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Products - Stem Assays    Page   1  |  2  | 

  • STEMGlo™ and STEMGlo™-Tox HT

  •  STEMFluor™

  •  STEMLight™

  •  STEMClone™

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STEMGlo™ and STEMGlo™-Tox HT

An ATP Bioluminomics™ Assay Platform for Primary (Adult) Stem Cells and Stem Cell Lines

       Buy STEM-Glo™ assay kits       

 

Buy STEM-Glo™-Tox HT assay kits Available as assay kits and
contract research services.

 

STEMGlo™ Assay Applications
  • Viability, cell functionality, proliferation / cytotoxicity and cell number assays for primary (adult) stem cells and stem cell lines (e.g. ES and iPS cells).
  • Developmental biology.
  • Studies on the stem cell proliferation process.
  • Determination of stem cells into differentiated cells.
  • Cell-based therapies. 
  • Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other factors on primary (adult) stem cells and stem cell lines.
  • Comparison of ES- or iPS-derived stem cell response with primary stem cells.

 

Special StemGlo™-Tox HT Applications
  • High throughput in vitro toxicity screening and testing.
  • Can be incorporated at any stage of drug development, including ADME/Tox.
  • Drug/agent toxicity ranking according to stem cell source and species.

 

Advantages of using STEMGlo™
  • The most sensitive of any assay readouts incorporating proven Bioluminomics™ Technology.
  • Instrument-based, quantitative and fully standardized readout procedure.
  • Available for adherent or non-adherent stem cells.
  • Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
  • Can be used for stem cell quality control and potency testing.
  • High throughput capability.
  • Reliably compare results over time.
  • Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
  • Easy to learn, fast to use.

 

Special Advantages of using STEMGlo™-Tox HT

In addition to the advantages of using STEMGlo™, the high throughput in vitro toxicity platform has the following special advantages:

  • A 3Rs alternative in vitro assay platform for Reduction, Refinement and Replacement of animal testing.
  • 96- and 384-well plate availability.
  • Rapid turnaround.
  • Complete assay calibration, standardization and validation according to regulatory requirements.

 

Species
  • Multiple

 

Stem Cell Sources
  • Primary (adult) stem cells derived from organ and tissue sources
  • Primary cancer stem cells 
  • Embryonic stem (ES) cells
  • Induced pluripotent stem (iPS) cells
  • Primordial germ cells (PGC)
  • Other stem cell lines

Please note that for stem cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

Availability

STEMGlo™ assays are available for:

  • Stem cell research with 96-well plates
  • In vitro toxicity screening and testing with 96- or 384-well plates

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well plates, depending on assay use
  • Non-sterile, 96- or 384-well plates, depending on assay use
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 

Related Assays

 

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STEMFluor™

A Fluorescence Assay Platform for Primary (Adult) Stem Cells and Stem Cell Lines

 

   Buy STEMFluor™ assay kits    

All assays are available as kits
and contract research services.

 

STEMFluor™ Assay Applications
  • Viability, cell functionality, proliferation / cytotoxicity and cell number assays for primary (adult) stem cells and stem cell lines (e.g. ES and iPS cells).
  • Developmental biology.
  • Determine self-renewal and expansion ability and potential.
  • Studies on the stem cell proliferation process.
  • Determination of stem cells into differentiated cells.
  • Cell-based therapies. 
  • Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other factors on primary (adult) stem cells and stem cell lines.
  • Comparison of ES- or iPS-derived stem cell response with primary stem cells.

 

Advantages of using STEMFluor™
  • Uses a fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.
  • Non-subjective, instrument-based and quantitative readout procedure.
    Available for adherent or non-adherent stem cells.
  • Multiplexes with flow cytometry allowing cells to be detected using other fluorescent labels/markers.
  • Multiplexes with luminescence assays.
  • Multiplexes with gene expression assays etc for more information from a single sample.
  • Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
  • High throughput capability.
  • Easy to learn, fast to use.

 

Species
  • Multiple

 

Stem Cell Sources
  • Primary (adult) stem cells derived from organ and tissue sources
  • Primary cancer stem cells 
  • Embryonic stem (ES) cells
  • Induced pluripotent stem (iPS) cells
  • Primordial germ cells (PGC)
  • Other stem cell lines

Please note that for stem cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

STEMFluor™-96 Principle
  • Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
  • After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time: 2 hours.
  • Uses a plate fluorometer or multiparameter plate reader to measure fluorescence with an excitation filter of 380-400nm and an emission filter of 505nm.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Fluorescence plate reader or multiparameter plate reader.

 

Kit Contents
  • GF-AFC Reagent
  • Sterile, 96-well plates, depending on assay use
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 

Related Assays

 

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STEMLight™

An Absorbance Assay Platform for Primary (Adult) Stem Cells and Stem Cell Lines

 

   Buy STEMLight™ assay kits    

All assays are available as kits
and contract research services.

 

STEMLight™ Assay Applications
  • Viability, cell functionality, proliferation / cytotoxicity and cell number assays for primary (adult) stem cells and stem cell lines (e.g. ES and iPS cells).
  • Developmental biology.
  • Determine self-renewal and expansion ability and potential.
  • Studies on the stem cell proliferation process.
  • Determination of stem cells into differentiated cells.
  • Cell-based therapies. 
  • Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other factors on primary (adult) stem cells and stem cell lines.
  • Comparison of ES- or iPS-derived stem cell response with primary stem cells.

 

Advantages of using STEMLight™
  • Uses a absorbance plate reader or multiparameter plate reader, available in most laboratories.
  • Non-subjective, instrument-based and quantitative readout procedure.
    Available for adherent or non-adherent stem cells.
  • Multiplexes with gene expression assays etc for more information from a single sample.
  • Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
  • High throughput capability.
  • Easy to learn, fast to use.

 

Species
  • Multiple

 

Stem Cell Sources
  • Primary (adult) stem cells derived from organ and tissue sources
  • Primary cancer stem cells 
  • Embryonic stem (ES) cells
  • Induced pluripotent stem (iPS) cells
  • Primordial germ cells (PGC)
  • Other stem cell lines

Please note that for stem cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

STEMLight™-96 Principle
  • Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction, colorimetric reaction.
  • After culture, just add 20μl of the MTS reagent, mix and read absorbance at 490nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.
  • Read results between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Absorbance plate reader or multiparameter plate reader with a 490nm filter.

 

Kit Contents
  • MTS Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plate for background determination
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 

Related Assays

 

STEMAssays_mini.jpg

STEMClone™

A Methylcellulose, Clonal Proliferation and Differentiation Assay for Primay (Adult) Stem Cells and Stem Cell Lines Incorporating Bioluminomics™ Technology

 

   Buy STEMClone™ assay kits    

All assays are available as kits
and contract research services.

 

STEMClone™ Applications
  • To determine stem cell clonogenicity (colony-forming ability).
  • To determine the proliferation and determination ability and/or potential into differentiated cells from primary (adult) stem cells or stem cell lines.
  • Allows correlation between proliferation and determination ability.
  • Subjective clonal (colony-forming) assay standardization.
  • Distinguish between growth factors and/or cytokines that have a proliferation and/or differentiation effect on primary (adult) stem cells or stem cell lines.

 

Advantages of using STEMClone™
  • STEMClone™ is the only assay that allows both proliferation and determination ability and/or potential into differentiated cells to be determined in the same assay.
  • The only clonal assay that allows standardization using standards and controls.
  • The only assay that allows subjective colony counts to be expressed as standardized ATP concentrations.
  • Incorporate proven bioluminomics technology first developed in the CAMEO™-96 Platform.
  • Multiplexing capability with other assays allows for more information to be obtained from the same sample.

 

Species
  • Multiple

 

Stem Cell Sources
  • Primary (adult) stem cells derived from organ and tissue sources
  • Primary cancer stem cells 
  • Embryonic stem (ES) cells
  • Induced pluripotent stem (iPS) cells
  • Primordial germ cells (PGC)
  • Other stem cell lines

Please note that for stem cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents
  • STEMClone™ Methylcellulose Base Reagent
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plates
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 

Related Assays

 

 

 

Hemogenix Inc.