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Products - STEMAssays    Page   1  |  2  | 
 
  • HepatoGLO™-HT:

    Hepatotoxicity Assays

  • NeuroGLO™-Tox HT:

    Neurotoxicity Assays

  • STEMFluor™: Cell

    Proliferation Assays

  • STEMLight™: Cell

    Proliferation Assays

HepatoGLO™-Tox HT

A Standardized and Validated 96- and 384-Well Bioluminescence Assay for Hepatocytes and Hepatotoxicity Screening and Testing

 

   Buy HepatoGLO™-Tox HT Assay Kits    

 

 

 
Uses of HepatoGLO™-Tox HT
  • Hepatocytes have high intracellular concentrations of ATP. Changes in hepatocyte metabolism can be detected and measured using HepatoGLO™.
  • Specifically designed to screen and test for hepatotoxicity.
  • High throughput in vitro ADME/Tox screening.
  • Drug/agent toxicity ranking according to organ/tissue and species.

 

Benefits of using HepatoGLO™-Tox HT
  • Incorporates proven ATP bioluminomics™ technology, the most sensitive signal detection system available.
  • Fully calibrated and standardized assay that can be validated according to regulatory requirements.
  • Reliably compare results over time.
  • Available for adherent or non-adherent cells.
  • Allows for the flexibility to use virtually any hepatocyte culture protocol, but include HepatoGro™ Medium.
  • Designed for multiplexing with other assays to produce the most amount of information from a single sample.
  • Rapid turnaround.
  • Easy to learn, fast to use. 

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Hepatocytes: Species and Types
Species
  • Human
  • Non-human primate
  • Dog
  • Rat
  • Mouse
  • Please inquire for other species

Hepatocytes
  • Fresh
  • Cryopreserved
  • Male
  • Female
  • Pooled
  • Induce pluripotent stem (iPS) cell-derived hepatocytes

 Examples using HepatoGLO™-Tox HT

HepatoGlo5FU

Click image for larger view

HepatoGloDox

Click image for larger view

The two diagrams to the left compare the response of 5-fluorouracil and doxorubicin on cryopreserved human and rat hepatocytes. Notice the different scale on the Y-axis for the two species. Although hepatocytes do not represent a proliferating cell population under normal steady-state conditions, they are actively producing intracellular ATP. The difference in normal ATP production is shown on the Y-axis. For the examples shown here, HepatoGlo™-HT provides an reliable high throughput assay platform to detect hepatotoxicity during ADME/Tox screening. Furthermore, the HepatoGLO™-DDI is a perfect addition to the hepatotoxicity screening platform for drug-drug interaction. Please contact HemoGenix® for more information. 

 Availability
Availability

HepatoGLO™-Tox HT assays are available with:

  • 96- or 384-well plates

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
  • HepatoGro™ Medium
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well plates, depending on assay use
  • Non-sterile, 96- or 384-well plates, depending on assay use
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 HepatoGLO™-Tox HT Quick Guide and Assay Manual

Download the HepatoGLO™-Toc HT Quick Guide

The HepatoGLO™-Tox HT Assay Manual can be found in the Resources section here

NeuroGlo™-Tox HT
NeuroGlo™ Complete

A Standardized and Validated 96- and 384-Well Bioluminescence Assay for ES-Derived Neural and Neuronal Cell Screening and Testing
 

 

       Buy NeuroGlo-Complete Assays       

 

Buy NeuroGlo™-Tox HT Assays

 

NeuroGlo™ Contract Services

 

 

NeuroGlo™ Complete HT is a collaboration between ArunA Biomedical and HemoGenix® 

 
 
Uses of NeuroGLO™-Tox HT and NeuroGlo™-Complete
  • Viability, cell functionality, proliferation / cytotoxicity and cell number assays of ES-derived neural cells.
  • Developmental biology.
  • Determine expansion ability and potential.
  • Neural proliferation and differentiation process.
  • Comparison of ES-derived cells with primary cells.
  • High throughput in vitro toxicity testing and screening.

 

Benefits of using NeuroGLO™-Tox HT and NeuroGlo™-Complete
  • Incorporates the most sensitive of any assay readout using proven ATP-based Bioluminomics™ Technology.
  • Instrument-based, quantitative and fully standardized readout procedure that can be validated according to regulatory requirements.
  • Includes cryopreserved hNP1 human neural progenitor cells or hN2 neuronal cells, medium, plates and signal detection system for a complete "turnkey" assay. 
  • High throughput capability.
  • Reliably compare results over time.
  • Multiplexing capability to incorporate other assay readouts for additional information from the same sample.
  • Easy to learn, fast to use.

 Principle, Sensitivity and Parameters of ATP Bioluminescence Assays

Download information on the principle of ATP bioluminescence assays, sensitivity and other parameters

 Species and Cell Sources
  • Human

Stem Cell Sources
  • Embryonic stem (ES) cell-derived hNP1 neural stem/progenitor cells

 Availability of NeuroGLO™

NeuroGLO™ Complete assays are available for:

  • Stem cell research assays with 96-well plates
  • In vitro toxicity screening and testing with 96- or 384-well plates

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

 Assay Kit Contents
  • Cryopreserved vial(s) of hNP1 neural progenitor or hN2 neuronal cells
  • Growth/expansion medium
  • ATP standard
  • ATP controls
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well plates, depending on assay use
  • Non-sterile, 96- or 384-well plates, depending on assay use
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 NeuroGLO™ Quick Guide and Assay Manual

Download the NeuroGLO™ Quick Guide

The NeuroGLO™ Assay Manual can be found in the Resources section here

 Related Assays
Related Assays

STEMFluor™

A Flexible, 96-Well, Fluorescence Assay Kit to Measure Proliferation, Viability and Functionality of ES and iPS Cells, Primary Explanted Cells, Transformed Cell Lines and Cancer Cells. 

 

   Buy STEMFluor™ Assay Kits    

 
 
 
Uses of STEMFluor™-96 Research
  • An alternative to STEMGlo™-96 Research if a luminescence plate reader is not available.
  • A proliferation, viability, functionality and cell number assay for for numerous cells types including primary stem cells, cell lines and primary explanted cells.
  • Developmental biology.
  • Determine self-renewal and expansion ability and potential.
  • Studies on the cell proliferation process.
  • Determination of cells into differentiated cells.
  • Cell-based therapies. 
  • Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other factors on primary (adult) stem cells and stem cell lines.
  • Comparison of ES- or iPS-derived stem cell response with primary stem cells.

 

Benefits of using STEMFluor™-96 Research
  • Uses a fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.
  • Non-subjective, instrument-based and quantitative readout procedure.
    Available for adherent or non-adherent cells.
  • Multiplexes with flow cytometry allowing cells to be detected using other fluorescent labels/markers.
  • Multiplexes with luminescence assays.
  • Multiplexes with gene expression assays etc for more information from a single sample.
  • Flexibility to use virtually any in vitro cell protocol, reagents and growth factors/cytokines.
  • High throughput capability.
  • Fast to learn and easy to use.

 Species and Tissue Sources
  • Multiple

Cell Sources

STEMGlo™-96 Research can be used to study virtually any cell type with proliferation capability or high intracellular ATP concentrations. These include, but are not limited to cells from the following organs and tissues:

  • Bladder
  • Brain
  • Breast
  • Eye
  • Gut
  • Heart
  • Kidney
  • Lung
  • Liver
  • Ovary
  • Prostate
  • Skin
  • Embryonic tissue
  • Fetal tissue.

Other cell types that can be studies using STEMGlo™-96 Research include:

  • Proliferating cell lines
  • Tumor cells
  • Embryonic stem (ES) cells
  • Induced puripotent stem (iPS) cells
  • ES-or iPS-derived cells

 STEMFluor™-96 Research Principle
  • Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
  • After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time: 2 hours.
  • Uses a plate fluorometer or multiparameter plate reader to measure fluorescence with an excitation filter of 380-400nm and an emission filter of 505nm.

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Fluorescence plate reader or multiparameter plate reader.

 Assay Kit Contents
  • GF-AFC Reagent
  • Sterile, 96-well plates, depending on assay use
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 Related Assays

Download the STEMFlour™-96 Research Quick Guide and Assay Manual

The STEMFluor™-96 Research Assay Manual can be found in the Resources section here

 Related Assays

 

STEMLight™

A Flexible, 96-Well, Absorbance Assay Kit to Measure Proliferation, Viability and Functionality of ES and iPS Cells, Primary Explanted Cells, Transformed Cell Lines and Cancer Cells.

  

   Buy STEMLight™ Assay Kits    

 
 
 
Uses of STEMLight™-96 Research
  • An alternative to STEMFluor™-96 Research and STEMGlo™-96 Research using an absorbance plate reader.
  • A proliferation, viability, cell functionality and cell number assays for numerous cell types derived from multiple organs and tissues.
  • Developmental biology.
  • Determine self-renewal and expansion ability and potential.
  • Studies on the cell proliferation process.
  • Determination of cells into differentiated cells.
  • Cell-based therapies. 
  • Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other factors on primary (adult) cells and cell lines.
  • Comparison of ES- or iPS-derived stem cell response with primary stem cells.

 

Benefits of using STEMLight™-96 Research
  • Uses a absorbance plate reader or multiparameter plate reader, available in most laboratories.
  • Non-subjective, instrument-based and quantitative readout procedure.
    Available for adherent or non-adherent cells.
  • Multiplexes with flow cytometric assays.
  • Multiplexes with gene expression assays etc for more information from a single sample.
  • Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
  • High throughput capability.
  • Fast to learn and easy to use.

 Species and Tissue Sources
  • Multiple

Cell Sources

STEMGlo™-96 Research can be used to study virtually any cell type with proliferation capability or high intracellular ATP concentrations. These include, but are not limited to cells from the following organs and tissues:

  • Bladder
  • Brain
  • Breast
  • Eye
  • Gut
  • Heart
  • Kidney
  • Lung
  • Liver
  • Ovary
  • Prostate
  • Skin
  • Embryonic tissue
  • Fetal tissue.

Other cell types that can be studies using STEMGlo™-96 Research include:

  • Proliferating cell lines
  • Tumor cells
  • Embryonic stem (ES) cells
  • Induced puripotent stem (iPS) cells
  • ES-or iPS-derived cells

 STEMLight™-96 Principle
STEMLight™-96 Principle
  • Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction, colorimetric reaction.
  • After culture, just add 20μl of the MTS reagent, mix and read absorbance at 490nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
  • No solubilization step as in a MTT reaction. Replaces MTT assays.
  • Read results between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.

 Use and Major Equipment Required

For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required

Absorbance plate reader or multiparameter plate reader with a 490nm filter.

 Assay Kit Contents
  • MTS Reagent
  • Sterile, 96-well plates
  • Non-sterile, 96-well plate for background determination
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Instruction manual

 STEMLight™-96 Research Quick Guide and Assay Manual

Download the STEMLight™-96 Research Quick Guide

The STEMLight™-96 Assay Manual can be found in the Resources section here

 Related Assays