Proliferating vs Non-Proliferating Cell Systems

 

LumiSTEM

 

Under normal steady-state conditions, there are 5 biological systems of the body that exhibit continuous proliferation throughout life. These are the:

  • Lympho-Hematopoietic system
  • Gastrointestinal system
  • Reproductive system
  • Cells giving rise to the skin
  • Specialized cells of the cornea of the eye.
Some organs can also partially regenerate after damage. The best example is the liver. All other organs and tissues have, up to recently, been considered to be non-proliferating.
 
However, with the comparative recent introduction and availability of human embryonic stem cells, the ability of normally quiescent cells in tissues and organs to exhibit proliferation has gained increased importance. In addition to the ability of tumor cells to exhibit unrestrictve proliferation, different types of cells from virtually every organ and tissue of the body can now be isolated and used as primary target tissue for testing purposes.
 
HemoGenix® developed the LumiSTEM™® Platform specifically to detect these primary proliferating cells from different cell systems. Proliferation of these numerous cell types is dependent upon whether they adhere to a growth surface or not. Thus, LumiSTEM™® is an assay that detects the proliferative status of adherent or non-adherent cells.
 
The types of cells that can be detected and analyzed using the LumiSTEM™® Platform are:
  • Embryonic stem cells and their differentiating progeny
  • Primordial germ cells and their differentiating progeny
  • Cells from the lungs and airways
  • Endothelial cell systems
  • Epithelial cell systems
  • Fibroblast cell systems (not mesenchymal stem cells)
  • Cells from the bladder
  • Cells from the prostate
  • Mammary cells
  • Renal cells
  • Neural cells
  • Transformed cell lines (see Using Cell Lines)

LINKS

List of primary, proliferating cells used by HemoGenix® for its Contract Services