All of the assay platforms that utilize our instrument-based, ATP bioluminescence signal detection system as a readout, can detect and measure either proliferation or inhibition of proliferation (cytotoxicity). Both proliferation and cytotoxicity are proportional to the intracellular ATP concentration. Therefore, growth factors, cytokines, and other perturbations that result in an induction, stimulation or potentiation of the proliferation process can be detected and measured. Similarly, agents that inhibit induction and/or stimulation of the proliferation process can also be detected.
Both proliferation and cytotoxicity can also result in an increase or decrease respectively, in the differentiation program of a cell population. It is important to distinguish whether a proliferation/cytotoxicity or differentiation process is the goal of the research project, since it will depend on the type of assay being used. For example, if a new growth factor is being studied, it may exhibit duel functionality. Under certain conditions or on specific cell populations it might demonstrate proliferative capability, but under other conditions or on other cell populations, it may demonstrate differentiation and/or cell survival or protective capabilities. Using the wrong assay, might lead to a false interpretation and conclusion. However, in many cases, the form of the response curve can provide an indication as to whether a proliferative or differentiation response is being observed. Since cellular differentiation is usually dependent on the proliferative program, a proliferation assay can provide more information than a differentiation assay.
HemoGenix® provides a number of alternatives to differentiation assays for both contract services and in-house testing.
To discuss how we can help you in your research studies, please contact HemoGenix® directly to speak with an expert.