for any stem cell product used for a therapeutic application. (See HALO®-Potency and MSCGlo™-Potency for hematopoietic and mesenchymal stem/stromal cell potency assays).
  • To use both stem cell potency and quality to define release criteria and determine if a product can be released for use.
  • To establish in-house reference standards that allow the potency ratio (measure of potency) to be determined.
  • To use an FDA complient potency assay for stem cell therapy products.
  • To promote "Best Practice Criteria Testing". 

 

Benefits of using STEMGlo™-Potency Prior to Using a Stem Cell Product
  • STEMGlo™-Potency is the only commercially available, reference standard-based stem cell potency assay designed for use with ES-, iPS or primary stem cell therapy products.
  • STEMGlo™-Potency reliably estimates the stem cell potency ratio and quality of one or more stem cell populations simultaneously against a reference standard to predict whether the product will grow prior to use.
  • STEMGlo™-Potency uses the standardized 'Slope-Ratio Concentration-Response Model" for measuring potency as described in the U.S. Pharmacopeia.
  • Requires as few as 90,000 cells to perform the assay.
  • Instrument-based, incorporating an external standard and controls for assay calibration, standardization and validation according to FDA regulations.
  • Allows for intra- and inter-laboratory comparisons.
  • Assay can be completed in 4-7 days depending on the cells being tested.
  • STEMGlo™-Potency can help any laboratory achieve "Best Practices" and measurement assurance promoted by the National Institute of Standards and Technology (NIST) .

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 The Stem Cell Reference Standard
  • To measure potency, a reference standard (RS) of the same material and purity as the sample is required so that the potency ratio can be determined. The inclusion of a RS to measure potency is an FDA requirement. Universal or global reference standards for stem cell products are not available. As a result, it is necessary for individual centers to establish their own in-house RS. To accomplish this, a standardized and validated assay platform is required.
  • STEMGlo™-Potency can be used to establish reference standards. A batch of stem cells is prepared and processed to the required purity, aliquoted and cryopreserved and it's potency determined using the STEMGlo™-Potency assay. Other secondary and tertiary RSs are also prepared. The RS is then used to compare to the sample of unknown potency.

 Availability of STEMGlo™-Potency Assays

 

STEMGlo™-Potency Assays Kits Available to Determine

Stem Cell Potency, Quality and Release

Assay Type Cell Type Populations Measured (*) Catalog Number Number of Plates/Kit

Number of Samples/Kit

Click to see price

STEMGlo™-Potency

Any ES, iPS or primary stem cell population Preferably a minimum of 2

KSG-PQR-1

1

1 + RS

Price
STEMGlo™-Potency Any ES, iPS or primary stem cell population Preferably a minimum of 2

KSG-PQR-2

2

2 + 2 RS(1) or

3 + 1 RS(1)

Price
STEMGlo™-Potency Any ES, iPS or primary stem cell population Preferably a minimum of 2

KSG-PQR-4

4

6 + 1 RS(1) or 

4 + 4 RS(1)

Price

(1): One RS must always be performed when an unknown sample is measured. If more than 1 sample is to be measured, a single RS of the same cell type and purity can be used to compare with all samples performed on the same day.

 Tissues and Cell Purity Required for Assay
  • Embryonic stem (ES) cells
  • Induced pluripotent stem (iPS) cells
  • Primary stem cells obtained from any tissue or organ. PLEASE NOTE that separte potency assays are available for hematopoietic and mesenchymal stem/stromal cells.

Stem Cell Purity Requirements for Detection

For best results, it is recommended that as few cell impurities as possible are present, since these will mask and dilute the stem cells producing underestimates for the actual stem cell potency and quality. 

 Example of How Potency is Measured

See information on HALO®-Potency to see how potency is measured in vitro.

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use

Major Equipment Required

A plate luminometer or multiparameter plate reader capable of measuring "glow" luminescence is needed for this assay.

 Assay Kit Contents
  • Base medium for diluting ATP standard
  • ATP standards
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plate(s)
  • Non-sterile, 96-well plate(s)
  • Sterile, adhesive foil covers
  • Assay manual

 STEMGlo™-Potency Quick Guide and Assay Manual

Download the STEMGlo™-Potency Quick Guide

The STEMGlo™-Potency Assay Manual can be accessed from the Resources section here

STEMGlo™-Tox HT

ATP Bioluminescence™ In Vitro Cytotoxicity Assays for Primary Cells, Stem Cells and other Cell Lines

 

 

GO TO Preferred Cell Systems

 

 

 
Uses of StemGlo™-Tox HT
  • High throughput in vitro toxicity screening and testing of multiple cell types with multiplexing capability.
  • Can be incorporated at any stage of drug development, including ADME/Tox.
  • Drug/agent toxicity ranking according to cell source and species.
  • Development of drugs and other therapies using stem cell gene targeting and expression research, e.g. CRISPR-Cas9.

 

Benefits of using STEMGlo™-Tox HT

  • A flexible, in vitro assay platform to perform high throughput toxicity testing.
  • Can be applied to numerous cell types derived from different organs, tissues and species.
  • High-throughput capability using 96- or 384-well plate formats allows ADME-Tox drug or compound screening, thereby significantly reducing unexpected results during pre-clinical testing.
  • Available for adherent or non-adherent cell types.
  • 2D and 3D cell culture models.
  • STEMGlo™-Tox HT is part of the ComparaTox™ 1 Platform to compare and rank toxicities from different organs, tissues and species. (Please see separate information flyer).
  • Incorporates the most sensitive ATP bioluminescence readout available to measure proliferation, cytotoxicity, cell number and even apoptosis.
  • All assay kits include standards and controls to calibrate and standardize the assay prior to measuring samples.
  • Validated signal detection readout according to FDA Bioanalytical Method Guidelines.
  • Allows results to be compared over time.
  • Results usually in 4 to 5 days depending on species and cell type.
  • Designed for multiplexing with other assay using the same sample.
  • STEMGlo™-Tox HT assay kits include everything required to perform the assay, except cells.
  • STEMGlo™-96 PRT detects predictive residual toxicity after a single dose.
  • All assays are fast to learn and easy to use and implement.
  • A 3Rs Assay Platform-Helps Reduction, Refinement, Replacement for animal testing.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species and Cell Sources
  • Multiple

Cell Types for use with STEMGlo™-Tox HT

STEMGlo™-Tox HT can be used with cell types from the following organs and tissues:

  • Bladder
  • Brain
  • Breast
  • Eye
  • Gut
  • Heart
  • Kidney
  • Lung
  • Liver
  • Ovary
  • Prostate
  • Skin
  • Embryonic tissue
  • Fetal tissue

Other cell types include:

  • Proliferating cell lines
  • Tumor cells
  • Embryonic stem (ES) cells
  • Induce pluripotent stem (iPS) cells

For hepatotoxicity and neurotoxicity, specific assays are available. See HepatoGo™-Tox HT and NeutoGlo™-Tox HT.

 Availability of STEMGlo™-Tox HT

In vitro toxicity screening and testing using either 96- or 384-well plates

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
Kit Contents
  • ATP standard
  • ATP controls
  • Medium
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well plates, depending on assay use
  • Non-sterile, 96- or 384-well plates, depending on assay use
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 ASTEMGlo™-Tox HT Quick Guide and Assay Manual

Download the STEMGlo™-Tox HT Quick Guide

The STEMGlo™-Tox HT Assay Manual can be accessed from the Resources section here

 Related assays

 

STEMGlo™ PRT

ATP Bioluminomics™ Predictive Residual Toxicity Assays for Stem Cells

 

GO TO Preferred Cell Systems

 

 

 
Uses of STEMGlo™ PRT
  • To determine the proportion of cells remaining or resistant to drug administration.
  • To determine whether cells change their sensitivity to a drug after a single dose.
  • To determine the effect of multiple drug administration.
  • To determine whether cells exhibit expansion potential after a single drug dose.

 

Benefits of using STEMGlo™ PRT
  • In vitro assay that detects whether residual (unaffected) cells remain after drug administration.
  • In vitro assay that indicates changes in drug sensitivity to cells.
  • The most sensitive of any assay readouts incorporating proven ATP bioluminescence technology.
  • Instrument-based, quantitative and fully standardized readout procedure.
  • Available for adherent or non-adherent stem cells.
  • Flexibility to use virtually any in vitro cell protocol, reagents and growth factors/cytokines.
  • Reliably compare results over time.
  • Incorporates secondary, re-plating technology.
  • Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
  • Fast to learn and easy to use.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species and Cell Sources
  • Multiple

Stem Cell Sources
  • STEMGlo™ PRT utilizes the same cell types as STEMGlo™-Tox HT.

 Use and Major equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
Kit Contents
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • 2, sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 STEMGlo™ PRT Quick Guide and Assay Manual

Download the STEMGlo™ PRT Quick Guide

The STEMGlo™ PRT Assay Manual can be accessed from the Resources section here

 Related Assays
Related Assays