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STEMpredict.jpg

STEMpredict™ and

STEMpredict™ HT

A specialized HALO® assay to rapidly predict cord blood "quality" and bankability and optimization of mobilized peripheral blood collection.

 

 

Buy STEMpredict™ assay kits or click on the Price tag below

 

 

STEMpredict™ Contract Services

 

 

 See how to setup a STEMpredict™ assays

 

STEMpredict™ Applications
  • Rapidly predict growth functionality and metabolic viability (bankability) of cord blood stem cells in 3 days.
  • Detrmine if a cord blood unit can be permanently banked.
  • Determine if stem cells have been mobilized and optimize mobilized peripheral blood collection.
  • STEMpredict™ is a CFU-substitute assay that is allowed by the FDA.

 

Advantages of Using STEMpredict™ in the Stem Cell Processing Laboratory
 
  • STEMpredict™ is the only assay that can be used for high-throughput, cord blood screening using either 96- or 384-well plate formats.
  • Optimized mobilized peripheral blood collection and use.
  • Predicts stem cell growth, functionality and viability in 3 days.
  • Replaces all other functional assays with non-subjective, quantitative data.
  • Fast, easy to learn and use, and fully standardized allowing sample results to be directly compared with each other.
  • Incorporates ATP Bioluminomics™ technology, the most advanced and sensitive assay readout system available.
  • Incorporates Suspension Expansion Culture™ (SEC™) technology for optimal cell culture, growth and assay sensitivity.
  • Results within 30 min after cell culture.
  • Always reliable and reproducible, with low coefficients of variation (CVs).
  • FDA, AABB or FACT alternative “quality” assay.

 

Please note that STEMpredict™ is not a potency assay. To measure hematopoietic stem cell potency, please see HALO®-96 PQR.

  

STEMpredict™ and STEMpredict™ HT Assays Available

Assay Type Plate Format Catalog Number Number of Plates/Kit

Number of Samples/Kit

Click to see price

STEMpredict 96-well plate

K2-SP-1

1

16

Price
STEMpredict 96-well plate

K2-SP-2

2

32

Price
STEMpredict 96-well plate

K2-SP-4

4

64

Price

STEMpredict HT

384-well plate

K3-SP-4

1

32

Price

STEMpredict HT

384-well plate

K3-SP-4

2

64

Price

STEMpredict HT

384-well plate

K3-SP-4

4

128

Price

To perform STEMpredict™, high throughput, a liquid handler (robot) is required 

 
Tissues used with STEMpredict™
  • Umbilical cord blood
  • Mobilized peripheral blood
  • Bone marrow
  • Purified cells from any of the above

 

 

 

STEMpredict

Click image for larger view


STEMpredict™ for Cord Blood Stem Cells

The graph shows an example of how STEMpredict™ predicts stem cell growth, functionality and viability for umbilical cord blood cells. If cells are metabolically viable (which is not the same as being viable by dye exclusion methods), they will demonstrate cellular and mitochondrial integrity by producing intracellular ATP (iATP). The iATP concentration, in turn, directly correlates with proliferation ability. If a quantitative difference in proliferation ability between stimulated and unstimulated (background) stem cells exists, as shown in the graph, the stem cells from the product are predicted to proliferate and grow. In this way, STEMpredict™ not only detects whether the cells are metabolically viable, but also whether they will exhibit cellular functionality and proliferate. The assay therefore measures three parameters simultaneously.


This same concept and principle is applied to mobilized peripheral blood, bone marrow and purified hematopoietic cell products. It is also the cornerstone of all HemoGenix® cellular therapeutic assay kit products, including HALO®-96 PCAEQ, HALO™-96 SPC-QC, HALO™-96 PMT, MSCGlo™-96 HuQC and MSCGlo™-96 PQR. 

 
Use

For Research Use Only. Not for clinical diagnostic use.

 
Kit Contents
  • STEMpredict™ Master Mix
  • ATP standard
  • ATP high and low controls
  • ATP Enumeration Reagent
  • Sterile, 96- or 384-well plates for cell culture
  • Non-sterile 96-well plates (for assay calibration and standardization)
  • Sterile, adhesive foil covers to maintain sterility in unused wells
  • Instruction manual

 

Related Assays

HALO®-96 SPC-QC

HALO®-96 PQR

HALO®-96 PMT