Which HALO® Format is Right for You?

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HemoGenix® has developed three HALO® Platforms:

  • HALO®-96 MeC (Methyl Cellulose)
  • HALO®-96 SEC (Suspension Expansion Culture) and,
  • HALO®-384 HT (High-Throughput)

All HALO® Platforms have the following characteristics in common:

  • Detect proliferation / cytotoxicity - apoptosis.
  • Use a highly sensitive ATP-based bioluminescence readout.
  • Fully calibrated against an external ATP standard performed on a separate plate, thereby allowing standardization of the assay.
  • Non-subjective and DOES NOT require manual colony enumeration, but can be performed if required.
  • Available for human, non-human primate, dog, rat and mouse.
  • Can detect at least 12 different lympho-hematopoietic cell types.
  • Validated against the traditional colony-forming assay.
  • Requires only 3-7 days to obtain results (depending on species and assay platform).
  • Requires only 30 min to process and read a full 96- or 384-well plate.
  • Easy to use with little training required.
  • Conforms to 3R characteristcs: Rapidity, Reliability and Reproducibility.

 

HALO®-96 MeC (Methyl Cellulose)

  • Equivalent to the colony-forming assay with NO colony counting.
  • Clonal growth in methyl cellulose.
  • 100µl volumes in a 96-well plate.
  • 5-7 day turnaround time (depending on species).

Applications:
Direct associations allows HALO®-96 MeC to completely replace the traditional colony-forming assay. HALO® detects the proliferative potential, response and/or status of lympho-hematopoietic cells. Since proliferation occurs prior to differentiation, those cells that proliferate will, by default, also differentiate, providing nothing inhibits this function.

  • Any basic research colony-forming assay can be replaced with HALO®-96 MeC.
  • Calibrated quality control of processed products for stem cell transplantation and cord blood storage.
  • Lead optimization, pre-clinical testing and monitoring during clinical trials of hematopoietic tissues and organs for drug development.

 

HALO®-96 SEC (Suspension Expansion Culture)

  • No methyl cellulose.
  • Easier, more accurate dispensing of reagents leads to lower errors and significantly reduced coefficients of variation.
  • 2-3 times more sensitive than HALO®-96 MeC (depending on cell population assayed).
  • 100µl culture volumes in a 96-well plate.
  • Results in 3-5 days depending on species.
  • Allows for full automation with medium to high throughput capability.

Applications:

Direct associations allows the easier and faster HALO®-96 SEC to replace both methyl cellulose - based assay systems for:
  • All basic research needs to detect and measure lympho-hematopoietic cells from different tissues and organs.
  • Replace the cumbersome and non-standardized colony-forming assay used in stem cell transplantation and cord blood storage processing laboratories for quality control.
  • Screening, lead optimization and pre-clinical studies for drug development.
  • Environmental / xenobiotic testing.
  • Part of the ComparaTOX™ Platform for Predictive In Vitro Cross-Platform Comparative Toxicity testing using LUMENESC™-96 for Mesenchymal Stem Cell toxicity testing and LumiSTEM™-96 for testing toxicity to other primary, proliferating cell systems.

 

HALO®-384 HT (High-Throughput)

  • Suspension Expansion Culture system.
  • 25µl culture volume in a 384-well plate.
  • Higher sensitivity compared to both HALO®-96 MeC and HALO®-96 SEC.

Applications:

  • All hemotoxicity applications requiring full automation with high-throughput capability.
  • Hemotoxicity screening during ADME/Tox for drug discovery.
  • High-throughput environmental / xenobiotic screening and testing.
  • Part of the ComparaTOX™ Platform for Predictive In Vitro Cross-Platform Comparative Toxicity testing using LUMENESC™-384 for Mesenchymal Stem Cell toxicity testing and LumiSTEM™-384 for testing toxicity to other primary, proliferating cell systems.