NeuroGLO™-Complete &
NeuroGLO™-Complete HT

A Complete "Turnkey" ATP Bioluminomics™ Assay Platform for ES-Derived Neural or Neuronal Cells
 

Available as assay kits and contract research services.

Buy Neuro-Glo™ Complete assay kits

Buy Neuro-Glo™-Complete HT assay kits

 

NeuroGlo™ Complete HT is a collaboration between ArunA Biomedical and HemoGenix® 

NeuroGLO™ Complete Assay Applications

• Viability, cell functionality, proliferation / cytotoxicity and cell number assays of ES-derived neural cells.
• Developmental biology.
• Determine expansion ability and potential.
• Neural proliferation and differentiation process.
• Comparison of ES-derived cells with primary cells.
• High throughput in vitro toxicity testing and screening.

 

Advantages of using NeuroGLO™ Complete

• Incorporates the most sensitive of any assay readout using proven ATP-based Bioluminomics™ Technology.
• Instrument-based, quantitative and fully standardized readout procedure that can be validated according to regulatory requirements.
• Includes cryopreserved hNP1 human neural progenitor cells or hN2 neuronal cells, medium, plates and signal detection system for a complete "turnkey" assay. 
• High throughput capability.
• Reliably compare results over time.
• Multiplexing capability to incorporate other assay readouts for additional information from the same sample.
• Easy to learn, fast to use.

 

Species

• Human

 

Stem Cell Sources

• Embryonic stem (ES) cell-derived hNP1 neural stem/progenitor cells

 

Availability

NeuroGLO™ Complete assays are available for:

• Stem cell research assays with 96-well plates
• In vitro toxicity screening and testing with 96- or 384-well plates

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents

• Cryopreserved vial(s) of hNP1 neural progenitor or hN2 neuronal cells
• Growth/expansion medium
• ATP standard
• ATP controls
• ATP Enumeration Reagent
• Sterile, 96- or 384-well plates, depending on assay use
• Non-sterile, 96- or 384-well plates, depending on assay use
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• LUMENESC™ Complete: Bioluminomics™ assays for mesenchymal stem cells

 

STEMGlo™ PREP

An ATP Bioluminomics™ Assay to Detect Proliferation, Self-Renewal and Expansion Potential of Primary (Adult) Stem Cells and Stem Cell Lines

 

   Buy STEMGlo™ PREP assay kits    

All assays are available as kits
and contract research services.

 

STEMGlo™ PREP Assay Applications

• Designed to help substantiate the presence of stem cells by using secondary re-plating culture technology.
• Determine self-renewal and expansion ability and potential of primary (adult) stem cells and stem cell lines.
• Determine the effects of growth factors/cytokines and other factors on primary (adult) stem cells and stem cell line capability.
• Comparison of self-renewal and expansion potential of ES- or iPS-derived stem cell response with primary stem cells.
• Distinguish between quiescent and proliferating stem cells.
• Use to "prime" stem cells out of quiescence and into cell cycle or visa versa.

 

Advantages of using STEMGlo™ PREP

• The most sensitive of any assay readouts incorporating proven ATP Bioluminomics™ Technology.
• Instrument-based, quantitative and fully standardized readout procedure.
• Available for adherent or non-adherent stem cells.
• Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
• Can be used for stem cell quality control and potency testing.
• Reliably compare results over time.
• Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
• Easy to learn, fast to use.

 

Species

• Multiple

 

Stem Cell Sources

• Primary (adult) stem cells derived from organ and tissue sources
• Primary cancer stem cells 
• Embryonic stem (ES) cells
• Induced pluripotent stem (iPS) cells
• Primordial germ cells (PGC)
• Other stem cell lines

Please note that for stem cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents

• ATP standard
• ATP controls
• ATP Enumeration Reagent
• 2 or 4 sterile, 96-well culture plates
• Non-sterile, 96-well plates
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• HALO®-96 PREP: to detect self-renewal and expansion potential of lympho-hematopoietic stem cells

 

STEMGlo™-PRT

An ATP Bioluminomics™ Predictive Residual Toxicity Assay for Primary (Adult) Stem Cells & Stem Cell Lines

 

   Buy STEMGlo™-PRT assay kits    

All assays are available as kits
and contract research services.

 

STEMGlo™-PRT Assay Applications

• To determine the proportion of stem cells remaining or resistant to drug administration.
• To determine whether stem cells change their sensitivity to a drug after a single dose.
• To determine the effect on primitive stem cells to multiple drug administration.
• To determine whether stem cells exhibit expansion potential after a single drug dose.

 

Advantages of using STEMGlo™-PRT

• In vitro assay that detects whether unaffected stem cells remain after drug administration.
• In vitro assay that indicates changes in drug sensitivity to stem cells.
• The most sensitive of any assay readouts incorporating proven ATP Bioluminomics™ Technology.
• Instrument-based, quantitative and fully standardized readout procedure.
• Available for adherent or non-adherent stem cells.
• Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
• Reliably compare results over time.
• Incorporate secondary, re-plating technology.
• Multiplexing capability to incorporate flow cytometry and other assay readouts for additional information from the same sample.
• Easy to learn, fast to use.

 

Species

• Multiple

 

Stem Cell Sources

• Primary (adult) stem cells derived from organ and tissue sources
• Primary cancer stem cells 
• Embryonic stem (ES) cells
• Induced pluripotent stem (iPS) cells
• Primordial germ cells (PGC)
• Other stem cell lines

Please note that for stem cells of the blood-forming system and the mesenchymal stem cell system, HemoGenix® has developed specialized assay platforms. See Related Assays below.

 

Use

For Research Use Only. Not for clinical diagnostic use.

 

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 

Kit Contents

• ATP standard
• ATP controls
• ATP Enumeration Reagent
• 2 or 4, sterile, 96-well plates
• Non-sterile, 96-well plates
• Sterile, adhesive foil covers to maintain sterility of unused wells
• Instruction manual

 

Related Assays

• HALO®-96 PRT